Detectino of AGEs in the basement membrane in the eye, and establishment of the AGE detection clinical equipments.

• Project/Area Number: 26670755
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Kyushu University (2015-2016); Yamaguchi University (2014)
• Principal Investigator: SONODA Koh-Hei 九州大学, 医学研究院, 教授 (10294943)
• Co-Investigator(Kenkyū-buntansha): 柳井 亮二 山口大学, 医学部附属病院, 講師 (10346554) ; 森重 直行 山口大学, 医学(系)研究科(研究院), 講師 (40346565)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 眼組織 / AGE / 老年期障害 / サファイアレーザー / 内皮基底膜由来の強散乱光 / 最終投下産物 / ヘモグロビン / 基底膜散乱 / 生体検査 / 非侵襲的検出 / 最終糖化産物 / 非侵略的検出

Outline of Final Research Achievements

We tried to detect AGEs in the basement membrane in the eye, and establish the AGE detection clinical equipments. AGE can cause organ dysfunction in the various conditions, abnormalities of the basement membrane are thought to contribute to the complications of aging. Eye ball must be the easiest organ to detect AGE in the whole body. We established the best conditioned wavelength in mode-fixed titanium sapphire laser light source. It was also confirmed that by using an objective lens with a working distance of 30 mm or more, the retina and the lens are easy to evaluate.

Research Products

• [Journal Article] Coordinated Regulation of Palladin and α-Smooth Muscle Actin by Transforming Growth Factor-β in Human Corneal Fibroblasts. (2016)
  • Author(s): Morishige N, Murata S, Nakamura Y, Azumi H, Shin-Gyou-Uchi R, Oki KT, Morita Y, Sonoda KH.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 57 Issue: 7 Pages: 3360-8
  • DOI: 10.1167/iovs.15-18763
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Up-regulation of semaphorin 4A expression in human retinal pigment epithelial cells by PACAP released from cocultured neural cells (2015)
  • Author(s): Ko JA, Hirata J, Yamane K, Sonoda KH, Kiuchi Y.
  • Journal Title: Cell Biochem Funct Volume: 33 Issue: 1 Pages: 29-36
  • DOI: 10.1002/cbf.3082
  • Peer Reviewed
• [Journal Article] Development and validation of new diagnostic criteria for acute retinal necrosis. (2015)
  • Author(s): Takase H, Okada AA, Goto H, Mizuki N, Namba K, Ohguro N, Sonoda KH, Tomita M, Keino H, Kezuka T, Kubono R, Mizuuchi K, Shibuya E, Takahashi H, Yanai R, Mochizuki M
  • Journal Title: Jpn J Ophthalmol Volume: 59(1) Issue: 1 Pages: 14-20
  • DOI: 10.1007/s10384-014-0362-0
  • NAID: 210000165197
  • Peer Reviewed / Open Access
• [Journal Article] Quantitative Analysis of Collagen Lamellae in the Normal and Keratoconic Human Cornea by Second Harmonic Generation Imaging Microscopy (2014)
  • Author(s): Morishige N，Shin-gyou-uchi R，Azumi H，Ohta H，Morita Y，Yamada N，Kimura K，Takahara A，Sonoda KH
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 55 Issue: 12 Pages: 8377-8385
  • DOI: 10.1167/iovs.14-15348
  • Peer Reviewed
• [Journal Article] A stem cell medium containing neural stimulating factor induces a pancreatic cancer stem-like cell-enriched population. (2014)
  • Author(s): Watanabe Y, Yoshimura K, Yoshikawa K, Tsunedomi R, Shindo Y, Matsukuma S, Maeda N, Kanekiyo S, Suzuki N, Kuramasu A, Sonoda K, Tamada K, Kobayashi S, Saya H, Hazama S and Oka M
  • Journal Title: Int J Oncol Volume: 45 Issue: 5 Pages: 1857-1866
  • DOI: 10.3892/ijo.2014.2603
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of the long-term efficacy and safety of infliximab treatment for uveitis in Behçet's disease: a multicenter study. (2014)
  • Author(s): Takeuchi M, Kezuka T, Sugita S, Keino H, Namba K, Kaburaki T, Maruyama K, Nakai K, Hijioka K, Shibuya E, Komae K, Hori J, Ohguro N, Sonoda KH, Mizuki N, Okada AA, Ishibashi T, Goto H, Mochizuki M
  • Journal Title: Ophthalmology Volume: 121(10) Issue: 10 Pages: 1877-1884
  • DOI: 10.1016/j.ophtha.2014.04.042
  • Peer Reviewed / Open Access
• [Journal Article] EBI3 is pivotal for the initiation of experimental autoimmune uveitis. (2014)
  • Author(s): Takeda A, Hasegawa E, Fukuhara T, Hirakawa S, Yamada H, Yang Y, Yoshimura T, Hisatomi T, Oshima Y, Yoshida H, Sonoda KH, Ishibashi T
  • Journal Title: Exp Eye Res Volume: 125 Pages: 107-113
  • DOI: 10.1016/j.exer.2014.06.004
  • Peer Reviewed
• [Journal Article] Cytochrome P450-generated metabolites derived from ω-3 fatty acids attenuate neovascularization (2014)
  • Author(s): Yanai R，Mulki L，Hasegawa E，Takeuchi K，Sweigard H，Suzuki J，Gaissert P，Vavvas DG，Sonoda KH，Rothe M，Schunck WH，Miller JW ，Connor KM
  • Journal Title: Proc Natl Acad Sci USA Volume: 111 Issue: 26 Pages: 9603-9608
  • DOI: 10.1073/pnas.1401191111
  • Peer Reviewed
• [Journal Article] Congenital hypoplastic trigeminal nerve revealed by manifestion of corneal disorders likely caused by neural factor deficiency (2014)
  • Author(s): Morishige N，Morita Y，Yamada N，Nishida T，Sonoda KH
  • Journal Title: Case Rep Ophthalmol Volume: 5 Issue: 2 Pages: 181-185
  • DOI: 10.1159/000364899
  • Peer Reviewed
• [Journal Article] Peters' anomaly imaged with an infrared anterior segment camera (2014)
  • Author(s): Morishige N，Morita Y，Yamada N，Sonoda KH
  • Journal Title: Clin Exp Ophthalmol Volume: 42 Issue: 4 Pages: 391-392
  • DOI: 10.1111/ceo.12186
  • Peer Reviewed
• [Presentation] The role of IL-27 in the formation of ocular inflammatory disease (2015)
  • Author(s): Sonoda KH
  • Organizer: 2015 Asia-ARVO
  • Place of Presentation: 神奈川県横浜市（パシフィコ横浜）
  • Year and Date: 2015-02-18
  • Invited
• [Presentation] 高齢者の非感染性ぶどう膜炎は眼局所治療で治す！ (2014)
  • Author(s): 園田 康平
  • Organizer: 第48回日本眼炎症学会
  • Place of Presentation: 東京都千代田区（東京国際フォーラム）
  • Year and Date: 2014-07-05
  • Invited
• [Presentation] 自然免疫系が修飾する後眼部疾患 (2014)
  • Author(s): 園田 康平
  • Organizer: 第48回日本眼炎症学会
  • Place of Presentation: 東京都千代田区（東京国際フォーラム）
  • Year and Date: 2014-07-05
  • Invited
• [Presentation] Primary Care for Patients with Uveitis (Learning from Clinical Uveitic Cases) (2014)
  • Author(s): 園田 康平
  • Organizer: WOC2014
  • Place of Presentation: 東京都千代田区（東京国際フォーラム）
  • Year and Date: 2014-04-04
  • Invited