| Project/Area Number |
26670782
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Yamagata University |
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Principal Investigator |
Goto Kaoru 山形大学, 医学部, 教授 (30234975)
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| Co-Investigator(Kenkyū-buntansha) |
秋元 亮 山形大学, 医学部, 助教 (40594677)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | エネルギー代謝 / 脂質代謝酵素 / 低酸素 / エネルギーセンサー / ATP / 熱ショックタンパク質 / エネルギーストレス / ミトコンドリア / 呼吸 / ATP産生 / 低酸素ストレス / HIF / 細胞内情報伝達機構 / 培養細胞 / ジアシルグリセロールキナーゼ / 細胞内情報伝達系 / 塩化コバルト |
|---|
| Outline of Final Research Achievements |
Upon tissue damage, hypothermia induces Hypoxia inducible factor (HIF), which suppresses energy expenditure and exerts a beneficial effect to damaged cells. In this study we examined the regulatory mechanism of the diacylglycerol kinase (DGK) family on HIF expression and energy metabolism. We found that after 24 h of 1% hypoxic conditions DGKzeta deficiency reduces HIF1alpha expression by 50%, activates energy sensor AMPK, and increases intracellular ATP levels. These results suggest that DGKzeta plays an important role in energy metabolism under hypoxic conditions.
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