Establishment of immortalized cell lines of keloid fibroblasts

• Project/Area Number: 26670776
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Jichi Medical University
• Principal Investigator: SUNAGA Ataru 自治医科大学, 医学部, 助教 (00406117)
• Project Period (FY): 2014-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: ケロイド

Outline of Final Research Achievements

We introduced hTERT gene to human keloid-derived fibroblasts and established 48 immortalized cell lines.
After 20 passages, we identified 5 true keloid fibroblast cell clones which maintained the highest collagen type 1 expression.