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The roles and regulatory factors of cellular ligistics, autophagy maintaining the homeostasis of periodontal ligament.

Research Project

Project/Area Number 26670887
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Research InstitutionKyushu University

Principal Investigator

HARUYAMA NAOTO  九州大学, 歯学研究院, 准教授 (70359529)

Co-Investigator(Kenkyū-buntansha) 寺尾 文恵  九州大学, 歯学研究院, 助教 (10510018)
安永 敦  九州大学, 歯学研究院, 共同研究員 (80515990)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords歯科矯正学 / オートファジー / 歯根膜 / 線維芽細胞 / アポトーシス / 低酸素 / 歯学 / 歯の移動 / メカニカルストレス / 細胞死
Outline of Final Research Achievements

In this study, we examined the autophagy inducible factors in periodontal ligament (PDL), and tried to clarify the relationship between autophagy and apoptotic cell death. First, we moved the maxillary first molar of GFP-LC3 transgenic mice, and examined the localization of autophagy marker, LC3 in the PDL. Autophagy (LC3) was induced in the compression side of PDL at 6 hours after initiating the tooth movement. The autophagy was found in the hypoxic area induced by tooth movement. Furthermore, autophagy was induced prior to the PDL cell apoptosis. In order to clarify the autophagy inducible factors in the PDL cells, low oxygen partial pressure were loaded to a human PDL fibroblast cell line, and resulted in increased cellular LC3 protein levels. These results suggest that the orthodontic tooth movement induces autophagy on compression side of PDL, and hypoxia is one of the critical inducers of autophagy.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2017 2016 2015

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (2 results)

  • [Journal Article] Nephronectin plays critical roles in Sox2 expression and proliferation in dental epithelial stem cells via EGF-like repeat domains.2017

    • Author(s)
      Arai C, Yoshizaki K, Miyazaki K, Saito K, Yamada A, Han X, Funada K, Fukumoto E, Haruyama N, Iwamoto T, Takahashi I, Fukumoto S.
    • Journal Title

      Sci Rep.

      Volume: 27 Issue: 1 Pages: 45181-45181

    • DOI

      10.1038/srep45181

    • NAID

      120006534986

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Clinicostatistical analysis of congenitally missing permanent teeth in Japanese patients with cleft lip and/or palate.2016

    • Author(s)
      Sato M, Baba Y, Haruyama N, Higashihori N, Tsuji M, Suzuki S, Moriyama K.
    • Journal Title

      Orthodontic Waves.

      Volume: 75 Issue: 2 Pages: 41-45

    • DOI

      10.1016/j.odw.2016.06.001

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 口唇裂口蓋裂患者の矯正歯科受診と咬合の実態 -九州大学病院矯正歯科における43年間の臨床統計-2016

    • Author(s)
      祐田京子, 丸山和宏, 鈴木陽, 安永敦, 下村卓弘, 吉崎恵悟, 春山直人, 高橋一郎
    • Journal Title

      日本口蓋裂学会雑誌

      Volume: 41 Pages: 192-200

    • NAID

      130005281889

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plakophilin-1, a novel Wnt signaling regulator, is critical for tooth development and ameloblast differentiation2016

    • Author(s)
      Miyazaki K, Yoshizaki K, Arai C, Yamada A, Saito K, Ishikawa M, Xue H, Funada K, Haruyama N, Yamada Y, Fukumoto S, Takahashi I
    • Journal Title

      Plos One

      Volume: 11 Issue: 3 Pages: 0152206-0152206

    • DOI

      10.1371/journal.pone.0152206

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Relationship between length variations in Ser/Asp-rich repeats in phosphophoryn and in vitro precipitation of calcium phosphate.2015

    • Author(s)
      Kobuke S, Suzuki S, Hoshino H, Haruyama N, Nishimura F, Shiba H.
    • Journal Title

      Arch Oral Biol

      Volume: 60 Issue: 9 Pages: 1263-72

    • DOI

      10.1016/j.archoralbio.2015.05.013

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Stim1はマウスのエナメル質の石灰化およびエナメル芽細胞成熟期にみられる周期性を制御する2017

    • Author(s)
      古川雄亮, 春山直人, 二階堂まりこ, 中西正光, 笠 法子, 大洞將嗣, 呉本晃一, 吉崎恵悟, 高橋一郎.
    • Organizer
      第12回九州矯正歯科学会学術大会
    • Place of Presentation
      宮崎
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
  • [Presentation] ストア作動性Ca2+流入の異常による外胚葉異形成症におけるエナメル質形成不全の発症メカニズム.2016

    • Author(s)
      古川雄亮, 春山直人, 二階堂まりこ, 中西正光, 笠 法子, 大洞將嗣, 呉本晃一, 吉崎恵悟, 高橋一郎
    • Organizer
      第75回 日本矯正歯科学会大会
    • Place of Presentation
      徳島
    • Year and Date
      2016-11-07
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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