| Project/Area Number |
26670767
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Hokkaido University |
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Principal Investigator |
FURUKAWA Hiroshi 北海道大学, 医学(系)研究科(研究院), 准教授 (00399924)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Yuhei 北海道大学, 大学院医学研究科, 教授 (70271674)
OYAMA Akihiko (FUNAYAMA Emi) 北海道大学, 北海道大学病院, 講師 (10533630)
HAYASHI Toshihiko 北海道大学, 大学院歯学研究科, 准教授 (00432146)
MURAO Naoki 北海道大学, 北海道大学病院, 助教 (90706558)
SHICHINOHE Ryuji 北海道大学, 北海道大学病院, 医員 (30640346)
小山 明彦 北海道大学, 大学病院, 講師 (70374486)
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| Research Collaborator |
ONO Kentaro 北海道大学, 北海道大学病院, 医員
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 末梢神経 / 軸索再生 / neurotization |
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| Outline of Final Research Achievements |
This work aimed to validate whether axonal regeneration would be possible through the venous network in the muscle. At first, we planned to use the latissimus dorsi muscle dominated by thoracodorsal nerve and vein, but we changed experimental animal model because of surgical difficulty. Eventually, we used saphenous nerve and femoral vein at inside of the thigh. The saphenous nerve was cut and inserted into the femoral vein, and two months later, we removed specimen compounded of nerve and vein. The specimen was stained with NF-H and CD34 by immunostaining procedure and observed to ascertain that axonal regeneration occurred in the vein. Unfortunately, we couldn’t identify the regeneration of axons. This study indicated that the distal condition which was the target of axonal elongation was of the same importance as scaffold of axonal regeneration.
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