| Project/Area Number |
26670740
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KANNO MASAFUMI 福井大学, 医学部附属病院, 助教 (90444215)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | ES細胞 / iPS細胞 / neurosphere / 神経幹細胞 / 嗅覚 / FGF2 / 嗅覚障害 / 嗅神経 / 多能性幹細胞 |
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| Outline of Final Research Achievements |
The recovery from smell disturbance is difficult at present. The study on the disorder has hardly advanced for the last several decades and the disease is referred to as an important issue of modern medicine.The purpose of this research was to make a neurosphere from an embryonic stem cell and induced pluripotent stem cells efficiently and to cure the sense of smell of a mouse with smell disturbance by transplanting it.
We succeeded in the formation of a primary neurosphere through the floating culture method which was the serum-free medium including FGF2. Furthermore, we dispersed and subcultured it and succeeded in the inducement to the second and third neurospheres. We transplanted the third neurosphere in a model with smell disturbance, but unfortunately the improvement of the sense of smell reaction was not recognized. The reason seemed to be that the number of transplant cells was too small.
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