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Regulation of epithelial polarization to regenerate tooth

Research Project

Project/Area Number 26670880
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Research InstitutionTohoku University

Principal Investigator

FUKUMOTO SATOSHI  東北大学, 歯学研究科, 教授 (30264253)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsエナメル質 / 3次元培養 / 石灰化 / 歯の再生 / 上皮ー間葉相互作用
Outline of Final Research Achievements

Enamel formation is known as a process that involves cellular proliferation, differentiation through sequential epithelial-mesenchymal interactions, secretion of tissue-specific matrix proteins (e.g., amelogenin), active conveyance of inorganic ions including calcium, and the onset of precipitation and orientation of enamel apatite cystals with enamel matrices. To form artificial enamel, in vitro differentiation system of ameloblast is useful to regenerate tooth and hard tissue. Here, we established new 3D culture system to induce ameloblast differentiation and calcification. A novel 3D culture for dental epithelial cell line SF2 induced enamel specific extracellular matrix, a meloblastin and amelogenin. Further, calcification was observed in this culture system indicating that this new culture method might be useful to form artificial enamel in vitro.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report

Research Products

(11 results)

All 2017 2016 2015 2014

All Journal Article

  • [Journal Article] Nephronectin plays critical roles in Sox2 expression and proliferation in dental epithelial stem cells via EGF-like repeat domains2017

    • Author(s)
      Arai C, Yoshizaki K, Miyazaki K, Saito K, Yamada A, Han X, Funada K, Fukumoto E, Haruyama N, Iwamoto T, Takahashi I, Fukumoto S.
    • Journal Title

      Scientific reports

      Volume: 27 Pages: 45181-45181

    • DOI

      10.1038/srep45181

    • NAID

      120006534986

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Practical whole-tooth regenerative therapy utilizing autologous bioengineered tooth germ transplantation in a post-natal canine model.2017

    • Author(s)
      Ono M, Oshima M, Ogawa M, Sonoyama W, Hara ES, Oida Y, Shinkawa S, Nakajima R , Mine A ,Hayano S, Fukumoto S , Kasugai8 S , Yamaguchi A, Tsuji T, Kuboki T
    • Journal Title

      Scientific reports

      Volume: 7 Pages: 1-11

    • DOI

      10.1038/srep44522

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] <b>Effect of 1,25-dihydroxyvitamin D</b><b><sub>3</sub></b><b> on spontaneous calcium responses in rat dental epithelial SF2 cells revealed by long-term </b><b>imaging </b>2016

    • Author(s)
      Murata K, Takahashi A, Morita T, Nezu A, Fukumoto S, Saitoh M, Tanimura A
    • Journal Title

      Biomed. Res.

      Volume: 37 Issue: 6 Pages: 329-334

    • DOI

      10.2220/biomedres.37.329

    • NAID

      130005252683

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Globoside accelerates the differentiation of dental epithelial cells into ameloblasts.2016

    • Author(s)
      Nakamura, T., Chiba, Y., Naruse, M., Harada, H., and Fukumoto, S.
    • Journal Title

      Int J Oral Sci.

      Volume: 8(4) Pages: 205-205

    • DOI

      10.1038/ijos.2016.35

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Plakophilin-1, a novel Wnt signaling regulator, is critical for tooth development and ameloblast differentiation.2016

    • Author(s)
      Miyazaki K, Yoshizaki K, Arai C, Yamada A, Saito K, Ishikawa M, Xue H, Funada K, Haruyama N, Yamada Y, Fukumoto S, Takahashi I
    • Journal Title

      PLoS One

      Volume: 11 Pages: 0152206-0152206

    • DOI

      10.1371/journal.pone.0152206

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Ameloblastin, an Extracellular Matrix Protein, Affects Long Bone Growth and Mineralization.2016

    • Author(s)
      Lu X, Fukumoto S, Yamada Y, Evans CA, Diekwisch TG, Luan X.
    • Journal Title

      J Bone Miner Res.

      Volume: in press

    • DOI

      10.1002/jbmr.2788

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Pannexin 3 and Connexin43 Modulate Skeletal Development via Distinct Functions and Expression Patterns2016

    • Author(s)
      Ishikawa M, Williams GL, Ikeuchi T, Sakai K, Fukumoto S, Yamada Y.
    • Journal Title

      J Cell Sci.

      Volume: 129 Pages: 1018-1030

    • DOI

      10.1242/jcs.176883

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1/5/8 phosphorylation.2016

    • Author(s)
      Liu J, Saito K*, Maruya Y, Nakamura T, Yamada A, Fukumoto E, Ishikawa M, Iwamoto T, Miyazaki K, Yoshizaki K, Ge L, Fukumoto S
    • Journal Title

      Sci Rep.

      Volume: 6 Pages: 23670-23670

    • DOI

      10.1038/srep23670

    • NAID

      120006534957

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Interaction between fibronectin and β1 integrin is essential for tooth development.2015

    • Author(s)
      Saito, K., Fukumoto, E., Yamada, A., Yuasa, K., Yoshizaki, K., Iwamoto, T., Saito, M., Nakamura, T., and Fukumoto, S.
    • Journal Title

      PLoS ONE

      Volume: 10(4) Pages: 1-12

    • DOI

      10.1371/journal.pone.0121667

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Novel Hedgehog Agonists Promote Osteoblast Differentiation in Mesenchymal Stem Cells.2015

    • Author(s)
      Takashi Nakamura, Masahiro Naruse, Yuta Chiba, Toshihisa Komori, Keiichi Sasaki, Masahiro Iwamoto, Satoshi Fukumoto
    • Journal Title

      J Cell Physiol.

      Volume: 230(4) Pages: 9220929-9220929

    • DOI

      10.1002/jcp.24823

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Epiprofin orchestrates epidermal keratinocyte proliferation and differentiation2014

    • Author(s)
      Nakamura, T., Yoshitomi, Y., Patel, V., Fukumoto, S., and Yamada, Y.
    • Journal Title

      J Cell Sci.

      Volume: 127(24) Pages: 5261-5272

    • DOI

      10.1242/jcs.156778

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant

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Published: 2014-04-04   Modified: 2018-03-22  

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