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Osteoclastogenesis from embryonic macrophages: Novel cell fate analysis and regulation of bone metabolism

Research Project

Project/Area Number 26670803
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Morphological basic dentistry
Research InstitutionKyushu University

Principal Investigator

Kukita Toshio  九州大学, 歯学研究科(研究院), 教授 (70150464)

Co-Investigator(Kenkyū-buntansha) KUKITA AKIKO  佐賀大学, 医学部, 准教授 (30153266)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords破骨細胞 / 胎生期マクロファージ / Fate Mapping解析 / アンチセンスRNA / 新生仔破骨細胞 / Fate Mapping 解析 / 骨代謝制御 / Fate Mapping 解析 / 血島
Outline of Final Research Achievements

Purpose of this research is to verify a hypothesis that osteoclasts are derived from macrophages observed in the embryonic stages. As we could not obtain mice required for performing Fate Mapping analysis of the early embryonic macrophages, we have changed research strategy. We have characterized the properties of osteoclasts present in newborn animals, which are supposed to be formed from cells in macrophages-lineage present in embryos. We have focused on investigating an involvement of WT1, zinc-finger transcription factor having the ability to induce macrophage differentiation, in the generation of osteoclasts present in newborn rats. We have detected a marked expression of antisense RNA for WT1(WT1asRNA)in active osteoclasts present in newborn rats. Over-expression of WT1asRNA in osteoclast precursors significantly augmented osteoclastogenesis. These data show that osteoclastogenesis is controlled by WT1asRNA at least in osteoclastogenesis from fetal osteoclast precursors.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (14 results)

All 2016 2015 2014

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 6 results) Presentation (5 results) Book (1 results)

  • [Journal Article] Interferon-gamma improves impaired dentinogenic and immunosuppressive functions of irreversible pulpitis-derived human dental pulp stem cells.2016

    • Author(s)
      Sonoda S, Yamaza H, Ma L, Tanaka Y, Tomoda E, Aijima R, Nonaka K, Kukita T, Shi S, Nishimura F, Yamaza T.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 19286-19286

    • DOI

      10.1038/srep19286

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Membrane Nanotube Formation in Osteoclastogenesis.2015

    • Author(s)
      Kukita T., Takahahsi A., Zhang J-Q., Kukita A.
    • Journal Title

      Kurt Pfannkuche (ed), Cell Fusion: Overviews and Methods, Methods in Molecular Biology

      Volume: 1313 Pages: 193-202

    • DOI

      10.1007/978-1-4939-2703-6_14

    • ISBN
      9781493927029, 9781493927036
    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inhibition of RANKL-dependent cellular fusion in pre-osteoclasts by amiloride and a NHE10-specific monoclonal antibody.2015

    • Author(s)
      Mine Y, Shuto T, Nikawa H, Kawai T, Ohara M, Kawahara K, Ohta K, Kukita T, Terada Y, Makihira S.
    • Journal Title

      Cell Biol Int.

      Volume: 39 Issue: 6 Pages: 696-709

    • DOI

      10.1002/cbin.10447

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 炎症性骨破壊の免疫学的制御:病的骨破壊のみを制御する「安全かつ強力な次世代型骨代謝制御法」の開発をめざして2015

    • Author(s)
      久木田敏夫、久木田明子
    • Journal Title

      臨床免疫・アレルギー科(科学評論社)

      Volume: 64(2) Pages: 113-120

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Involvement of deoxyadenosine and adenosine deaminase in the Methotrexate-induced suppression of inflammatory bone destruction.2015

    • Author(s)
      Qu P-F., Kukita A., Li Y-J., Moriyama K., Lei L., Kukita T.
    • Journal Title

      In: Adenosine Receptors: Pharmacology, Functions and Therapeutic aspects. Ed. Warrick K.,

      Volume: ISBN: 978-1-63463-454-0 Pages: 143-164

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of CXCL14 in osteolytic bone metastasis from lung cancer.2014

    • Author(s)
      Takiguchi S, Korenaga N, Inoue K, Sugi E, Kataoka Y, Matsusue K, Futagami K, Li YJ, Kukita T, Teramoto N, Iguchi H.
    • Journal Title

      Int J Oncol.

      Volume: 44(4) Issue: 4 Pages: 1316-1324

    • DOI

      10.3892/ijo.2014.2293

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulation of osteoclastogenesis through Tim-3: possible involvement of the Tim-3/galectin-9 system in the modulation of inflammatory bone destruction.2014

    • Author(s)
      K. Moriyama, A. Kukita, Y-J. Li, N. Uehara, J-Q Zhang, I. Takahashi, T. Kukita
    • Journal Title

      Lab. Invest

      Volume: 94(11) Issue: 11 Pages: 1200-1211

    • DOI

      10.1038/labinvest.2014.107

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mesenchymal stem cells markedly suppress inflammatory bone destruction in rats with adjuvant-induced arthritis2014

    • Author(s)
      Takano, T. Li, Y. J. Kukita, A. Yamaza, T. Ayukawa, Y. Moriyama, K. Uehara, N. Nomiyama, H. Koyano, K. and Kukita, T.
    • Journal Title

      Laboratory Investigation

      Volume: 94 Issue: 3 Pages: 286-296

    • DOI

      10.1038/labinvest.2013.152

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ラミニン322によるRANK発現調節とマクロファージ-破骨細胞分化転換の制御2015

    • Author(s)
      上原範久、久木田明子、久本由香里、保田尚孝、久木田敏夫
    • Organizer
      第33回日本骨代謝学会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Galectin-9による破骨細胞分化制御メカニズムの解明:NFATc1の発現を抑制する転写因子MafB及びIRF-8の関与2015

    • Author(s)
      久本由香里、上原範久、久木田明子、久木田敏夫
    • Organizer
      第33回日本骨代謝学会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] Ndd4ユビキチンリガーゼ結合タンパク質Pmepa1の破骨細胞の分化と機能における新たな役割2015

    • Author(s)
      徐祥赫、舟久保立、白木誠、久木田敏夫、久木田明子
    • Organizer
      第33回日本骨代謝学会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 免疫調節膜表面分子Tim-3の破骨細胞での発現と炎症性骨破壊制御2014

    • Author(s)
      森山加奈子、久木田明子、上原範久、久本由香里、高橋一郎、久木田敏夫
    • Organizer
      第56回 歯科基礎医学会
    • Place of Presentation
      福岡
    • Year and Date
      2014-09-24 – 2014-09-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 免疫調節膜表面分子Tim-3を介する炎症性骨破壊制御2014

    • Author(s)
      森山加奈子、久木田明子、上原範久、久本由香里、高橋一郎、久木田敏夫
    • Organizer
      第32回 日本骨代謝学会
    • Place of Presentation
      大阪
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Research-status Report
  • [Book] ADENOSINE RECEPTORS Pharmacology, Functions and Therapeutic Aspects: Involvement of Deoxyadenosine and Adenosine Deaminase in the Methotrexate-induced Suppression of Inflammatory Bone Destruction2015

    • Author(s)
      Peng-fei Qu, Akiko Kukita, Yin-ji Li, Kanako Moriyama, Lin Lei, Toshio Kukita
    • Total Pages
      23
    • Publisher
      Nova Science Publishers, Inc. New York
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2017-05-10  

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