• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Treatment of diabetic macular edema by VEGF-SiRNA using ligand-inducible system

Research Project

Project/Area Number 26670752
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Ophthalmology
Research InstitutionShinshu University

Principal Investigator

MURATA Toshinori  信州大学, 学術研究院医学系, 教授 (50253406)

Co-Investigator(Kenkyū-buntansha) IESATO Yasuhiro  信州大学, 医学部, 助教 (00708357)
Research Collaborator IMAI Akira  信州大学, 大学院医学系研究科, 大学院生
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords糖尿病黄斑浮腫 / VEGF / 遺伝子改変マウス / RNA干渉 / 発現誘導システム
Outline of Final Research Achievements

The aim of the present study was to develop a new method to suppress overexpression of vascular endothelial growth factor (VEGF) using SiRNA to treat experimentally induced diabetic retinopathy-like changes. To evaluate the effects of the treatment, we established a method to obtain high resolution images of fluorescein angiography and optical coherence tomography. To induce gene transduction using retroviral vector, we also developed a method to deliver laser burn in mouse retinas to induce cell proliferation that is necessary for gene transduction using this viral vector.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 4 results) Presentation (2 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Physiological significance of recombination-activating gene 1 in neuronal death, especially optic neuropathy.2015

    • Author(s)
      Hirano T, Murata T, Hayashi T.
    • Journal Title

      FEBS J

      Volume: 282 Issue: 1 Pages: 129-141

    • DOI

      10.1111/febs.13109

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] En face swept-source optical coherence tomography detecting thinning of inner retinal layers as an indicator of capillary nonperfusion2015

    • Author(s)
      Imai A, Toriyama Y, Iesato Y, Hirano T, Murata T
    • Journal Title

      Eur J Ophthalmol

      Volume: 25 Issue: 2 Pages: 153-158

    • DOI

      10.5301/ejo.5000514

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Laser wavelength on delivering appropriate laser burns through the opaque lens using a pattern scan laser.2014

    • Author(s)
      Hirano T, Iesato Y, Imai A, Toriyama Y, Kikushima W, Murata T.
    • Journal Title

      Ophthalmic Res

      Volume: 51 Issue: 4 Pages: 204-209

    • DOI

      10.1159/000357918

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Detection of fovea-threatening diabetic macular edema by optical coherence tomography to maintain good vision by prophylactic treatment2014

    • Author(s)
      Hirano T, Iesato Y, Toriyama Y, Imai A, Murata T.
    • Journal Title

      Ophthalmic Res

      Volume: 52 Issue: 2 Pages: 65-73

    • DOI

      10.1159/000362372

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Correlation between diabetic retinopathy severity and elevated skin autofluorescence as a marker of advanced glycation end-product accumulation in type 2 diabetic patients2014

    • Author(s)
      Hirano T, Iesato Y, Toriyama Y, Imai A, Chiba D , Murata T.
    • Journal Title

      Journal of Diabetes and Its Complications

      Volume: 28 Pages: 729-34

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] A multi-center clinical study evaluating the effectiveness of intravitreal ranibizumab in combination with photocoagulation for diabetic macular edema2015

    • Author(s)
      Takao Hirano Toshinori Murata
    • Organizer
      The 8th Joint Meeting of Japan-China-Korea Ophthalmologists
    • Place of Presentation
      Hilton FUKUOKA SEA HAWK, Fukuoka, Japan
    • Year and Date
      2015-10-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparison of Pnaretinal Navigated and Conventional Pattern Scan Laser Photocoagulation for Diabetic Retinopathy2015

    • Author(s)
      Yuichi Toriyama, Toshinori Murata
    • Organizer
      The 8th Joint Meeting of Japan-China-Korea Ophthalmologists
    • Place of Presentation
      Hilton FUKUOKA SEA HAWK, Fukuoka, Japan
    • Year and Date
      2015-10-09
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi