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Principle and application of skin blotting method

Research Project

Project/Area Number 26670914
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Fundamental nursing
Research InstitutionThe University of Tokyo

Principal Investigator

MINEMATSU Takeo  東京大学, 医学(系)研究科(研究院), 講師 (00398752)

Co-Investigator(Kenkyū-buntansha) SANADA Hiromi  東京大学, 大学院医学系研究科, 教授 (50143920)
MORI Taketoshi  東京大学, 大学院医学系研究科, 教授 (20272586)
NAKAGAMI Gojiro  東京大学, 大学院医学系研究科, 講師 (70547827)
NOGUCHI Hiroshi  東京大学, 大学院医学系研究科, 講師 (50431797)
TAMAI Nao  東京大学, 大学院医学系研究科, 助教 (80636788)
Project Period (FY) 2014-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsスキンアセスメント / 褥瘡 / スキンケア / 看護技術 / スキンブロッティング / 皮膚バリア機能 / 角質細胞間脂質 / タイトジャンクション
Outline of Final Research Achievements

(1)The principle of skin blotting: We could not fully reveal the principle of skin blotting within this period. Conventional techniques are not enough for the analysis of the effect of skin blotting on the inter-corneocyte lipid lamellae structure. Therefore, we developed new fixation technique of skin tissue, which can keep the structures of inter-corneocyte lipid, epidermis and dermis. Furthermore, the temporal structural change by skin blotting was suggested by immunofluorescence for claudin-1, a tight junction protein.
(2)In the animal experiment, we identified the biomarkers for early detection of pressure ulcer development by skin blotting. The biomarkers are PAI1, VEGF-C, IL1a, and HSP90a, which reflected the ischemia, lymphatic dysfunction, ischemia-reperfusion injury, and deformation of tissue, respectively.

Report

(3 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • Research Products

    (18 results)

All 2016 2015 2014 Other

All Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) Book (1 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Increased level of tumor necrosis factor-alpha (TNF-α) on the skin of Japanese obese males: measured by quantitative skin blotting.2016

    • Author(s)
      Ogai K, Matsumoto M, Aoki M, Minematsu T, Kitamura K, Kobayashi M, Sanada H and Sugama J
    • Journal Title

      Int J Cosmet Sci.

      Volume: in press Issue: 5 Pages: 462-469

    • DOI

      10.1111/ics.12312

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of an improved method for quantitative analysis of skin blotting: Increasing reliability and applicability for skin assessment.2015

    • Author(s)
      Ogai K, Matsumoto M, Minematsu H, Kitamura K, Kobayashi M, Sugama J and Sanada H
    • Journal Title

      International Journal of Cosmetic Science

      Volume: - Issue: 4 Pages: 425-432

    • DOI

      10.1111/ics.12217

    • NAID

      120005704522

    • Related Report
      2015 Annual Research Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Compression-induced HIF-1 enhances thrombosis and PAI-1 expression in mouse skin.2015

    • Author(s)
      49)Kaneko M, Minematsu T, Yoshida M, Nishijima Y, Noguchi H, Ohta Y, Nakagami G, Mori T, Sanada H.
    • Journal Title

      Wound Repair and Regeneration

      Volume: 23 Issue: 5 Pages: 657-663

    • DOI

      10.1111/wrr.12312

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hypoosmotic shock-induced subclinical inflammation of skin in a rat model of disrupted skin barrier function2015

    • Author(s)
      Kishi C, Minematsu T, Huang L, Mugita Y, Kitamura A, Nakagami G, Yamane T, Yoshida M, Noguchi H, Funakubo M, Mori T, Sanada H
    • Journal Title

      Biological Research For Nursing

      Volume: 17(2) Issue: 2 Pages: 135-141

    • DOI

      10.1177/1099800414532827

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Skin blotting: a noninvasive technique for evaluating physiological skin status2014

    • Author(s)
      Minematsu T, Horii M, Oe M, Sugama J, Mugita Y, Huang L, Nakagami G, Sanada H
    • Journal Title

      Advances in skin & wound care

      Volume: 27(6) Issue: 6 Pages: 272-279

    • DOI

      10.1097/01.asw.0000448461.25542.36

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Exploring the prevalence of skin tears and skin properties related to skin tears in elderly patients at a long-term medical facility in Japan.2014

    • Author(s)
      Koyano Y, Nakagami G, Iizaka S, Minematsu T, Noguchi H, Tamai N, Mugita Y, Kitamura A, Tabata K, Abe M, Murayama R, Sugama J, Sanada H.
    • Journal Title

      International Wound Journal

      Volume: - Issue: 2 Pages: 189-197

    • DOI

      10.1111/iwj.12251

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] 角質細胞間脂質の透過型電子顕微鏡観察を目的とした皮膚組織の新たな固定法:乾湿固定法.2015

    • Author(s)
      峰松健夫,髙木孝士,真田弘美.
    • Organizer
      第61回日本病理学会秋期特別総会
    • Place of Presentation
      東京大学安田講堂(東京都文京区)
    • Year and Date
      2015-11-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] ニトロセルロースメンブレンの極性による表皮タイトジャンクションの変化と回復:スキンブロッティングの原理.2015

    • Author(s)
      峰松健夫,仲上豪二朗,真田弘美.
    • Organizer
      第3回看護理工学会学術集会
    • Place of Presentation
      立命館大学朱雀キャンパス(京都府京都市)
    • Year and Date
      2015-10-10
    • Related Report
      2015 Annual Research Report
  • [Presentation] ニトロセルロースメンブレンの貼付による皮膚バリア機能の変化-スキンブロッティング法の原理解明に向けて-2014

    • Author(s)
      峰松健夫,仲上豪二朗,池田真一,真田弘美.
    • Organizer
      第44回日本創傷治癒学会
    • Place of Presentation
      ホテルメトロポリタン仙台(宮城県・仙台市)
    • Year and Date
      2014-12-02 – 2014-12-03
    • Related Report
      2014 Research-status Report
  • [Presentation] スキンブロッティング法によるマウス背部皮膚の毛包タンパク質の検出.2014

    • Author(s)
      峰松健夫,田崎なつみ,池田真一,真田弘美.
    • Organizer
      第19回日本臨床毛髪学会学術集会
    • Place of Presentation
      岡山コンベンションセンター(岡山県・岡山市)
    • Year and Date
      2014-11-23 – 2014-11-24
    • Related Report
      2014 Research-status Report
  • [Presentation] 褥瘡の発生・治癒メカニズムに基づく非侵襲的アセスメント2014

    • Author(s)
      峰松健夫
    • Organizer
      第16回日本褥瘡学会学術集会
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2014-08-29 – 2014-08-30
    • Related Report
      2014 Research-status Report
  • [Presentation] マウス圧迫皮膚における血栓形成と低酸素誘導因子活性化の相互増幅作用-新たな褥瘡発生メカニズムの提唱2014

    • Author(s)
      金子真紀,峰松健夫,大田泰徳,西島良美,Naresh K Rajendran,仲上豪二朗,真田弘美
    • Organizer
      第16回日本褥瘡学会学術集会
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2014-08-29 – 2014-08-30
    • Related Report
      2014 Research-status Report
  • [Presentation] スキンブロッティング法を用いた体圧分散マットレスの褥瘡予防効果の評価2014

    • Author(s)
      堀紀子,玉井奈緒,峰松健夫,仲上豪二朗,野口博史,森武俊,真田弘美
    • Organizer
      第16回日本褥瘡学会学術集会
    • Place of Presentation
      名古屋国際会議場(愛知県・名古屋市)
    • Year and Date
      2014-08-29 – 2014-08-30
    • Related Report
      2014 Research-status Report
  • [Presentation] Skin blotting: A novel technique to assess physiological skin conditions.2014

    • Author(s)
      Minematsu T, Mugita Y, Koyano Y, Kishi C, Horii M, Oe M, Sugama J, Nakagami G, Noguchi H, Mori T, Sanada H.
    • Organizer
      Australian Wound Management Association (AMWA) 2014
    • Place of Presentation
      Gold Coast (Australia)
    • Year and Date
      2014-05-07 – 2014-05-10
    • Related Report
      2014 Research-status Report
  • [Book] 看護理工学2015

    • Author(s)
      真田弘美,森武俊
    • Total Pages
      234
    • Publisher
      東京大学出版会
    • Related Report
      2015 Annual Research Report
  • [Remarks] 東京大学大学院医学系研究科老年看護学/創傷看護学分野

    • URL

      http://www.rounenkango.m.u-tokyo.ac.jp/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 東京大学医学系研究科老年看護学/創傷看護学分野ホームページ

    • URL

      http://www.rounenkango.m.u-tokyo.ac.jp/

    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] 4)A marker for predicting pressure ulcer development and a use thereof.2016

    • Inventor(s)
      真田弘美、仲上豪二朗、峰松健夫
    • Industrial Property Rights Holder
      真田弘美、仲上豪二朗、峰松健夫
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-01-14
    • Related Report
      2015 Annual Research Report
    • Overseas

URL: 

Published: 2014-04-04   Modified: 2017-05-10  

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