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Diagnosis of craniofacial disorders with dental pulp stem cells in Down syndrome

Research Project

Project/Area Number 26670891
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Orthodontics/Pediatric dentistry
Research InstitutionKanagawa Children's Medical Center (Clinical Research Institute)

Principal Investigator

SASAKI YASUNORI  地方独立行政法人神奈川県立病院機構神奈川県立こども医療センター(臨床研究所), その他部局等, その他 (70332848)

Co-Investigator(Kenkyū-buntansha) SHIMBO Hiroko  神奈川県立こども医療センター, 臨床研究所, 研究員 (50724663)
TAYA Yuji  日本歯科大学, 病理学教室, 准教授 (30197587)
Co-Investigator(Renkei-kenkyūsha) SAKUMA Syuji  神奈川歯科大学横浜クリニック, 矯正歯科, 医員 (00535429)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsダウン症 / 上顎骨劣成長 / 歯髄幹細胞 / 骨芽細胞分化 / mRNA / 顎顔面頭蓋 / 骨芽細胞 / 発生・分化 / 骨成長 / mRNA / Ⅰ型コラーゲン / オステオネクチン
Outline of Final Research Achievements

We investigated the signaling molecules regarding osteoblast growth and differentiation using dental pulp cells of Down syndrome patients with maxillary retrogression. The dental pulp cells were derived from primary tooth of two patients with Down syndrome and control individuals, respectively. The RNA samples were isolated from cultured cells. The mRNA expression levels of genes on chromosome21 and the developmental stages of osteoblast were quantified by real-time RT-PCR.As the results, in Down syndrome samples, expression levels of USP16 and DYRK1A on chromosome21 were upregulated at 1.5 fold. In addition, Collagen typeI genes and Osteonectin were significantly upregulated in Down syndrome.
The upregulation of Collagen typeI genes and Osteonectin in Down syndrome was a reverse phenomenon to explain the defection of maxillary growth of Down syndrome. The environmental factors such protrusion of tongue might work as the important ones in the defect of maxillary growth in the syndrome.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2016 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 2 results) Presentation (3 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] ダウン症における歯列咬合治療との発達の関連性についての研究2015

    • Author(s)
      佐々木康成、佐久間秀二
    • Journal Title

      こども医療センター医学誌

      Volume: 44 Pages: 271-271

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Noonan症候群に伴う摂食嚥下障害の1症例2015

    • Author(s)
      高橋 摩理, 弘中 祥司、久保寺 友子, 佐々木 康成
    • Journal Title

      日本障害者歯科学会雑誌

      Volume: 36 Pages: 118-123

    • NAID

      130005106210

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Myhre Syndrome: A Rare Craniofacial Disorder.2014

    • Author(s)
      Ishibashi N, Sasaki Y, Asakura Y.
    • Journal Title

      Journal of Craniomandibular Practice

      Volume: 32 Pages: 300-6

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Molecular contribution to cleft palate production in cleft lip mice,2014

    • Author(s)
      Sasaki, Y., Taya, Y., Saito, K., Fujita, K., Aoba, T. and Fujiwara, T.
    • Journal Title

      Congenital Anomalies

      Volume: 54: Pages: 94-99

    • NAID

      120006986612

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Diagnosis of osteoblast differentiation with dental pulp stem cells in Down syndrome2016

    • Author(s)
      Sasaki Y, Shimbo H, Taya Y
    • Organizer
      10th Biennial Conference of the Pediatric Dentistry Association of Asia
    • Place of Presentation
      Tokyo Dome Hotel (Bunkyo-ku、Tokyo)
    • Year and Date
      2016-05-26
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 魚鱗症候群児の歯科所見,2015

    • Author(s)
      久保寺友子、大江智可子、佐久間秀二、佐々木康成
    • Organizer
      第32回日本障害者歯科学会総会および学術大会
    • Place of Presentation
      名古屋国際会議場(愛知県名古屋市熱田区)
    • Year and Date
      2015-11-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] Regulation of osteoblast derived from dental pulp cells of cleidocranial. .2014

    • Author(s)
      Sasaki, Y, Shimbo,H, Osaka, H
    • Organizer
      The9th Congress of Asian Association of Paediatric Dentistry
    • Place of Presentation
      シンガポール
    • Year and Date
      2014-08-22 – 2014-08-24
    • Related Report
      2014 Research-status Report
  • [Book] 歯界展望2015

    • Author(s)
      佐々木 康成(共著)
    • Publisher
      デンタルダイヤモンド社
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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