| Project/Area Number |
26670897
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Social dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 しづ子 東北大学, 歯学研究科, 助教 (60225274)
庄司 憲明 東北大学, 大学病院, 講師 (70250800)
飯久保 正弘 東北大学, 歯学研究科, 講師 (80302157)
菅原 俊二 東北大学, 歯学研究科, 教授 (10241639)
市川 博之 東北大学, 歯学研究科, 教授 (20193435)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 味覚検査 / うま味受容体 / 遺伝子発現 / 客観的味覚診断 / 味覚障害 / 客観的味覚検査 / 味覚受容体遺伝子 / 遺伝子解析 / 客観的診断 / うま味 / 遺伝子診断 |
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| Outline of Final Research Achievements |
Expression of taste-related genes in the tongue was analysed to develop a technique for genetic diagnosis of umami taste disorders.Gustducin-positive cells were observed in the samples, indicating the presence of taste cells. Gene expression of b-actin, GNAT3, T1R1 and T1R3 was detected in all seven samples tested, while that of mGluR1 was detected in four samples. Sequence analysis by NCBI Blast showed that each polymerase chain reaction product had a 99% rate of identification of its target sequence. Stimulation of the tongue with monosodium glutamate significantly upregulated the gene expression levels of T1R1 and T1R3, indicating that this method can detect alterations in umami-related gene expression. Thus, evaluation of the expression of the umami receptor genes, T1R1 and T1R3, in the tongue may be clinically useful for objective genetic diagnosis of umami taste disorders.
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