Treatment of diabetic macular edema by VEGF-SiRNA using ligand-inducible system
| Project/Area Number |
26670752
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IESATO Yasuhiro 信州大学, 医学部, 助教 (00708357)
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| Research Collaborator |
IMAI Akira 信州大学, 大学院医学系研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 糖尿病黄斑浮腫 / VEGF / 遺伝子改変マウス / RNA干渉 / 発現誘導システム |
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| Outline of Final Research Achievements |
The aim of the present study was to develop a new method to suppress overexpression of vascular endothelial growth factor (VEGF) using SiRNA to treat experimentally induced diabetic retinopathy-like changes. To evaluate the effects of the treatment, we established a method to obtain high resolution images of fluorescein angiography and optical coherence tomography. To induce gene transduction using retroviral vector, we also developed a method to deliver laser burn in mouse retinas to induce cell proliferation that is necessary for gene transduction using this viral vector.
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Report
(3 results)
Research Products
(7 results)
