| Project/Area Number |
26670750
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Iwate University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SUGANO Eriko 岩手大学, 工学部, 准教授 (70375210)
TABATA Kitako 岩手大学, 工学部, 技術補佐員 (80714576)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生理学 / 悩・神経 / 再生医学 / 遺伝子治療 / 視覚誘発電位 / チャネルロドプシン / 網膜色素変性症 |
|---|
| Outline of Final Research Achievements |
The aim of this study is to utilize the halorhodopsin (NpHR) gene derived from Halobacterium salinarum for producing OFF responses in the photoreceptor degenerated retina. We modified the amino acid sequence of NpHR (mNpHR) to express it into a mammalian cell. The mNpHR expression was clearly observed in the retina after the AAV2 mediated mNpHR gene transfer. However we could not get the expected responses such as the light induced OFF responses. We investigated the localization of mNpHR in the cell. The mNpHR expression was mainly observed in the endoplasmic reticurum but not in the cell membrane.
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