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Identification for "tooth genes" in neural crest derived mesenchmal cells

Research Project

Project/Area Number 26670886
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Research InstitutionKyushu University

Principal Investigator

Yoshizaki Keigo  九州大学, 歯学研究院, 助教 (10507982)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords歯 / 再生医学 / 細胞分化 / 間葉細胞 / 細胞凝集 / 歯原性上皮細胞
Outline of Final Research Achievements

Tooth morphogenesis is initiated by epithelial-mesenchymal interaction, and the neural crest-derived mesenchymal cells play an important role in tooth development. In this study, we identified two genes, which were specifically expressed in mesenchymal cells in tooth development. These genes were strongly expressed in E13-E16 tooth germ, which is the morphogenesis stage of tooth development, and the expression level was declined in the differentiation stage. Furthermore, we identified that dental mesenchymal cells transfected with identified genes were critical for epithelial-mesenchymal interaction. These results suggest that the identified genes may play important roles in tooth morphogenesis.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (21 results)

All 2017 2016 2015 2014

All Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 3 results) Presentation (14 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results)

  • [Journal Article] Nephronectin plays critical roles in Sox2 expression and proliferation in dental epithelial stem cells via EGF-like repeat domains2017

    • Author(s)
      Chieko Arai, Keigo Yoshizaki, Kanako Miyazaki, Kan Saito, Aya Yamada, Xue Han, Keita Funada, Emiko Fukumoto, Naoto Haruyama, Tsutomu Iwamoto, Ichiro Takahashi, Satoshi Fukumoto
    • Journal Title

      Scientific Reports

      Volume: 27 Pages: 45181-45181

    • NAID

      120006534986

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Plakophilin-1, a novel Wnt signaling regulator, is critical for tooth development and ameloblast differentiation2016

    • Author(s)
      Miyazaki K, Yoshizaki K, Arai C, Yamada A, Saito K, Ishikawa M, Xue H, Funada K, Haruyama N, Yamada Y, Fukumoto S, Takahashi I
    • Journal Title

      Plos One

      Volume: 11 Issue: 3 Pages: 0152206-0152206

    • DOI

      10.1371/journal.pone.0152206

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Connexin 43 is necessary for salivary gland branching morphogenesis and FGF10-induced ERK1/2 phosphorylation2016

    • Author(s)
      Yamada A, Futagi M, Fukumoto E, Saito K, Yoshizaki K, Ishikawa M, Arakaki M, Hino R, Sugawara Y, Ishikawa M, Naruse M, Miyazaki K, Nakamura T, Fukumoto S.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 291 Issue: 2 Pages: 904-912

    • DOI

      10.1074/jbc.m115.674663

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1/5/8 phosphorylation2016

    • Author(s)
      Liu J, Saito K*, Maruya Y, Nakamura T, Yamada A, Fukumoto E, Ishikawa M, Iwamoto T, Miyazaki K, Yoshizaki K, Ge L, Fukumoto S
    • Journal Title

      Scientific reports

      Volume: 6 Issue: 1 Pages: 23670-23670

    • DOI

      10.1038/srep23670

    • NAID

      120006534957

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Interaction between Fibronectin and β1 Integrin is Essential for Tooth Development.2015

    • Author(s)
      Saito, K., Fukumoto, E., Yamada, A., Yuasa, K., Yoshizaki, K., Iwamoto, T., Saito, M., Nakamura, T., and Fukumoto, S.
    • Journal Title

      PLOS one

      Volume: 10(4) Issue: 4 Pages: 1-12

    • DOI

      10.1371/journal.pone.0121667

    • NAID

      120006872347

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] MicroRNA-200a Regulates the Development of Mandibular Condylar Cartilage2015

    • Author(s)
      M. Umeda
    • Journal Title

      Journal of dental research

      Volume: 95 Issue: 6 Pages: 795-802

    • DOI

      10.1177/0022034515577411

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair.2014

    • Author(s)
      Yoshizaki K, Hu L, Nguyen T, Sakai K, He B, Fong C, Yamada Y, Bikle DD, Oda Y.
    • Journal Title

      Plos One

      Volume: 9 Issue: 6 Pages: e99991-e99991

    • DOI

      10.1371/journal.pone.0099991

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] PKP1 regulates ameloblast differentiation via the cell adhesion2017

    • Author(s)
      Kanako Miyazaki, Keigo Yoshizaki, Chieko Arai, Xue Han, Keita Funada, Naoto Haruyama, Satoshi Fukumoto, Ichiro Takahashi
    • Organizer
      The IADR/AADR/CADR 95th General Session and Exhibition
    • Place of Presentation
      San Francisco, CA, USA
    • Year and Date
      2017-03-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Epiprofin and T-box1 Regulate Ameloblast Linage Development2017

    • Author(s)
      Yuta Chiba, Kiyoshi Sakai, Tomoko Ikeuchi, Keigo Yoshizaki, Darius Mahboubi, Bing He, Yoshihiko Yamada
    • Organizer
      The IADR/AADR/CADR 95th General Session and Exhibition
    • Place of Presentation
      San Francisco, CA, USA
    • Year and Date
      2017-03-22
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Stim1はマウスのエナメル質の石灰化およびエナメル芽細胞成熟期にみられる周期性を制御する2017

    • Author(s)
      古川 雄亮、春山 直人、二階堂 まりこ、中西 正光、笠 法子、大洞 將嗣、呉本 晃一、吉崎 恵悟、高橋 一郎
    • Organizer
      第12回九州矯正歯科学会学術大会
    • Place of Presentation
      宮崎
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] ストア作動性Ca2+流入異常による外胚葉異形成症におけるエナメル質形成不全症の発症メカニズム2016

    • Author(s)
      古川 雄亮、春山 直人、二階堂 まりこ、中西 正光、笠 法子、大洞 將嗣、呉本 晃一 吉崎 恵悟、高橋 一郎
    • Organizer
      第75回日本矯正歯科学会
    • Place of Presentation
      徳島
    • Year and Date
      2016-11-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] 基底膜分子Nephronectin はEGF like repeat domain を介して歯原性上皮幹細胞の細胞増殖を制御する2016

    • Author(s)
      新井智映子,吉崎恵悟,宮崎佳奈子,韓雪,鮒田啓太,福本敏,高橋一郎
    • Organizer
      第58回歯科基礎医学会学術大会・総会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 歯原性上皮細胞のエナメル芽細胞への分化におけるEpiprofin とT-box1の役割2016

    • Author(s)
      酒井陽,吉崎恵悟,千葉雄太,池内友子,山本朗仁,日比英晴,山田吉彦
    • Organizer
      第58回歯科基礎医学会学術大会・総会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2016-08-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Comprehensive analysis for the functions of genes during epithelial-mesenchymal interactions in tissue morphogenesis2016

    • Author(s)
      Keigo Yoshizaki
    • Organizer
      「口腔から健康長寿を支えるプロジェクト推進に向けた研究拠点構築プログラム」2nd Symposium
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2016-02-27
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Plakophilin-1, a novel Wnt signaling regulator, is critical for tooth development and ameloblast differentiation.2015

    • Author(s)
      Kanako Miyazaki, Keigo Yoshizaki, Chieko Arai, Han Xue, Keita Funada, Ichiro Takahashi
    • Organizer
      International Symposium 2015 Oral and Craniofacial Development and Disease
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2015-12-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 外胚葉異形成症原因遺伝子Plakophilin 1は歯の発生においてWntシグナル調節因子として作用する2015

    • Author(s)
      宮崎佳奈子、吉崎恵悟、新井智映子、韓雪、鮒田啓太、高橋一郎
    • Organizer
      第74回日本矯正歯科学会
    • Place of Presentation
      福岡市
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
  • [Presentation] 歯の発生における基底膜分子Nephronectinの役割2015

    • Author(s)
      新井智映子、吉崎恵悟、宮崎佳奈子、韓雪、鮒田啓太、高橋一郎
    • Organizer
      第74回日本矯正歯科学会
    • Place of Presentation
      福岡市
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
  • [Presentation] Plakophilin-1, a novel Wnt signaling regulator, is critical for tooth development and ameloblast differentiation2015

    • Author(s)
      宮崎佳奈子、吉崎恵悟、新井智映子、韓雪、鮒田啓太、高橋一郎
    • Organizer
      第57回歯科基礎医学会学術大会・総会
    • Place of Presentation
      新潟市
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] A basement membrane protein Nephronectin is involved in dental epithelial stem cell differentiation and proliferation.2015

    • Author(s)
      新井智映子、吉崎恵悟、宮崎佳奈子、韓雪、鮒田啓太、高橋一郎
    • Organizer
      第57回歯科基礎医学会学術大会・総会
    • Place of Presentation
      新潟市
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] 歯の発生における外胚葉異形成症原因遺伝子Plakophilin 1 (Pkp1)の役割2014

    • Author(s)
      宮崎佳奈子、吉崎恵悟、新井智映子、張麗麗、韓雪、春山直人、高橋一郎
    • Organizer
      第73回日本矯正歯科学会
    • Place of Presentation
      千葉市
    • Year and Date
      2014-10-20 – 2014-10-22
    • Related Report
      2014 Research-status Report
  • [Presentation] 歯原性上皮細胞におけるCa2+を介した細胞接着メカニズム及び分化に及ぼす影響について2014

    • Author(s)
      宮崎佳奈子、吉崎恵悟、新井智映子、張麗麗、韓雪、春山直人、高橋一郎
    • Organizer
      第56回歯科基礎医学会学術大会・総会
    • Place of Presentation
      福岡市
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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