Regulation of epithelial polarization to regenerate tooth

• Project/Area Number: 26670880
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: FUKUMOTO SATOSHI 東北大学, 歯学研究科, 教授 (30264253)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: エナメル質 / ３次元培養 / 石灰化 / 歯の再生 / 上皮ー間葉相互作用

Outline of Final Research Achievements

Enamel formation is known as a process that involves cellular proliferation, differentiation through sequential epithelial-mesenchymal interactions, secretion of tissue-specific matrix proteins (e.g., amelogenin), active conveyance of inorganic ions including calcium, and the onset of precipitation and orientation of enamel apatite cystals with enamel matrices. To form artificial enamel, in vitro differentiation system of ameloblast is useful to regenerate tooth and hard tissue. Here, we established new 3D culture system to induce ameloblast differentiation and calcification. A novel 3D culture for dental epithelial cell line SF2 induced enamel specific extracellular matrix, a meloblastin and amelogenin. Further, calcification was observed in this culture system indicating that this new culture method might be useful to form artificial enamel in vitro.

Research Products

• [Journal Article] Nephronectin plays critical roles in Sox2 expression and proliferation in dental epithelial stem cells via EGF-like repeat domains. (2017)
  • Author(s): Arai C, Yoshizaki K, Miyazaki K, Saito K, Yamada A, Han X, Funada K, Fukumoto E, Haruyama N, Iwamoto T, Takahashi I, Fukumoto S.
  • Journal Title: Sci Rep. Volume: 27 Issue: 1 Pages: 45181-45181
  • DOI: 10.1038/srep45181
  • NAID: 120006534986
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model (2017)
  • Author(s): Ono M, Oshima M, Ogawa M, Sonoyama W, Hara ES, Oida Y, Shinkawa S, Nakajima R , Mine A ,Hayano S, Fukumoto S , Kasugai8 S , Yamaguchi A, Tsuji T, Kuboki T
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 44522
  • DOI: 10.1038/srep44522
  • NAID: 120006535050
  • URL: https://tokushima-u.repo.nii.ac.jp/records/2005583
  • Peer Reviewed / Open Access
• [Journal Article] <b>Effect of 1,25-dihydroxyvitamin D</b><b>₃</b><b> on spontaneous calcium responses in rat dental epithelial SF2 cells revealed by long-term </b><b>imaging </b> (2016)
  • Author(s): Murata K, Takahashi A, Morita T, Nezu A, Fukumoto S, Saitoh M, Tanimura A
  • Journal Title: Biomedical Research Volume: 37 Issue: 6 Pages: 329-334
  • DOI: 10.2220/biomedres.37.329
  • NAID: 130005252683
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed
• [Journal Article] Globoside accelerates the differentiation of dental epithelial cells into ameloblasts (2016)
  • Author(s): Nakamura, T., Chiba, Y., Naruse, M., Harada, H., and Fukumoto, S.
  • Journal Title: Intenational Journal of Oral Science Volume: 8(4) Issue: 4 Pages: 205-212
  • DOI: 10.1038/ijos.2016.35
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Plakophilin-1, a novel Wnt signaling regulator, is critical for tooth development and ameloblast differentiation (2016)
  • Author(s): Miyazaki K, Yoshizaki K, Arai C, Yamada A, Saito K, Ishikawa M, Xue H, Funada K, Haruyama N, Yamada Y, Fukumoto S, Takahashi I
  • Journal Title: Plos One Volume: 11 Issue: 3 Pages: 0152206-0152206
  • DOI: 10.1371/journal.pone.0152206
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ameloblastin, an Extracellular Matrix Protein, Affects Long Bone Growth and Mineralization. (2016)
  • Author(s): Lu X, Fukumoto S, Yamada Y, Evans CA, Diekwisch TG, Luan X.
  • Journal Title: J Bone Miner Res. Volume: in press Issue: 6 Pages: 1235-1246
  • DOI: 10.1002/jbmr.2788
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Pannexin 3 and connexin 43 modulate skeletal development through their distinct functions and expression patterns. (2016)
  • Author(s): Ishikawa M, Williams GL, Ikeuchi T, Sakai K, Fukumoto S, Yamada Y.
  • Journal Title: J Cell Sci. Volume: 129 Issue: 5 Pages: 1018-1030
  • DOI: 10.1242/jcs.176883
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1/5/8 phosphorylation (2016)
  • Author(s): Liu J, Saito K*, Maruya Y, Nakamura T, Yamada A, Fukumoto E, Ishikawa M, Iwamoto T, Miyazaki K, Yoshizaki K, Ge L, Fukumoto S
  • Journal Title: Scientific reports Volume: 6 Issue: 1 Pages: 23670-23670
  • DOI: 10.1038/srep23670
  • NAID: 120006534957
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Interaction between Fibronectin and β1 Integrin is Essential for Tooth Development. (2015)
  • Author(s): Saito, K., Fukumoto, E., Yamada, A., Yuasa, K., Yoshizaki, K., Iwamoto, T., Saito, M., Nakamura, T., and Fukumoto, S.
  • Journal Title: PLOS one Volume: 10(4) Issue: 4 Pages: 1-12
  • DOI: 10.1371/journal.pone.0121667
  • NAID: 120006872347
  • Peer Reviewed / Open Access
• [Journal Article] Novel Hedgehog Agonists Promote Osteoblast Differentiation in Mesenchymal Stem Cells (2015)
  • Author(s): Takashi Nakamura, Masahiro Naruse, Yuta Chiba, Toshihisa Komori, Keiichi Sasaki, Masahiro Iwamoto, Satoshi Fukumoto
  • Journal Title: Journal of Cellular Physiology Volume: 230(4) Issue: 4 Pages: 922-929
  • DOI: 10.1002/jcp.24823
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Epiprofin orchestrates epidermal keratinocyte proliferation and differentiation. (2014)
  • Author(s): Nakamura, T., Yoshitomi, Y., Patel, V., Fukumoto, S., and Yamada, Y.
  • Journal Title: Journal of cell science Volume: 127(24) Pages: 5261-5272
  • DOI: 10.1242/jcs.156778
  • Peer Reviewed / Open Access / Acknowledgement Compliant