| Project/Area Number |
26670854
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OHGA Noritaka 北海道大学, 大学院歯学研究科, 助教 (40548202)
SATO Jun 北海道大学, 大学院歯学研究科, 講師 (60319069)
ASAKA Takuya 北海道大学, 大学院歯学研究科, 助教 (80637265)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | フローサイトメトリー / 口腔内科 / 唾液 / 細胞 |
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| Outline of Final Research Achievements |
Objective: Confirmation of a flow cytometry-based method for the determination of major leucocyte subsets such as leukocyte, monocytes, T cells and B cells in stimulated whole human saliva (SWS). Study Design: Salivary leucocyte subsets were determined by three-colour flow cytometry (Aria-Ⅱ) in five healthy volunteers . Comparison of leucocyte subsets between saliva and whole blood was also performed. It was observed that mononuclear cells were the predominant cells in the saliva. Proportions of CD45 positive cells in psWHS. Most of CD45 positive cells belongs to lymphocyte fraction in FSC-SSC plot. When calculated as a percentage of all CD45+ events in saliva, the most frequent were Th cells (CD4+), T cells (CD3+).
Flow cytometry can be used as non-invasive and reproducible method for the analysis of leucocyte subsets in human saliva. Further investigation of pathological conditions such as oral candidiasis and lichen planus and other conditions .
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