Construction of mesenchymal stem cells-calcium phosphate hybrid spheroids using an oxygen-permeable cell culture device
| Project/Area Number |
26670846
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
鈴木 治 東北大学, 歯学研究科, 教授 (60374948)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生体材料学 / 細胞培養デバイス / 間葉系幹細胞 / リン酸カルシウム |
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| Outline of Final Research Achievements |
Three-dimensional (3D) cellular constructs have been paid attention in the field of tissue engineering. Increase in size of cellular aggregates causes a depletion of oxygen in the core of aggregates. We have developed an oxygen-permeable cell culture device to prevent hypoxia in the core of the cellular aggregates. The aim of this study was construction of 3D cellular structure consisted of mesenchymal stem cells (MSCs) and calcium phosphate materials based on the oxygen-permeable device we developed. We found that the device was able to improve osteoblastic differentiation of MSCs and generate hybrid spheroids consisted of cells and calcium phosphates. These results suggest that the device is useful for engineering 3D cellular constructs for tissue engineering.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Effect of resorption rate and osteoconductivity of biodegradable calcium phosphate materials on the acquisition of natural bone strength in the repaired bone2016
Author(s)
S Chiba, T Anada, K Suzuki, K Saito, Y Shiwaku, N Miyatake, Baba, H Imaizumi, M Hosaka, E Itoi, O Suzuki
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Journal Title
J Biomed Mater Res A
Volume: 104
Issue: 11
Pages: 2833-2842
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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