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Glycoblotting analysis of N-glycans related to diabetic retinopathy

Research Project

Project/Area Number 26670749
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Ophthalmology
Research InstitutionHokkaido University

Principal Investigator

ISHIDA Susumu  北海道大学, 医学(系)研究科(研究院), 教授 (10245558)

Co-Investigator(Kenkyū-buntansha) NISHIMURA Shin-Ichiro  北海道大学, 先端生命科学研究科, 教授 (00183898)
NODA Kousuke  北海道大学, 大学院医学研究科, 准教授 (90296666)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords糖尿病網膜症 / 糖鎖 / グライコミクス
Outline of Final Research Achievements

Since the prevalence of diabetic retinopathy, severe ocular complication of diabetes, is closely related to the duration of diabetes, with the growing diabetes epidemic the world would experience an explosion in the number of patients with DR. However, despite extensive basic research and clinical studies, biochemical mechanisms responsible for the pathogenesis of DR remains elusive. Lines of evidence have indicated that structural alterations of N-glycans are related to the pathogenesis of diseases, and thus the profile changes of N-glycans in ocular diseases such as DR are of great interest.
In the present study, we demonstrated that i) the vitreous N-glycan concentration was significantly higher in patients with DR than those without diabetes, ii) sialylated N-glycans were increased in the vitreous fluid of patients with DR, and iii) high glucose stimulation augmented the sialyltransferases ST3GAL1 and ST3GAL4, but not ST6GAL1.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (40 results)

All 2016 2015 2014

All Journal Article (22 results) (of which Peer Reviewed: 10 results,  Open Access: 8 results,  Acknowledgement Compliant: 1 results) Presentation (17 results) (of which Int'l Joint Research: 4 results,  Invited: 4 results) Book (1 results)

  • [Journal Article] Vitreous levels of vascular endothelial growth factor-B in proliferative diabetic retinopathy2016

    • Author(s)
      Kinoshita S, Noda K, Saito W, Kanda A, Ishida S
    • Journal Title

      Acta Ophthalmol

      Volume: - Issue: 6

    • DOI

      10.1111/aos.12969

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increase of Sialylated N-glycans in Eyes with Neovascular Glaucoma Secondary to Proliferative Diabetic Retinopathy2016

    • Author(s)
      Inafuku S., Noda K., Amano M., Nishimura S-I., Ishida S.
    • Journal Title

      Curr. Eye Res.

      Volume: 印刷中 Pages: 1-4

    • DOI

      10.3109/02713683.2015.1068816

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alteration of N-glycan profiles in Diabetic Retinopathy2015

    • Author(s)
      Inafuku S., Noda K., Amano M., Murata M., Saito W., Ohashi T., Kanda A., Nishimura S-I., Ishida S.
    • Journal Title

      Invest. Ophthalmol. Vis. Sci.

      Volume: 56 Issue: 9 Pages: 5316-5322

    • DOI

      10.1167/iovs.15-16747

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aflibercept traps galectin-1, an angiogenic factor associated with diabetic retinopathy.2015

    • Author(s)
      Kanda A, Noda K, Saito W, Ishida S
    • Journal Title

      Sci Rep

      Volume: 5 Issue: 1 Pages: 17946-17946

    • DOI

      10.1038/srep17946

    • NAID

      120005690890

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 網膜細胞生物学と分子標的療法 – ポストVEGF創薬の時代. 巻頭言(総説)2015

    • Author(s)
      石田 晋
    • Journal Title

      日眼会誌

      Volume: 119 Pages: 759-760

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 網膜疾患におけるVEGFの生物活性とVEGF阻害薬の特色(総説)2015

    • Author(s)
      石田 晋
    • Journal Title

      日本の眼科

      Volume: 86 Pages: 1088-1094

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 糖尿病網膜症における炎症と血管新生の分子病態(総説)2015

    • Author(s)
      石田 晋
    • Journal Title

      日本糖尿病学会誌

      Volume: 19 Pages: 56-60

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 抗VEGF薬の基礎研究と開発の歴史(総説)2015

    • Author(s)
      野田航介
    • Journal Title

      あたらしい眼科

      Volume: 32 Pages: 1069-1073

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 知っておきたい糖尿病網膜症 (DR) の2大病態. 合併症 網膜症(総説)2015

    • Author(s)
      野田航介
    • Journal Title

      プラクティス

      Volume: 32 Pages: 13-15

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 近年における糖尿病網膜症 (DR) 診療機器の進歩. 合併症 網膜症(総説)2015

    • Author(s)
      野田航介
    • Journal Title

      プラクティス

      Volume: 32 Pages: 132-135

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 糖尿病網膜症 (DR) に対する分子標的治療 - 抗VEGF療法. 合併症 網膜症(総説)2015

    • Author(s)
      野田航介
    • Journal Title

      プラクティス

      Volume: 32 Pages: 260-262

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 糖尿病網膜症と基礎研究2015

    • Author(s)
      野田航介
    • Journal Title

      月刊糖尿病

      Volume: 7 Pages: 14-18

    • Related Report
      2015 Annual Research Report
  • [Journal Article] VAP-1の糖尿病網膜症における基礎研究(総説)2015

    • Author(s)
      眞島行彦、野田航介
    • Journal Title

      別冊Bio Clinica「慢性炎症と疾患」

      Volume: 4 Pages: 131-137

    • Related Report
      2015 Annual Research Report
  • [Journal Article] ATP6AP2/(pro)renin receptor contributes to glucose metabolism via stabilizing the pyruvate dehydrogenase E1 b-subunit.2015

    • Author(s)
      Kanda A, Noda K, Ishida S.
    • Journal Title

      J Biol Chem.

      Volume: 290 Issue: 15 Pages: 9690-9700

    • DOI

      10.1074/jbc.m114.626713

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 糖尿病網膜症における炎症と血管新生の分子病態2015

    • Author(s)
      石田 晋
    • Journal Title

      日本糖尿病学会誌

      Volume: 19 Pages: 56-60

    • Related Report
      2014 Research-status Report
  • [Journal Article] A comparison of N-glycan profiles in human plasma and vitreous fluid.2014

    • Author(s)
      Inafuku S, Noda K, Amano M, Ohashi, Yoshizawa C, Saito W, Kanda A, Nishimura S, Ishida S.
    • Journal Title

      Graefes Arch Clin Exp Ophthalmol.

      Volume: 252 Issue: 8 Pages: 1235-1243

    • DOI

      10.1007/s00417-014-2671-x

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Aqueous humor levels of placental growth factor in diabetic retinopathy2014

    • Author(s)
      Ando R, Noda K, Namba S, Saito W, Kanda A, Ishida S
    • Journal Title

      Acta Ophthalmol

      Volume: 92 Issue: 3 Pages: 245-246

    • DOI

      10.1111/aos.12251

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genistein attenuates choroidal neovascularization2014

    • Author(s)
      Kinoshita S, Noda K, Tagawa Y, Inafuku S, Dong Y, Fukuhara J, Dong Z, Ando R, Kanda A, Ishida S
    • Journal Title

      J Nutr Biochem

      Volume: 25 Issue: 11 Pages: 1177-1182

    • DOI

      10.1016/j.jnutbio.2014.06.004

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Viteous renin-angiotensin system in proliferative diabetic retinopathy2014

    • Author(s)
      Ishida S
    • Journal Title

      Folia Japonica de Ophthalmologica Clinica

      Volume: 7 Pages: 844-846

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] 日本における視覚障害の原因と現状. 糖尿病黄斑浮腫患者を対象としたペガプタニブナトリウムの国内第III相臨床試験2014

    • Author(s)
      石橋達朗、湯澤美都子、吉村長久、大路正人、石田 晋、五十川直樹、江坂悦子
    • Journal Title

      日本眼科学会雑誌

      Volume: 118 Pages: 773-782

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] VEGF阻害薬によって薬理作用の強さは異なるのか?2014

    • Author(s)
      石田 晋
    • Journal Title

      眼科

      Volume: 56 Pages: 883-890

    • Related Report
      2014 Research-status Report
  • [Journal Article] 糖尿病網膜症の分子病態と薬物治療2014

    • Author(s)
      石田 晋
    • Journal Title

      日本眼科学会雑誌

      Volume: 118 Pages: 607-618

    • Related Report
      2014 Research-status Report
    • Open Access
  • [Presentation] Choroidal circulation in uveitis. Symposia & Courses: Inflammation studies2016

    • Author(s)
      Ishida S
    • Organizer
      Asia-Pacific Academy of Ophthalmology Congress (APAO) 2016
    • Place of Presentation
      Taipei(Taiwan)
    • Year and Date
      2016-03-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Afilibercept traps Galectin-1, an angiogenic factor associated with diabetic retinopathy2016

    • Author(s)
      Ishida S
    • Organizer
      39th Annual Macula Society Meeting
    • Place of Presentation
      Florida (USA)
    • Year and Date
      2016-02-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒト硝子体中におけるN 型糖鎖の解析. シンポジウム「臨床検体から網膜硝子体疾患を理解する」2015

    • Author(s)
      野田航介
    • Organizer
      第69回日本臨床眼科学会
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-10-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] アフリベルセプト硝子体内投与の糖尿病網膜症に対する効果2015

    • Author(s)
      石田 晋
    • Organizer
      第69回日本臨床眼科学会
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2015-10-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Alteration of N-glycan profiles in diabetic retinopathy2015

    • Author(s)
      Noda K
    • Organizer
      48th Annual retina society scientific meeting
    • Place of Presentation
      Paris (France)
    • Year and Date
      2015-10-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 糖尿病網膜症の硝子体および血漿におけるN型糖鎖プロファイル2015

    • Author(s)
      稲福沙織、野田航介、天野麻穂、大橋 哲、齋藤 航、村田美幸、神田敦宏、西村紳一郎、石田 晋
    • Organizer
      第36回日本炎症・再生医学会
    • Place of Presentation
      虎ノ門ヒルズフォーラム(東京都・特別区)
    • Year and Date
      2015-07-21
    • Related Report
      2015 Annual Research Report
  • [Presentation] Alteration of N-glycan profiles in diabetic retinopathy2015

    • Author(s)
      Inafuku S, Noda K, Amano M, Murata M, Saito W, Ohashi T, Kanda A, Nishimura S, Ishida S
    • Organizer
      ARVO (Association for Research in Vision and Ophthalmology) Annual Meeting
    • Place of Presentation
      Denver(USA)
    • Year and Date
      2015-05-03
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 糖負荷刺激によるシアル酸転移酵素の誘導2015

    • Author(s)
      稲福沙織、野田航介、天野麻穂、大橋 哲、齋藤 航、村田美幸、神田敦宏、西村紳一郎、石田 晋
    • Organizer
      第119回日本眼科学会総会
    • Place of Presentation
      ロイトン札幌(北海道・札幌市)
    • Year and Date
      2015-04-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] 糖尿病網膜症の硝子体および血漿におけるN型糖鎖プロファイル2015

    • Author(s)
      稲福沙織、野田航介、天野麻穂、大橋哲、齋藤 航、村田美幸、神田敦宏、西村紳一郎、石田 晋
    • Organizer
      第20回日本糖尿病眼学会総会
    • Place of Presentation
      ソラシティカンファレンスセンター(東京都千代田区)
    • Year and Date
      2015-03-06 – 2015-03-08
    • Related Report
      2014 Research-status Report
  • [Presentation] Role of VEGF as a pro-inflammatory cytokine in diabetic macular edema. Symposium: Inflammatory mechanisms in diabetic retinopathy and other retiral degeneration2015

    • Author(s)
      Ishida S
    • Organizer
      Asia-ARVO 2015
    • Place of Presentation
      パシフィコ横浜(横浜市西区)
    • Year and Date
      2015-02-18
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Receptor-associated prorenin system in the pathogenesis of diabetic retinopathy2015

    • Author(s)
      Ishida S
    • Organizer
      Asia-ARVO 2015
    • Place of Presentation
      パシフィコ横浜(横浜市西区)
    • Year and Date
      2015-02-17
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Alteration of N-glycan profiles in diabetic retinopathy2015

    • Author(s)
      Inafuku S, Noda K, Amano M, Murata M, Saito W, Ohashi T, Kanda A, Nishimura S, Ishida S
    • Organizer
      Asia-ARVO 2015
    • Place of Presentation
      パシフィコ横浜(横浜市西区)
    • Year and Date
      2015-02-16 – 2015-02-19
    • Related Report
      2014 Research-status Report
  • [Presentation] ATP6AP2/ (pro)renin receptor contributes to glucose metabolism via stabilizing the E1 β subunit of pyruvate dehydrogenase complex2015

    • Author(s)
      Kanda A, Noda K, Ishida S
    • Organizer
      Asia-ARVO 2015
    • Place of Presentation
      パシフィコ横浜(横浜市西区)
    • Year and Date
      2015-02-16 – 2015-02-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 糖尿病網膜症の硝子体および血漿におけるN型糖鎖の網羅的解析2014

    • Author(s)
      稲福沙織、野田航介、天野麻穂、大橋哲、吉澤史子、齋藤 航、村田美幸、神田敦宏、西村紳一郎、石田 晋
    • Organizer
      第68回日本日本臨床眼科学会
    • Place of Presentation
      神戸ポートピアホテル(神戸市中央区)
    • Year and Date
      2014-11-13 – 2014-11-16
    • Related Report
      2014 Research-status Report
  • [Presentation] Profile of N-glycans in human vitreous2014

    • Author(s)
      Inafuku S, Noda K, Amano M, Ohashi T, Yoshida S, Saito W, Kanda A, Nishimura S, Ishida S
    • Organizer
      第79回日本インターフェロン・サイトカイン学会学術集会
    • Place of Presentation
      北海道大学医学部フラテ会館(札幌市北区)
    • Year and Date
      2014-06-19 – 2014-06-20
    • Related Report
      2014 Research-status Report
  • [Presentation] Profile of N-glycans in human vitreous2014

    • Author(s)
      Takashina S, Noda K, Amano M, Ohashi T, Dong Y, Kinoshita S, Saito W, Kanda A, Nishimura S, Ishida S
    • Organizer
      ARVO (Association for Research in Vision and Ophthalmology) Annual Meeting
    • Place of Presentation
      Florida(USA)
    • Year and Date
      2014-05-04 – 2014-05-08
    • Related Report
      2014 Research-status Report
  • [Presentation] Profile analysis of glycans in human vitreous2014

    • Author(s)
      Takashina S, Noda K, Amano M, Nishimura S, Ishida S
    • Organizer
      World Ophthalmology Congress 2014
    • Place of Presentation
      東京国際フォーラム(東京都千代田区)
    • Year and Date
      2014-04-02 – 2014-04-04
    • Related Report
      2014 Research-status Report
  • [Book] Neuroprotection and Regeneration for Retinal Diseases.2014

    • Author(s)
      Kanda A, Ishida S
    • Total Pages
      356
    • Publisher
      Springer
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2017-05-10  

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