Diagnosis of craniofacial disorders with dental pulp stem cells in Down syndrome

• Project/Area Number: 26670891
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Kanagawa Children's Medical Center (Clinical Research Institute)
• Principal Investigator: SASAKI YASUNORI 地方独立行政法人神奈川県立病院機構神奈川県立こども医療センター(臨床研究所), その他部局等, その他 (70332848)
• Co-Investigator(Kenkyū-buntansha): SHIMBO Hiroko 神奈川県立こども医療センター, 臨床研究所, 研究員 (50724663) ; TAYA Yuji 日本歯科大学, 病理学教室, 准教授 (30197587)
• Co-Investigator(Renkei-kenkyūsha): SAKUMA Syuji 神奈川歯科大学横浜クリニック, 矯正歯科, 医員 (00535429)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ダウン症 / 上顎骨劣成長 / 歯髄幹細胞 / 骨芽細胞分化 / mRNA / 顎顔面頭蓋 / 骨芽細胞 / 発生・分化 / 骨成長 / ｍＲＮＡ / Ⅰ型コラーゲン / オステオネクチン

Outline of Final Research Achievements

We investigated the signaling molecules regarding osteoblast growth and differentiation using dental pulp cells of Down syndrome patients with maxillary retrogression. The dental pulp cells were derived from primary tooth of two patients with Down syndrome and control individuals, respectively. The RNA samples were isolated from cultured cells. The mRNA expression levels of genes on chromosome21 and the developmental stages of osteoblast were quantified by real-time RT-PCR.As the results, in Down syndrome samples, expression levels of USP16 and DYRK1A on chromosome21 were upregulated at 1.5 fold. In addition, Collagen typeI genes and Osteonectin were significantly upregulated in Down syndrome.
The upregulation of Collagen typeI genes and Osteonectin in Down syndrome was a reverse phenomenon to explain the defection of maxillary growth of Down syndrome. The environmental factors such protrusion of tongue might work as the important ones in the defect of maxillary growth in the syndrome.

Research Products

• [Journal Article] ダウン症における歯列咬合治療との発達の関連性についての研究 (2015)
  • Author(s): 佐々木康成、佐久間秀二
  • Journal Title: こども医療センター医学誌 Volume: 44 Pages: 271-271
  • Peer Reviewed
• [Journal Article] Noonan症候群に伴う摂食嚥下障害の1症例 (2015)
  • Author(s): 高橋 摩理, 弘中 祥司、久保寺 友子, 佐々木 康成
  • Journal Title: 日本障害者歯科学会雑誌 Volume: 36 Pages: 118-123
  • NAID: 130005106210
  • Peer Reviewed
• [Journal Article] Myhre Syndrome: A Rare Craniofacial Disorder. (2014)
  • Author(s): Ishibashi N, Sasaki Y, Asakura Y.
  • Journal Title: Journal of Craniomandibular Practice Volume: 32 Pages: 300-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular contribution to cleft palate production in cleft lip mice, (2014)
  • Author(s): Sasaki, Y., Taya, Y., Saito, K., Fujita, K., Aoba, T. and Fujiwara, T.
  • Journal Title: Congenital Anomalies Volume: 54: Pages: 94-99
  • NAID: 120006986612
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Diagnosis of osteoblast differentiation with dental pulp stem cells in Down syndrome (2016)
  • Author(s): Sasaki Y, Shimbo H, Taya Y
  • Organizer: 10th Biennial Conference of the Pediatric Dentistry Association of Asia
  • Place of Presentation: Tokyo Ｄｏｍｅ Ｈｏｔｅｌ （Ｂｕｎｋｙｏ－ｋｕ、Ｔｏｋｙｏ）
  • Year and Date: 2016-05-26
  • Int'l Joint Research
• [Presentation] 魚鱗症候群児の歯科所見, (2015)
  • Author(s): 久保寺友子、大江智可子、佐久間秀二、佐々木康成
  • Organizer: 第32回日本障害者歯科学会総会および学術大会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市熱田区）
  • Year and Date: 2015-11-06
• [Presentation] Regulation of osteoblast derived from dental pulp cells of cleidocranial. . (2014)
  • Author(s): Sasaki, Y, Shimbo,H, Osaka, H
  • Organizer: The9th Congress of Asian Association of Paediatric Dentistry
  • Place of Presentation: シンガポール
  • Year and Date: 2014-08-22 – 2014-08-24
• [Book] 歯界展望 (2015)
  • Author(s): 佐々木 康成（共著）
  • Publisher: デンタルダイヤモンド社