Principle and application of skin blotting method
Project/Area Number |
26670914
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Fundamental nursing
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Research Institution | The University of Tokyo |
Principal Investigator |
MINEMATSU Takeo 東京大学, 医学(系)研究科(研究院), 講師 (00398752)
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Co-Investigator(Kenkyū-buntansha) |
SANADA Hiromi 東京大学, 大学院医学系研究科, 教授 (50143920)
MORI Taketoshi 東京大学, 大学院医学系研究科, 教授 (20272586)
NAKAGAMI Gojiro 東京大学, 大学院医学系研究科, 講師 (70547827)
NOGUCHI Hiroshi 東京大学, 大学院医学系研究科, 講師 (50431797)
TAMAI Nao 東京大学, 大学院医学系研究科, 助教 (80636788)
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Project Period (FY) |
2014-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | スキンアセスメント / 褥瘡 / スキンケア / 看護技術 / スキンブロッティング / 皮膚バリア機能 / 角質細胞間脂質 / タイトジャンクション |
Outline of Final Research Achievements |
(1)The principle of skin blotting: We could not fully reveal the principle of skin blotting within this period. Conventional techniques are not enough for the analysis of the effect of skin blotting on the inter-corneocyte lipid lamellae structure. Therefore, we developed new fixation technique of skin tissue, which can keep the structures of inter-corneocyte lipid, epidermis and dermis. Furthermore, the temporal structural change by skin blotting was suggested by immunofluorescence for claudin-1, a tight junction protein. (2)In the animal experiment, we identified the biomarkers for early detection of pressure ulcer development by skin blotting. The biomarkers are PAI1, VEGF-C, IL1a, and HSP90a, which reflected the ischemia, lymphatic dysfunction, ischemia-reperfusion injury, and deformation of tissue, respectively.
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Report
(3 results)
Research Products
(18 results)
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[Journal Article] Hypoosmotic shock-induced subclinical inflammation of skin in a rat model of disrupted skin barrier function2015
Author(s)
Kishi C, Minematsu T, Huang L, Mugita Y, Kitamura A, Nakagami G, Yamane T, Yoshida M, Noguchi H, Funakubo M, Mori T, Sanada H
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Journal Title
Biological Research For Nursing
Volume: 17(2)
Issue: 2
Pages: 135-141
DOI
Related Report
Peer Reviewed
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[Journal Article] Exploring the prevalence of skin tears and skin properties related to skin tears in elderly patients at a long-term medical facility in Japan.2014
Author(s)
Koyano Y, Nakagami G, Iizaka S, Minematsu T, Noguchi H, Tamai N, Mugita Y, Kitamura A, Tabata K, Abe M, Murayama R, Sugama J, Sanada H.
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Journal Title
International Wound Journal
Volume: -
Issue: 2
Pages: 189-197
DOI
Related Report
Peer Reviewed
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[Presentation] Skin blotting: A novel technique to assess physiological skin conditions.2014
Author(s)
Minematsu T, Mugita Y, Koyano Y, Kishi C, Horii M, Oe M, Sugama J, Nakagami G, Noguchi H, Mori T, Sanada H.
Organizer
Australian Wound Management Association (AMWA) 2014
Place of Presentation
Gold Coast (Australia)
Year and Date
2014-05-07 – 2014-05-10
Related Report
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[Book] 看護理工学2015
Author(s)
真田弘美,森武俊
Total Pages
234
Publisher
東京大学出版会
Related Report
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