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The study on the effects of Semaphorin3A on dentin regeneration using dental pulp stem cells

Research Project

Project/Area Number 26670826
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Conservative dentistry
Research InstitutionKyushu University

Principal Investigator

Wada Naohisa  九州大学, 大学病院, 教授 (60380466)

Co-Investigator(Kenkyū-buntansha) 赤峰 昭文  九州大学, 歯学研究院, 教授 (00117053)
前田 英史  九州大学, 歯学研究院, 教授 (10284514)
門野内 聡  九州大学, 歯学研究院, 助教 (30609558)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords歯髄細胞 / 象牙質再生 / 露髄 / beta-catenin
Outline of Final Research Achievements

In cases of pulp exposure, existing pulp-capping materials have a limited ability to reconstruct dentin-pulp complexes. Therefore, the development of more effective therapeutic agents has been anticipated for direct pulp capping. We investigated the effects of semaphorin 3A (Sema3A) on various functions of human DPSCs in vitro and reparative dentin formation in a rat dental pulp exposure model. Sema3A induced cell migration, chemotaxis, proliferation, and odontoblastic differentiation of DPSC clones. In addition, Sema3A treatment of DPSC clones increased β-catenin nuclear accumulation. Furthermore, in the rat dental pulp exposure model, Sema3A promoted reparative dentin formation with dentin tubules and a well-aligned odontoblast-like cell layer almost completely covering pulp tissue.
These findings suggest that Sema3A could play an important role in dentin regeneration via canonical Wnt/β-catenin signaling. Sema3A might be an alternative agent for direct pulp capping.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (15 results)

All 2017 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (9 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Book (4 results)

  • [Journal Article] Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells.2017

    • Author(s)
      Serita S, Tomokiyo A, Hasegawa D, Hamano S, Sugii H, Yoshida S, Mizumachi H, Mitarai H, Monnouchi S, Wada N, Maeda H.
    • Journal Title

      Arch Oral Biol.

      Volume: 78 Pages: 135-143

    • DOI

      10.1016/j.archoralbio.2017.02.018

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells.2016

    • Author(s)
      Yoshida S, Wada N, Hasegawa D, Miyaji H, Mitarai H, Tomokiyo A, Hamano S, Maeda H.
    • Journal Title

      Journal of Dental Research

      Volume: 95 Issue: 11 Pages: 1282-1290

    • DOI

      10.1177/0022034516653085

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Transgelin mediates TGF-beta1-induced human periodontal ligament cell proliferation.2017

    • Author(s)
      Mitarai H, Wada N, Hasegawa D, Yoshida S, Mai S, Tomokiyo A, Hamano S, Maeda H
    • Organizer
      95th General Session & Exhibition of the IADR
    • Place of Presentation
      Moscone West, San Francisco, CA, USA
    • Year and Date
      2017-03-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Semaphorin 3A induces odontoblastic phenotype in dental pulp stem cells.2016

    • Author(s)
      Yoshida S, Wada N, Hasegawa D, Tomokiyo A, Hamano S, Mitarai H, Hideki S, Maeda H.
    • Organizer
      94th General Session & Exhibition of the IADR
    • Place of Presentation
      CoEx Convention & Exhibition Center, Seoul, Korea
    • Year and Date
      2016-06-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Semaphorin 3A induces odontoblastic phenotype in dental pulp stem cells.2016

    • Author(s)
      Shinichiro Yoshida, Naohisa Wada, Daigaku Hasegawa, Atsushi Tomokiyo, Sayuri Hamano, Hiromi Mitarai, Hideki Sugii, Hidefumi Maeda
    • Organizer
      94th General Session & Exhibition of the IADR
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2016-06-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 歯髄細胞におけるβig-h3の発現および機能について2016

    • Author(s)
      芹田俊、友清淳、長谷川大学、濱野さゆり、杉井英樹、吉田晋一郎、水町博之、御手洗裕美、和田尚久、前田英史
    • Organizer
      第144回日本歯科保存学会春季学術大会
    • Place of Presentation
      栃木県総合分化センター(宇都宮市)
    • Year and Date
      2016-06-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] Semaphorin 3A induces odontoblastic phenotype in dental pulp stem cells.2016

    • Author(s)
      Yoshida S, Wada N, Hasegawa D, Tomokiyo A, Hamano S, Mitarai H, Serita S, Mizumachi H, Maeda H.
    • Organizer
      Kyudai Oral Bioscience 2016 -Frontiers in Dental Research and Education in East Asia-
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2016-02-27
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 炎症性サイトカインで刺激したヒト歯根膜細胞由来のGDNFはPC12の神経細胞分化を 促進する。2015

    • Author(s)
      吉田晋一郎、山本直秀、和田尚久、友清淳、長谷川大学、濱野さゆり、祐田明香、 御手洗裕美、杉井英樹、前田英史
    • Organizer
      第143回日本歯科保存学会秋季学術大会
    • Place of Presentation
      東京都
    • Year and Date
      2015-11-12
    • Related Report
      2015 Research-status Report
  • [Presentation] Wnt5aはRor2-JNKシグナルを介してヒト歯根膜幹細胞株の骨芽細胞様分化を抑制する2014

    • Author(s)
      1.長谷川大学、和田尚久、前田英史、吉田晋一郎、御手洗裕美、門野内聡、濱野さゆり、祐田明香、赤峰昭文
    • Organizer
      第141回日本歯科保存学会秋季学術大会
    • Place of Presentation
      山形テルサ(山形市)
    • Year and Date
      2014-10-30 – 2014-10-31
    • Related Report
      2014 Research-status Report
  • [Presentation] 歯根膜幹細胞の多様な特性およびその有用性2014

    • Author(s)
      和田尚久
    • Organizer
      第56回歯科基礎医学会学術大会 サテライトシンポジウム「間葉系幹細胞の直接的/間接的な組織再生への関与を考える」
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Year and Date
      2014-09-25
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Semaphorin3Aがヒト歯髄幹細胞による硬組織形成に及ぼす影響2014

    • Author(s)
      4.吉田晋一郎、和田尚久、前田英史、門野内聡、長谷川大学、御手洗裕美、濱野さゆり、祐田明香、杉井英樹、赤峰昭文
    • Organizer
      第140回日本歯科保存学会春季学術大会
    • Place of Presentation
      滋賀県立芸術劇場(大津市)
    • Year and Date
      2014-06-19 – 2014-06-20
    • Related Report
      2014 Research-status Report
  • [Book] Future Perspectives in Dental Stem Cell Engineering and the Ethical Considerations. In: Dental Stem Cells2016

    • Author(s)
      Wada N, Tomokiyo A, Maeda H
    • Publisher
      Springer International Publishing Switzerland
    • Related Report
      2016 Annual Research Report
  • [Book] Contribution of stem cells to dental tissue regeneration: isolation, function, and application. In: Frontiers in stem cell and regeneration medicine research vol.22016

    • Author(s)
      Tomokiyo A, Wada N, Maeda H
    • Publisher
      Bentham Science Publishers
    • Related Report
      2016 Annual Research Report
  • [Book] Frontiers in stem cell and regeneration medicine research vol.22015

    • Author(s)
      Tomokiyo A, Wada N, Maeda H.
    • Total Pages
      294
    • Publisher
      Bentham Science Publishers
    • Related Report
      2015 Research-status Report
  • [Book] Dental Stem Cells2015

    • Author(s)
      Wada N, Tomokiyo A, Maeda H
    • Total Pages
      328
    • Publisher
      Springer
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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