Development of an in vitro model of oral lichen planus by utilizing a characteristic of immune-privilege
| Project/Area Number |
26670817
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Niigata University |
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Principal Investigator |
IZUMI KENJI 新潟大学, 医歯学系, 教授 (80242436)
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| Co-Investigator(Kenkyū-buntansha) |
ITO AKIKO 新潟大学, 医歯学総合病院, 講師 (80361898)
AKIBA YOSUKE 新潟大学, 医歯学総合病院, 講師 (70547512)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 口腔扁平苔癬 / 口腔粘膜上皮細胞 / CD8陽性T細胞 / インビトロモデル / 共培養 / 免疫特権 / CD８陽性T細胞 |
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| Outline of Final Research Achievements |
We detected oral mucosa keratinocytes cultured in a tissue culture dish do not express FasL by immunocytochemistry, FACS and RT-PCR analyses. The pharmacological induction of FasL expression was not successful, either. This resulted in the difficulty in developing an in vitro model of oral lichen planus by utilizing a characteristic of immune-privilege. However, we found that approximately 10% of human T cells expressed FasL. This suggestied it may be possible to analyze the T cell-oral keratinocyte pathophysiological interation in vitro.
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Report
(3 results)
Research Products
(1 results)
