Development of novel anitiboday targeting periostin for the treatment of proliferative vitreoretinal diseases

• Project/Area Number: 26670757
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Kyushu University
• Principal Investigator: Yoshida Shigeo 九州大学, 医学(系)研究科(研究院), 准教授 (50363370)
• Co-Investigator(Kenkyū-buntansha): ISHIBASHI TATSURO 九州大学, 大学病院, 病院長 (30150428) ; 星野 友 九州大学, 工学(系)研究科(研究院), 准教授 (40554689)
• Co-Investigator(Renkei-kenkyūsha): HOSHINO YU 九州大学, 工学研究院, 准教授 (40554689)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: プラスチック抗体 / 網膜血管新生 / 脈絡膜血管新生 / ペリオスチン / ナノ粒子 / 血管新生

Outline of Final Research Achievements

We synthesized polymer nanoparticle libraries and performed screening to extract those polymer particles to inhibit periostin-dependent cell proliferation using in vitro cell proliferation assays. At the 2nd screening, we could extract Cation1-4, Cation1-5, Cation1-6 which inhibited cell proliferation in a dose-dependent manner. Among these polymer nanoparticles, Cation1-4 exhibited the significant inhibition of retinal and choroidal proliferation in vivo.

Research Products

• [Journal Article] M2 Macrophages Enhance Pathological Neovascularization in the Mouse Model of Oxygen-Induced Retinopathy (2015)
  • Author(s): 1Zhou Y, Yoshida S, Nakao S, Yoshimura T, Kobayashi Y, Nakama T, Kubo Y, Miyawaki K, Yamaguchi M, Ishikawa K, Oshima Y, Akashi K,Ishibashi T
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 56 Issue: 8 Pages: 4767-77
  • DOI: 10.1167/iovs.14-16012
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Inhibition of choroidal fibrovascular membrane formation by new class of RNA interference therapeutic agent targeting periostin. (2015)
  • Author(s): Nakama T, Yoshida S, Ishikawa K, Kobayashi Y, Zhou Y, Nakao S, Sassa Y, Oshima Y, Takao K, Shimahara A, Yoshikawa K, Hamasaki T, Ohgi T, Hayashi H, Matsuda A, Kudo A, Nozaki M, Ogura Y, Kuroda M, Ishibashi T.
  • Journal Title: Gene Therapy. Volume: 22 Issue: 2 Pages: 127-137
  • DOI: 10.1038/gt.2014.112
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 眼内血管新生におけるマトリセルラー蛋白の役割 (2015)
  • Author(s): 吉田 茂生
  • Organizer: Retina Research Forum
  • Place of Presentation: 東京
  • Year and Date: 2015-10-04
  • Int'l Joint Research / Invited
• [Presentation] Novel molecular-targeted therapies for intraocular proliferation, (2015)
  • Author(s): Shigeo Yoshida
  • Organizer: 7th Chinese Congress of Research in Vision and Ophthalmology
  • Place of Presentation: Shenyang
  • Year and Date: 2015-08-14
  • Int'l Joint Research / Invited
• [Presentation] 網膜血管新生とマクロファージ (2015)
  • Author(s): 吉田 茂生
  • Organizer: 第119回日本眼科学会
  • Place of Presentation: 札幌
  • Year and Date: 2015-04-18
  • Invited
• [Presentation] 増殖組織特徴遺伝子ペリオスチン標的一本鎖核酸による網膜血管新生抑制 (2015)
  • Author(s): 中間崇仁、吉田茂生、他
  • Organizer: 第119回日本眼科学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2015-04-16