| Project/Area Number |
26670887
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
寺尾 文恵 九州大学, 歯学研究院, 助教 (10510018)
安永 敦 九州大学, 歯学研究院, 共同研究員 (80515990)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 歯科矯正学 / オートファジー / 歯根膜 / 線維芽細胞 / アポトーシス / 低酸素 / 歯学 / 歯の移動 / メカニカルストレス / 細胞死 |
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| Outline of Final Research Achievements |
In this study, we examined the autophagy inducible factors in periodontal ligament (PDL), and tried to clarify the relationship between autophagy and apoptotic cell death. First, we moved the maxillary first molar of GFP-LC3 transgenic mice, and examined the localization of autophagy marker, LC3 in the PDL. Autophagy (LC3) was induced in the compression side of PDL at 6 hours after initiating the tooth movement. The autophagy was found in the hypoxic area induced by tooth movement. Furthermore, autophagy was induced prior to the PDL cell apoptosis. In order to clarify the autophagy inducible factors in the PDL cells, low oxygen partial pressure were loaded to a human PDL fibroblast cell line, and resulted in increased cellular LC3 protein levels. These results suggest that the orthodontic tooth movement induces autophagy on compression side of PDL, and hypoxia is one of the critical inducers of autophagy.
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