| Project/Area Number |
26670865
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
Okamoto Tetsuji 広島大学, 医歯薬保健学研究院(歯), 教授 (00169153)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 無血清培養 / 顎骨・歯胚誘導 / iPS細胞 / センダイウイルスベクター / 無フィーダー培養 / 鎖骨頭蓋異形成症 / Noonan症候群 / 基底細胞母斑症候群 / 疾患特異的iPS細胞 / 口腔顎顔面遺伝性疾患 / 顎顔面口腔組織再生 / エンブリオボディー形成 / Noonan syndrome / ヒトiPS細胞 / 軟骨・骨形成 / 未分化性 / 多分化能 / ３胚葉分化 / 無血清培地（hESF9） |
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| Outline of Final Research Achievements |
We have successfully established and long-term cultured iPS cells from the pulp cells and peripheral blood mononuclear cells derived from Cleido cranial dysplasia, Noonan, VonRecklinghousendisease, basal nevoid cell carcinoma syndrome in serum-, feeder-,and integration-free culture. These cells exhibited several pluripotent gene expression and differentiation abilities in vitro and in vivo.The teratoma tissues derived from CCD-iPS cells and Noonan-iPA cells exhibited abnormal cartilage structures similar to the patient's cartilage. These results strongly suggest that disease-specific iPS cells might be very useful to elucidate mechanism these diseases.
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