Radiation induced immunomodulatory effects in head and neck cancers

• Project/Area Number: 26670736
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Gunma University
• Principal Investigator: Chikamatsu Kazuaki 群馬大学, 大学院医学系研究科, 教授 (30301378)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 癌 / 放射線治療 / 免疫学

Outline of Final Research Achievements

Radiotherapy is generally used as one of treatment modalities for head and neck cancer, and it has been known to not only kill cancer cells but also induce anti-tumor immune responses through the expression of immunostimulatory molecules and release various signaling molecules. In this study, immunomodulatory effect by radiotherapy (X-ray and heavy ion radiation) was investigated using head and neck squamous cell carcinoma cell lines. Heavy ion radiation more efficiently killed tumor cells compared to X-ray. Moreover, radiotherapy could enhance calreticulin expression and decrease CD47 expression on tumor cells. These results suggest that radiotherapy might promote successful induction of anti-tumor immune responses via acceleration of phagocytosis by decrease of CD47 molecule.

Research Products

• [Journal Article] Concurrent chemoradiotherapy with conventional fractionated radiotherapy and low-dose daily cisplatin plus weekly docetaxel for T2N0 glottic cancer. (2017)
  • Author(s): Saitoh J, Shirai K, Imaeda M, Musha A, Abe T, Shino M, Takayasu Y, Takahashi K, Chikamatsu K, Nakano T.
  • Journal Title: Radiat Oncol Volume: 12 Issue: 1 Pages: 39-39
  • DOI: 10.1186/s13014-016-0741-4
  • Peer Reviewed
• [Journal Article] Cancer-associated fibroblasts promote an immunosuppressive microenvironment through the induction and accumulation of protumoral macrophages. (2017)
  • Author(s): Takahashi H, Sakakura K, Kudo T, Toyoda M, Kaira K, Oyama T, Chikamatsu K.
  • Journal Title: Oncotarget Volume: 8 Issue: 5 Pages: 8633-8647
  • DOI: 10.18632/oncotarget.14374
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Impact of a multidisciplinary round visit for the management of dysphagia utilizing a Wi-Fi-based wireless flexible endoscopic evaluation of swallowing. (2017)
  • Author(s): Sakakura K, Tazawa M, Otani N, Takagi M, Morita M, Kurosaki M, Chiyoda T, Kanai Y, Endo A, Murata T, Shino M, Yokobori Y, Shirakura K, Wada N, Chikamatsu K.
  • Journal Title: Ann Otol Rhinol Laryngol Volume: 126 Pages: 47-53
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Clinical Outcomes of Definitive and Postoperative Radiotherapy for Stage I-IVB Hypopharyngeal Cancer. (2016)
  • Author(s): Shirai K, Saitoh JI, Musha A, Abe T, Kobayashi D, Takakusagi Y, Takaysu Y, Shino M, Toyoda M, Takahashi K, Chikamatsu K, Ohno T, Nakano T.
  • Journal Title: Anticancer Res Volume: 36 Pages: 6571-6578
  • Peer Reviewed
• [Journal Article] Relationship between tumor-associated macrophage subsets and CD47 expression in squamous cell carcinoma of the head and neck in the tumor microenvironment. (2016)
  • Author(s): Sakakura K, Takahashi H, Kaira K, Toyoda M, Murata T, Ohnishi H, Oyama T, Chikamatsu K.
  • Journal Title: Lab Invest Volume: 96 Issue: 9 Pages: 994-1003
  • DOI: 10.1038/labinvest.2016.70
  • Peer Reviewed
• [Journal Article] Dynamic changes in immune cell profile in head and neck squamous cell carcinoma: Immunomodulatory effects of chemotherapy. (2016)
  • Author(s): Takahashi H, Sakakura K, Mito I, Ida S, Chikamatsu K.
  • Journal Title: Cancer Sci Volume: 107 Issue: 8 Pages: 1065-1071
  • DOI: 10.1111/cas.12976
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Six Cases of Pulmonary <i>Mycobacterium shinjukuense</i> Infection at a Single Hospital (2016)
  • Author(s): Kaira K, Toyoda M, Shimizu A, Imai H, Sakukura K, Suzuki ON, Iijima M, Asao T, Chikamatsu K.
  • Journal Title: Internal Medicine Volume: 55 Issue: 7 Pages: 787-791
  • DOI: 10.2169/internalmedicine.55.5460
  • NAID: 130005141961
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed
• [Journal Article] Expression of ER stress markers (GRP78/BiP and PERK) in adenoid cystic carcinoma. (2016)
  • Author(s): Kaira K, Toyoda M, Shimizu A, Shino M, Sakakura K, Takayasu Y, Takahashi K, Asao T, Chikamatsu K.
  • Journal Title: Acta Otolaryngol Volume: 136 Issue: 1 Pages: 1-7
  • DOI: 10.3109/00016489.2015.1083120
  • Peer Reviewed / Open Access
• [Journal Article] Prediction of acute radiation mucositis using an oral mucosal dose surface model in carbon ion radiotherapy for head and neck tumors. (2015)
  • Author(s): Musha A, Shimada H, Shirai K, Saitoh J, Yokoo S, Chikamatsu K, Ohno T, Nakano T.
  • Journal Title: PLoS ONE Volume: 10 Issue: 10 Pages: e0141734-e0141734
  • DOI: 10.1371/journal.pone.0141734
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Extranodal soft tissue Rosai-Dorfman disease of the head and neck and its diagnositic difficulty. (2015)
  • Author(s): Yajima M, Nakajima K, Hirato J, Chikamatsu K.
  • Journal Title: Auris Nasus Larynx Volume: in press Issue: 3 Pages: 345-349
  • DOI: 10.1016/j.anl.2015.09.008
  • Peer Reviewed
• [Journal Article] Immunosuppressive activity of cancer-associated fibroblasts in head and neck squamous cell carcinoma. (2015)
  • Author(s): Takahashi H, Sakakura K, Kawabata-Iwakawa R, Rokudai S, Toyoda M, Nishiyama M, Chikamatsu K.
  • Journal Title: Cancer Immunol Immunother Volume: 64 Issue: 11 Pages: 1407-1417
  • DOI: 10.1007/s00262-015-1742-0
  • Peer Reviewed
• [Journal Article] Expression of Amino Acid Transporters (LAT1 and ASCT2) in Patients with Stage III/IV Laryngeal Squamous Cell Carcinoma. (2015)
  • Author(s): Nikkuni O, Kaira K, Toyoda M, Shino M, Sakakura K, Takahashi K, Tominaga H, Oriuchi N, Suzuki M, Iijima M, Asao T, Nishiyama M, Nagamori S, Kanai Y, Oyama T, Chikamatsu K.
  • Journal Title: Pathol Oncol Res Volume: 21 Issue: 4 Pages: 1175-1181
  • DOI: 10.1007/s12253-015-9954-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Immunological significance of the accumulation of autophagy components in oral squamous cell carcinoma. (2015)
  • Author(s): Sakakura K, Takahashi H, Kaira K, Toyoda M, Oyama T, Chikamatsu K.
  • Journal Title: Cancer Sci. Volume: 106 Issue: 1 Pages: 1-8
  • DOI: 10.1111/cas.12559
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 18F-FDG uptake on PET correlates with biological potential in early oral squamous cell carcinoma. (2015)
  • Author(s): Yokobori Y, Toyoda M, Sakakura K, Kaira K, Tsushima Y, Chikamatsu K.
  • Journal Title: Acta Otolaryngol Volume: in press Issue: 5 Pages: 494-499
  • DOI: 10.3109/00016489.2014.969385
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] CD98 as a novel prognostic indicator for patients with stage III/IV hypopharyngeal squamous cell carcinoma. (2014)
  • Author(s): Toyoda M, Kaira K, Shino M, Sakakura K, Takahashi K, Takayasu Y, Tominaga H, Oriuchi N, Nikkuni O, Suzuki M, Iijima M, Tsukamoto N, Nagamori S, Kanai Y, Oyama T, Chikamatsu K.
  • Journal Title: Head Neck. Volume: in press Issue: 11 Pages: 1-6
  • DOI: 10.1002/hed.23797
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prognostic significance of amino-acid transporter expression (LAT1, ASCT2, and xCT) in surgically resected tongue cancer. (2014)
  • Author(s): Toyoda M, Kaira K, Ohshima Y, Ishioka NS, Shino M, Sakakura K, Takayasu Y, Takahashi K, Tominaga H, Oriuchi N, Nagamori S, Kanai Y, Oyama T, Chikamatsu K.
  • Journal Title: Br J Cancer. Volume: 110 Issue: 10 Pages: 2506-13
  • DOI: 10.1038/bjc.2014.178
  • Peer Reviewed / Open Access
• [Journal Article] Combined small cell carcinoma of the sinonasal tract associated with syndrome of inappropriated secretion of antidiuretic hormone. (2014)
  • Author(s): Kayakabe M, Takahashi K, Okamiya T, Segawa A, Oyama T, Chikamatsu K.
  • Journal Title: Oncol Lett Volume: 7 Pages: 1253-1256
  • Peer Reviewed / Open Access
• [Presentation] Identification of circulating tumor cells and its clinical relevance in patients with squamous cell carcinoma of the head and neck. (2016)
  • Author(s): Chikamatsu K, Takahashi H, Sakakura K, Shino M, Ida S, Mito I, Toyoda M.
  • Organizer: 9th International Conference on Head and Neck Cancer
  • Place of Presentation: Seattle, USA
  • Year and Date: 2016-07-16
  • Int'l Joint Research
• [Presentation] トランスジェニックカイコによるがん抗原タンパクの作成とがんワクチン療法への応用 (2016)
  • Author(s): 近松一朗, 元川瑶子, 高橋秀行, 坂倉浩一, 武田茂樹
  • Organizer: 第117回日本耳鼻咽喉科学会学術講演会
  • Place of Presentation: 名古屋市
  • Year and Date: 2016-05-19
• [Presentation] Detection and clinical significance of circulating tumor cells in squamous cell carcinoma of the head and neck (2016)
  • Author(s): Chikamatsu K, Takahashi H, Sakakura K, Toyoda M.
  • Organizer: AACR Annual Meeting
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] Significance of differential white blood cell counts on nutrition in oropharyngeal cancer patients with chemoradiation (2015)
  • Author(s): 坂倉浩一、高橋秀行、近松一朗
  • Organizer: 第74回日本癌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2015-10-08
• [Presentation] Possible relation between tumor-associated macrophage (TAM) subsets and CD47 expression on squamous cell carcinoma of the head and neck (SCCHN) in tumor microenvironment (2015)
  • Author(s): Sakakura K, Takahashi H, Kaira K, Toyoda M, Chikamatsu K.
  • Organizer: International Conference of Cancer Immunotherapy and Macrophages
  • Place of Presentation: 東京
  • Year and Date: 2015-07-09
  • Int'l Joint Research
• [Presentation] Carbon ion radiotherapy with concurrent chemotherapy for head and neck mucosal melanoma (2015)
  • Author(s): Chikamatsu K, et al.
  • Organizer: 第4回アジア頭頸部癌学会
  • Place of Presentation: 神戸
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Presentation] The Alteration of Immune Effector and Suppressor Cells in Head and Neck Squamous Cell Carcinoma (2015)
  • Author(s): Takahashi H, Sakakura K, Chikamatsu K.
  • Organizer: 第4回アジア頭頸部癌学会
  • Place of Presentation: 神戸
  • Year and Date: 2015-06-03
  • Int'l Joint Research
• [Presentation] 頭頸部扁平上皮癌患者末梢血中の循環癌細胞の同定と臨床的意義 (2015)
  • Author(s): 近松一朗、高橋秀行、坂倉浩一、岡本彩子
  • Organizer: 第116回日本耳鼻咽喉科学会総会・学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2015-05-20
• [Presentation] Results Of Carbon Ion Radiotherapy For Mucosal Malignant Melanoma Of The Head And Neck (2014)
  • Author(s): Yukihiro Takayasu, Masato Shino, Minoru Toyoda, Katsumasa Takahashi, Kazuaki Chikamatsu, Jun-ichi Saitoh, Katsuyuki Shirai, Tatsuya Ohno, Takashi Nakano
  • Organizer: IFHNOS 5th World Congress And AHNS Annual Meeting
  • Place of Presentation: New York
  • Year and Date: 2014-07-26 – 2014-07-30
• [Presentation] Immunological significance of autophagy components accumulated in oral squamous cell carcinoma (2014)
  • Author(s): Koichi Sakakura, Minoru Toyoda, Tetsunari Oyama, Kazuaki Chikamatsu
  • Organizer: IFHNOS 5th World Congress And AHNS Annual Meeting
  • Place of Presentation: New York
  • Year and Date: 2014-07-26 – 2014-07-30
• [Presentation] 群馬大学における頭頸部領域腺様嚢胞癌に対する重粒子線治療の短期治療成績 (2014)
  • Author(s): 8.高安幸弘，紫野正人、豊田実、近松一朗、斎藤淳一、白井克幸、大野達也、中野隆史
  • Organizer: 第38回日本頭頸部癌学会
  • Place of Presentation: 東京
  • Year and Date: 2014-06-12 – 2014-06-13
• [Presentation] 群馬大学における頭頸部悪性黒色腫に対する重粒子線治療成績 (2014)
  • Author(s): 高安幸弘、紫野正人、豊田実、高橋克昌、近松一朗、齋藤淳一、白井克幸、大野達也、中野隆史
  • Organizer: 第115回日本耳鼻咽喉科学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-05-14 – 2014-05-17
• [Presentation] 口腔扁平上皮癌におけるautophagy関連分子の発現と腫瘍浸潤免疫細胞との関係 (2014)
  • Author(s): 坂倉浩一、高橋秀行、解良恭一、豊田実、小山徹也、近松一朗
  • Organizer: 第115回日本耳鼻咽喉科学会総会
  • Place of Presentation: 福岡市
  • Year and Date: 2014-05-14 – 2014-05-17