Development of xeno-free human feeder cell with multi-function derived from the dental pulp cell
| Project/Area Number |
26670883
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
Issei Saitoh 新潟大学, 医歯学系, 准教授 (90404540)
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 正宏 鹿児島大学, 医療ミニブタ先端医療開発研究センター, 教授 (30287099)
稲田 絵美 鹿児島大学, 医歯学域附属病院, 助教 (30448568)
野口 洋文 琉球大学, 医学(系)研究科(研究院), 教授 (50378733)
松山 清 久留米工業高等専門学校, 生物応用化学科, 准教授 (40299540)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 再生歯学 / 乳歯歯髄細胞 / iPS細胞 / feeder細胞 |
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| Outline of Final Research Achievements |
Feeder cell was necessary for the establishment of iPS cell. When we consider the clinical applicability of human iPS cell, we must use xeno-free feeder cell without mixture of a mouse origin cell.
The aim of this study was the development of xeno-free human feeder cell with multi-function derived from the dental pulp cell. The maintenance of the iPS cell established from human deciduous dental pulp cells (HDDPCs) was possible as the feeder cell, but it was difficult to establish iPS cell from HDDPCs using the feeder cell. It was considered that the number of passages of the primary cell and the influence by the quality of HDDPCs of the cultivate condition.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Isolation and characterization of lymphoid enhancer factor-1-positive deciduous dental pulp stem-like cells after transfection with a piggyBac vector containing LEF1 promoter-driven selection markers2017
Author(s)
Tomoya Murakami, Issei Saitoh, Masahiro Sato, Emi Inada, Miki Soda, Masataka Oda, Hisanori Domone, Yoko Iwase, Tadashi Sawami, Kazunari Matsueda, Yutaka Terao, Hayato Ohshima, Hirofumi Noguchi, Haruaki Hayasaki
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Journal Title
Archives of Oral Biology
Volume: 印刷中
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] piggyBac-transposon-mediated gene-delivery efficiently generates stable transfectants from HDDPCs and HDDPC-derived-iPSCs2015
Author(s)
Miki Soda, Issei Saitoh, Emi Inada, Tomoya Murakami, Yoko Iwase, Naoko Kubota, Tadashi Sawami, Yuko Matsumoto, Youichi Yamasaki, Haruaki Hayasaki, Hayato Ohshima, Masahiro Sato
Organizer
IADR/AADR/CADR 93rd General Session and Exhibition
Place of Presentation
Boston
Year and Date
2015-03-11 – 2015-03-14
Related Report
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