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Development of xeno-free human feeder cell with multi-function derived from the dental pulp cell

Research Project

Project/Area Number 26670883
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Research InstitutionNiigata University

Principal Investigator

Issei Saitoh  新潟大学, 医歯学系, 准教授 (90404540)

Co-Investigator(Kenkyū-buntansha) 佐藤 正宏  鹿児島大学, 医療ミニブタ先端医療開発研究センター, 教授 (30287099)
稲田 絵美  鹿児島大学, 医歯学域附属病院, 助教 (30448568)
野口 洋文  琉球大学, 医学(系)研究科(研究院), 教授 (50378733)
松山 清  久留米工業高等専門学校, 生物応用化学科, 准教授 (40299540)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords再生歯学 / 乳歯歯髄細胞 / iPS細胞 / feeder細胞
Outline of Final Research Achievements

Feeder cell was necessary for the establishment of iPS cell. When we consider the clinical applicability of human iPS cell, we must use xeno-free feeder cell without mixture of a mouse origin cell.
The aim of this study was the development of xeno-free human feeder cell with multi-function derived from the dental pulp cell. The maintenance of the iPS cell established from human deciduous dental pulp cells (HDDPCs) was possible as the feeder cell, but it was difficult to establish iPS cell from HDDPCs using the feeder cell. It was considered that the number of passages of the primary cell and the influence by the quality of HDDPCs of the cultivate condition.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (11 results)

All 2017 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Journal Article] Isolation and characterization of lymphoid enhancer factor-1-positive deciduous dental pulp stem-like cells after transfection with a piggyBac vector containing LEF1 promoter-driven selection markers2017

    • Author(s)
      Tomoya Murakami, Issei Saitoh, Masahiro Sato, Emi Inada, Miki Soda, Masataka Oda, Hisanori Domone, Yoko Iwase, Tadashi Sawami, Kazunari Matsueda, Yutaka Terao, Hayato Ohshima, Hirofumi Noguchi, Haruaki Hayasaki
    • Journal Title

      Archives of Oral Biology

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Efficient CRISPR/Cas9-based gene correction in induced pluripotent stem cells established from fibroblasts of patients with sickle cell disease2016

    • Author(s)
      Masahiro Sato, Issei Saitoh, Emi Inada
    • Journal Title

      Stem Cell Investigation

      Volume: 3 Pages: 78-78

    • DOI

      10.21037/sci.2016.11.05

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A combination of targeted toxin technology and the piggyBac-mediated gene transfer system enables efficient isolation of stable transfectants in nonhuman mammalian cells2015

    • Author(s)
      Sato M, Inada E, Saitoh I, Matsumoto Y, Ohtsuka M, Miura H, Nakamura S, Sakurai T, Watanabe S
    • Journal Title

      Biotechnolgy Journal

      Volume: 10 Issue: 1 Pages: 143-153

    • DOI

      10.1002/biot.201400283

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 遺伝子工学的手法による不死化ヒト乳歯歯髄細胞株の樹立と特性解析2017

    • Author(s)
      稲田絵美,齊藤一誠,窪田直子,村上智哉,左右田美樹,澤味 規,松枝一成,早崎治明,山崎要一
    • Organizer
      第55回日本小児歯科学会大会
    • Place of Presentation
      西日本総合展示場(福岡県北九州市)
    • Year and Date
      2017-05-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウスすい臓内へのpiggyBac系を介した直接生体内遺伝子導入は外来遺伝子の長期発現を可能とする2016

    • Author(s)
      佐藤正宏,稲田絵美,齋藤一誠,三浦浩美,大塚正人,中村伸吾,桜井敬之,渡部聡
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] The genetic engineering-based isolation of lymphoid enhancer-binding factor-1 (LEF1) positive stem-like cells from human deciduous tooth cell-derived iPSCs2016

    • Author(s)
      Tomoya Murakami, Issei Saitoh, Masahiro Sato, Emi Inada, Miki Soda, Yoko Iwase, Tadashi Sawami, Ayako Suzuki, Hayato Ohshima, Haruaki hayasaki
    • Organizer
      10th Biennial Conference of the Pediatric Dentistry Association of Asia in conjunction with 54th Annual Conference of the Japanese Society of Pediatric Dentistry
    • Place of Presentation
      東京ドームホテル(東京都文京区)
    • Year and Date
      2016-05-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒト乳歯歯髄細胞のアルカリホスファターゼ活性とOCT-3/4発現はiPS細胞樹立の可否を予測する有効なマーカーである2015

    • Author(s)
      稲田絵美,佐藤正宏,齊藤一誠,窪田直子,澤味 規 ,村上智哉,左右田美樹,早崎治明,山﨑要一
    • Organizer
      第53回日本小児歯科学会大会
    • Place of Presentation
      広島市広島国際会議場
    • Year and Date
      2015-05-21 – 2015-05-22
    • Related Report
      2014 Research-status Report
  • [Presentation] piggyBac-transposon-mediated gene-delivery efficiently generates stable transfectants from HDDPCs and HDDPC-derived-iPSCs2015

    • Author(s)
      Miki Soda, Issei Saitoh, Emi Inada, Tomoya Murakami, Yoko Iwase, Naoko Kubota, Tadashi Sawami, Yuko Matsumoto, Youichi Yamasaki, Haruaki Hayasaki, Hayato Ohshima, Masahiro Sato
    • Organizer
      IADR/AADR/CADR 93rd General Session and Exhibition
    • Place of Presentation
      Boston
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Research-status Report
  • [Presentation] piggyBacトランスポゾンによるヒト乳歯歯髄細胞からの遺伝子導入安定株の効率的取得2014

    • Author(s)
      稲田絵美,齊藤一誠,窪田直子,松本裕子,村上智哉,澤味規,山崎要一
    • Organizer
      第52回日本小児歯科学会大会
    • Place of Presentation
      東京都品川区きゅりあん
    • Year and Date
      2014-05-16 – 2014-05-17
    • Related Report
      2014 Research-status Report
  • [Remarks] 新潟大学 大学院医歯学総合研究科 小児歯科学分野

    • URL

      http://www.dent.niigata-u.ac.jp/pedo/pedo.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 新潟大学大学院医歯学総合研究科 小児歯科学分野

    • URL

      http://www.dent.niigata-u.ac.jp/pedo/pedo.html

    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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