| Project/Area Number |
26670894
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Periodontology
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| Research Institution | Meikai University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
友村 明人 明海大学, 歯学部, 教授 (60188810)
申 基てつ 明海大学, 歯学部, 教授 (40187555)
辰巳 順一 明海大学, 歯学部, 准教授 (60227105)
谷田部 一大 明海大学, 歯学部, 講師 (00526551)
大西 英知 明海大学, 歯学部, 助教 (30580279)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 間葉系細胞 / 顎骨 / 不織布フィルター / 再生療法 / 間葉系幹細胞 |
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| Outline of Final Research Achievements |
The purpose of this study was to evaluate the usefulness of a device with a nonwoven fabric filter, which selectively traps MSC based on affinity, for isolation of MSC from gnathic bone marrow. A total of 44 bone marrow aspirates were obtained from 35 patients during surgery for dental implant placement. The isolated cells were then seeded on tissue culture dishes. As a control, without using the device, the cells harvested by centrifugation were directly seeded on the dishes. As a result, colony forming cells by using the device were detected in 10 of 30 samples (33.3%). The colony forming cells without using the device were obtained in 3 of 14 samples (21.4%). It was thus suggested that the use of the device with a nonwoven fabric filter may allow efficient isolation of MSC from gnathic bone marrow in a closed system.
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