Comprehensive analysis of protein in gingival crevicular fluids from deciduous and permanent teeth using LC-ESI-MS/MS quantitative proteomics

• Project/Area Number: 26670896
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Periodontology
• Research Institution: Showa University
• Principal Investigator: YAMAMOTO Matsuo 昭和大学, 歯学部, 教授 (50332896)
• Co-Investigator(Kenkyū-buntansha): INOUE Mitsuko 昭和大学, 歯学部, 客員教授 (20112724) ; ITABE Hiroyuki 昭和大学, 薬学部, 教授 (30203079) ; KOIDE Yoko 昭和大学, 歯学部, 助教 (40407466)
• Project Period (FY): 2014-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 歯肉溝滲出液 / 乳歯 / 永久歯 / 好中球 / 質量分析 / 歯周炎 / 歯肉炎 / 混合歯列 / 網羅的解析

Outline of Final Research Achievements

To identify the biochemical characteristics of gingival tissues for distinguishing between permanent and deciduous teeth, the protein profiles of gingival crevicular fluids (GCF) were analyzed using quantitative proteomics. Forty children with mixed dentition (Hellman’s dental age IIIA) were selected and their GCF samples were collected from both deciduous cuspids and central incisors in the maxilla. Among them, neutrophil-delived proteins, myeloperoxidase and lactoferrin, were upregulated in deciduous GCF. Protein levels of myeloperoxidase and lactoferrin in individual GCF samples, again determined by ELISA, were significantly higher in deciduous GCF than in permanent GCF (P < 0.01), confirming the predominance of neutrophil-derived proteins in deciduous GCF. These findings suggest that neutrophil accumulation is beneficial for gingival tissue of deciduous teeth and shows difference of protective mechanism against bacterial infection between deciduous and permanent teeth.

Research Products

• [Presentation] 乳歯と永久歯の歯肉溝滲出液中に含まれるタンパク質の網羅的解析 (2015)
  • Author(s): 守屋佑美、小浜孝士、杉山智美、遠藤由美子、小出容子、野口江美子、石塚元規、井上美津子、板部洋之、山本松男
  • Organizer: 第58回秋季日本歯周病学会学術大会
  • Place of Presentation: 浜松
  • Year and Date: 2015-09-12
  • Int'l Joint Research
• [Presentation] Comparison between gingival crevicular fluids from deciduous and permanent teeth using iTRAQ quantitative proteomics (2015)
  • Author(s): Y. Moriya, T. Obama, N. Sasabe, T. Aiuchi, Y. Endo, T. Sugiyama, Y. Koide, M. Inoue, H. Itabe, M. Yamamoto
  • Organizer: Europerio 8
  • Place of Presentation: London, UK
  • Year and Date: 2015-06-03
  • Int'l Joint Research