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The presence and its significance of non-canonical action of decoy receptors

Research Project

Project/Area Number 26670808
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Functional basic dentistry
Research InstitutionOkayama University

Principal Investigator

TAKIGAWA MASAHARU  岡山大学, 医歯(薬)学総合研究科, 教授 (20112063)

Co-Investigator(Kenkyū-buntansha) KUBOTA SATOSHI  岡山大学, 大学院医歯薬学総合研究科, 教授 (90221936)
AOYAMA ERIKO  岡山大学, 大学院医歯薬学総合研究科, 助教 (10432650)
NISHIDA TAKASHI  岡山大学, 大学院医歯薬学総合研究科, 准教授 (30322233)
HATTORI TAKAKO  岡山大学, 大学院医歯薬学総合研究科, 助教 (00228488)
Co-Investigator(Renkei-kenkyūsha) TAKAESU KAZUMI  岡山大学, 大学院医歯薬学総合研究科, 助教 (10457228)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsdecoy receptor / osteoprotegerin (OPG) / PDGFRL / CCN family / CCN2 / signaling / osteoclasts / chondrocytes / デコイ受容体 / 細胞分化 / 細胞増殖 / CCNファミリー / 軟骨 / 血小板 / CD302 / CCN / OPG / RANK / 破骨細胞 / 軟骨細胞
Outline of Final Research Achievements

In this study, we proposed a new concept showing the presence and its significance of non-canonical action of decoy receptor (-like) molecules by demonstrating 2 examples. 1) Osteoprotegerin (OPG) bound to CCN family protein 2 (CCN2), which binds to RANK and positively regulates RALK signaling, thereby inhibiting osteoclastogenesis via RANK signaling. 2) Platelet-derived growth factor receptor-like (PDGFRL) did not bind to PDGF which is the ligand for PDGF. Instead, PDGFRL did bind to CCN2 which plays important roles in chondrogenesis and endochondral ossification and another member of CCN family CCN3. These findings suggest that PDGFRL plays an important role in the cartilage biology, possibly by regulating the molecular behavior of CCN2. 3) We also found that c-type lectin receptor CD302 bound to CCN2, suggesting possible discovery of another example which supports our new concept.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (32 results)

All 2016 2015 2014 Other

All Journal Article (10 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 9 results,  Open Access: 1 results) Presentation (19 results) (of which Invited: 1 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Involvement of multiple CCN family members in platelets that support regeneration of joint tissues2016

    • Author(s)
      Hara C., Kubota S., Nishida T., Hiasa M., Hattori T., Aoyama E., Moriyama Y., Kamioka H., Takigawa M.
    • Journal Title

      Mod. Rheumatol.

      Volume: 26 Pages: 1-32

    • NAID

      120006358661

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The CCN Proteins:An overview2016

    • Author(s)
      Takigawa M.
    • Journal Title

      Methods Mol. Biol.

      Volume: 未定

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Analysis of transcytosis of CCN2 by chondrocytes2016

    • Author(s)
      Kawata K., Kubota S. and Takigawa M.
    • Journal Title

      Methods Mol. Biol.

      Volume: 未定

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Evaluation of molecular interaction between CCN2 protein and its binding partners by surface plasmon resonance (SPR)2016

    • Author(s)
      Aoyama E. and Takigawa M.
    • Journal Title

      Methods Mol. Biol.

      Volume: 未定

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Protocols for screening for binding partners of CCN proteins: Yeast two- hybrid system2016

    • Author(s)
      Hoshijima M., Hattori T., and Takigawa M.
    • Journal Title

      Methods Mol. Biol.

      Volume: 未定

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Cell biological assays for measuring chondrogenic activities of CCN proteins2016

    • Author(s)
      Nishida T., Kubota S. and Takigawa M.
    • Journal Title

      Methods Mol. Biol.

      Volume: 未定

    • Related Report
      2015 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Physical interaction of CCN2 with diverse growth factors involved in chondrocyte differentiation during endochondral ossification2015

    • Author(s)
      Khattab HM, Aoyama E, Kubota S, Takigawa M
    • Journal Title

      J. Cell Commun. Signal.

      Volume: 未定 Issue: 3 Pages: 247-54

    • DOI

      10.1007/s12079-015-0290-x

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Cellular and molecular actions of CCN2/CTGF and their role under physiological and pathological conditions2015

    • Author(s)
      Kubota S. and Takigawa M.
    • Journal Title

      Clinical Science(invited review)

      Volume: 128(3) Issue: 3 Pages: 181-196

    • DOI

      10.1042/cs20140264

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] CCN family protein 2 (CCN2) promotes the early differentiation, but inhibits the terminal differentiation of skeletal myoblasts2015

    • Author(s)
      Nishida T, Kubota S, Aoyama E, Janune D, Lyons KM, Takigawa M.
    • Journal Title

      J Biochem

      Volume: 157(2) Issue: 2 Pages: 91-100

    • DOI

      10.1093/jb/mvu056

    • NAID

      40020353020

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] CCN2 enhances RANKL-induced osteoclast differentiation via direct binding to RANK and OPG2015

    • Author(s)
      Aoyama E, Kubota S, Khattab HM, Nishida T, Takigawa M.
    • Journal Title

      Bone

      Volume: 73 Pages: 242-248

    • DOI

      10.1016/j.bone.2014.12.058

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 軟骨細胞分化における癌抑制遺伝子PDGFR-like (PDGFRL)の役割2016

    • Author(s)
      河田かずみ、久保田聡、江口傑徳、青山絵理子、森谷徳文、岡 森彦、川木晴美、滝川正春
    • Organizer
      第29回日本軟骨代謝学会
    • Place of Presentation
      広島
    • Year and Date
      2016-02-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] 成熟破骨細胞のアクチンリング形成におけるCD302の機能とCCN2による制御2015

    • Author(s)
      青山絵理子、星島光博、服部高子、久保田聡、滝川正春
    • Organizer
      第38回分子生物学会年会・第88回生化学会大会合同大会BMB2015
    • Place of Presentation
      神戸
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 破骨細胞分化における新規アクチン骨格制御因子としてのDCL-1/CD302の役割とCCN2との関連2015

    • Author(s)
      青山絵理子、星島光博、服部高子、久保田聡、滝川正春
    • Organizer
      第57回歯科基礎医学会
    • Place of Presentation
      新潟
    • Year and Date
      2015-09-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] interaction of CCN2 with varied growth factors and cytokines involved in chondrocyte differentiation during endochondral ossification2015

    • Author(s)
      Khattab H.M., Aoyama E., Kubota S., Takigawa M.
    • Organizer
      第7回日本CCNファミリー研究会
    • Place of Presentation
      岡山
    • Year and Date
      2015-08-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] CCN2によるTRAIL誘導性アポトーシス促進作用2015

    • Author(s)
      青山絵理子、久保田聡、滝川正春
    • Organizer
      第7回日本CCNファミリー研究会
    • Place of Presentation
      岡山
    • Year and Date
      2015-08-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] CCNファミリー研究の歴史と最前線2015

    • Author(s)
      滝川正春
    • Organizer
      第33回日本骨代謝学会 Meet the Experts
    • Place of Presentation
      東京
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 新たな破骨細胞制御因子DCL-1/CD302の作用機構の解明とCCN2との関連2015

    • Author(s)
      青山絵理子、服部高子、星島光博、久保田聡、滝川正春
    • Organizer
      第33回日本骨代謝学会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 巨核球および血小板に存在するCCNファミリータンパク質の存在様態とその由来2015

    • Author(s)
      原 規子、久保田聡 、青山絵理子、上岡 寛、滝川正春
    • Organizer
      第33回日本骨代謝学会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-23
    • Related Report
      2015 Annual Research Report
  • [Presentation] 破骨細胞の成熟を制御する新規膜タンパク質CD302/DCL-1の機能とCCN2との結合2015

    • Author(s)
      青山絵理子、服部高子、星島光博、久保田聡、滝川正春
    • Organizer
      第6回骨バイオサイエンス研究会
    • Place of Presentation
      岡山
    • Year and Date
      2015-07-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] 成熟破骨細胞の形成におけるCD302/DCL-1の新機能とCCN2との関連2015

    • Author(s)
      青山絵理子、星島光博、服部高子、久保田聡、滝川正春
    • Organizer
      第1回日本骨免疫学会
    • Place of Presentation
      沖縄
    • Year and Date
      2015-06-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 血小板に存在するCCNファミリーメンバーとその由来2014

    • Author(s)
      原 規子、久保田聡 、青山絵理子、滝川正春
    • Organizer
      第35回岡山歯学会学術集会
    • Place of Presentation
      岡山
    • Year and Date
      2014-10-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 破骨細胞分化における新規CCN2結合タンパク質DCL-1の発現と機能2014

    • Author(s)
      青山絵理子、星島光博、服部高子、久保田聡、滝川正春
    • Organizer
      第87回日本生化学会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 血小板に存在するCCNファミリータンパク質とその役割2014

    • Author(s)
      原 規子、久保田聡 、青山絵理子、滝川正春
    • Organizer
      第87回日本生化学会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] 新たなCCN2結合因子DCL-1の破骨細胞分化における役割2014

    • Author(s)
      青山絵理子、星島光博、服部高子、久保田聡、滝川正春
    • Organizer
      第56回歯科基礎医学会
    • Place of Presentation
      福岡
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 血小板に存在するCCNファミリーメンバーの存在様態2014

    • Author(s)
      原 規子、久保田聡 、青山絵理子、滝川正春
    • Organizer
      第6回日本CCNファミリー研究会
    • Place of Presentation
      岡山
    • Year and Date
      2014-08-30
    • Related Report
      2014 Research-status Report
  • [Presentation] RANKデコイレセプターOPGとCCN2の相互作用2014

    • Author(s)
      青山絵理子、西田 崇、久保田聡、滝川正春
    • Organizer
      第6回日本CCNファミリー研究会
    • Place of Presentation
      岡山
    • Year and Date
      2014-08-30
    • Related Report
      2014 Research-status Report
  • [Presentation] CCN2結合因子DCL-1の破骨細胞分化制御因子としての役割2014

    • Author(s)
      青山絵理子、服部高子、滝川正春
    • Organizer
      第32回日本骨代謝学会
    • Place of Presentation
      大阪
    • Year and Date
      2014-07-24 – 2014-07-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 血小板に含まれるマトリセルラーCCNファミリータンパク質2014

    • Author(s)
      原規子、久保田聡、青山絵里子、滝川正春
    • Organizer
      第46回日本結合組織学会学術大会・第61回マトリックス研究会大会合同学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-06-05 – 2014-06-07
    • Related Report
      2014 Research-status Report
  • [Presentation] CCN2 induces osteoclastogenesis by regulating RANK/RANKL/OPG system2014

    • Author(s)
      Aoyama E, Kubota S, Nishida T, and Takigawa M
    • Organizer
      ECTS Congress 2014
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2014-05-17 – 2014-05-20
    • Related Report
      2014 Research-status Report
  • [Book] CCN Proteins: Methods and Protocols2016

    • Author(s)
      Takigawa M.
    • Publisher
      Springer
    • Related Report
      2015 Annual Research Report
  • [Remarks] 岡山大学歯学部先端領域研究センター(ARCOCS)

    • URL

      http://www.dent.okayama-u.ac.jp/arcocs/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 岡山大学 歯学部先端領域研究センター(ARCOCS)

    • URL

      http://www.dent.okayama-u.ac.jp/arcocs/

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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