Regulation of epithelial polarization to regenerate tooth
| Project/Area Number | 26670880 |
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed(Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost : ¥2,800,000、Indirect Cost : ¥840,000)
Fiscal Year 2016 : ¥780,000 (Direct Cost : ¥600,000、Indirect Cost : ¥180,000)
Fiscal Year 2015 : ¥780,000 (Direct Cost : ¥600,000、Indirect Cost : ¥180,000)
Fiscal Year 2014 : ¥2,080,000 (Direct Cost : ¥1,600,000、Indirect Cost : ¥480,000)
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| Keywords | エナメル質 / ３次元培養 / 石灰化 / 歯の再生 / 上皮ー間葉相互作用 |
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| Outline of Final Research Achievements |
Enamel formation is known as a process that involves cellular proliferation, differentiation through sequential epithelial-mesenchymal interactions, secretion of tissue-specific matrix proteins (e.g., amelogenin), active conveyance of inorganic ions including calcium, and the onset of precipitation and orientation of enamel apatite cystals with enamel matrices. To form artificial enamel, in vitro differentiation system of ameloblast is useful to regenerate tooth and hard tissue. Here, we established new 3D culture system to induce ameloblast differentiation and calcification. A novel 3D culture for dental epithelial cell line SF2 induced enamel specific extracellular matrix, a meloblastin and amelogenin. Further, calcification was observed in this culture system indicating that this new culture method might be useful to form artificial enamel in vitro.
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Report
(4results)
Research Products
(11results)
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[Journal Article] Nephronectin plays critical roles in Sox2 expression and proliferation in dental epithelial stem cells via EGF-like repeat domains2017
Author(s)
Arai C, Yoshizaki K, Miyazaki K, Saito K, Yamada A, Han X, Funada K, Fukumoto E, Haruyama N, Iwamoto T, Takahashi I, Fukumoto S.
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Journal Title
Scientific reports
Volume : 27
Pages : 45181-45181
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model.2017
Author(s)
Ono M, Oshima M, Ogawa M, Sonoyama W, Hara ES, Oida Y, Shinkawa S, Nakajima R , Mine A ,Hayano S, Fukumoto S , Kasugai8 S , Yamaguchi A, Tsuji T, Kuboki T
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Journal Title
Scientific reports
Volume : 7
Pages : 1-11
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Plakophilin-1, a novel Wnt signaling regulator, is critical for tooth development and ameloblast differentiation2016
Author(s)
Miyazaki K, Yoshizaki K, Arai C, Yamada A, Saito K, Ishikawa M, Xue H, Funada K, Haruyama N, Yamada Y, Fukumoto S, Takahashi I
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Journal Title
Plos One
Volume : 11
Pages : 0152206-0152206
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1/5/8 phosphorylation2016
Author(s)
Liu J, Saito K*, Maruya Y, Nakamura T, Yamada A, Fukumoto E, Ishikawa M, Iwamoto T, Miyazaki K, Yoshizaki K, Ge L, Fukumoto S
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Journal Title
Sci Rep.
Volume : 6
Pages : 23670-23670
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Interaction between Fibronectin and β1 Integrin is Essential for Tooth Development.2015
Author(s)
Saito, K., Fukumoto, E., Yamada, A., Yuasa, K., Yoshizaki, K., Iwamoto, T., Saito, M., Nakamura, T., and Fukumoto, S.
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Journal Title
PLOS one
Volume : 10(4)
Pages : 1-12
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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