Influence of aging and exercise on engraftment after stem cell transplantation into myocardial infarction model mice

• Project/Area Number: 26671017
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gerontological nursing
• Research Institution: Yokohama City University
• Principal Investigator: CHIBA Yumi 横浜市立大学, 医学研究科, 教授 (10313256)
• Co-Investigator(Kenkyū-buntansha): 豊田 雅士 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究副部長 (50392486) ; 石上 友章 横浜市立大学, 医学部, 准教授 (50264651) ; 峯岸 慎太郎 横浜市立大学, 附属病院, 助教 (80458398)
• Co-Investigator(Renkei-kenkyūsha): SASAKI Norihiko 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (80639063) ; ITAKURA Yoko 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (30582746)
• Research Collaborator: NAKASHIMA Rie 横浜市立大学, 大学院・医学研究科, 大学院生
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 心筋梗塞 / 幹細胞 / 再生 / モデルマウス / 細胞培養 / 切片

Outline of Final Research Achievements

Heart diseases in the elderly are at the top of the cause of death, and treatment options are limited by severity. One means to solve this problem can be said to establish a treatment method by myocardial regeneration by stem cell transplantation. In this study, the aim is to lead appropriateness of the safety and effectiveness of stem cell transplantation in human heart disease pathology by making and evaluating disease model animals to solve this task. Until now, myocardial infarction occurred in young and old mice, and almost completed the evaluation system of heart functions based on ultrasonic evaluation and so on. On the other hand, with regard to the establishment and culture of stem cells as a source of stem cell transplantation to the site of myocardial infarction, both isolation and culture started smoothly, and they are progressing without problems. In the future we will continue to transplant to a disease model.

Research Products

• [Journal Article] A standardized method for lectin microarray-based tissue glycome mapping. (2017)
  • Author(s): Zou X, Yoshida M, Nagai-Okatani C, Iwaki J, Matsuda A, Tan B, Hagiwara K, Sato T, Itakura Y, Noro E, Kaji H, Toyoda M, Zhang Y, Narimatsu H, Kuno A
  • Journal Title: Sci Rep Volume: 7 Issue: 1 Pages: 1-12
  • DOI: 10.1038/srep43560
  • Peer Reviewed / Open Access
• [Journal Article] α2-6sialylation is a marker of the differentiation potential of human mesenchymal stem cells (2016)
  • Author(s): Tateno H, Saito S, Hiemori K, Kiyoi K, Hasehira K, Toyoda M, Onuma Y, Ito Y, Akutsu H, Hirabayashi J
  • Journal Title: Glycobiology Volume: 26 Pages: 1328-1337
  • DOI: 10.1093/glycob/cww039
  • Peer Reviewed
• [Journal Article] Embryoid Body-Explant Outgrowth Cultivation from Induced Pluripotent Stem Cells in an Automated Closed Platform. (2016)
  • Author(s): Tone H, Yoshioka S, Akiyama H, Nishimura A, Ichimura M, Nakatani M, Kiyono T, Toyoda M, Watanabe M, Umezawa A.
  • Journal Title: Biomed Res Int. Volume: 2016 Pages: 1-7
  • DOI: 10.1155/2016/7098987
  • Peer Reviewed / Open Access
• [Journal Article] N- and O-glycan cell surface protein modifications associated with cellular senescence and human aging. (2016)
  • Author(s): Itakura Y, Sasaki N, Kami D, Gojo S, Umezawa A, Toyoda M
  • Journal Title: Cell Biosci. Volume: 6(14) Issue: 1 Pages: 14-14
  • DOI: 10.1186/s13578-016-0079-5
  • Peer Reviewed / Open Access
• [Journal Article] Ganglioside GM1 contributes to the state of insulin resistance in senescent human arterial endothelial cells. (2015)
  • Author(s): Sasaki N, Itakura Y, Toyoda M.
  • Journal Title: J. Biol. Chem. Volume: 290 Issue: 42 Pages: 25475-25486
  • DOI: 10.1074/jbc.m115.684274
  • Peer Reviewed / Open Access
• [Journal Article] Novel detergent for whole organ tissue engineering. (2015)
  • Author(s): Kawasaki T, Kirita Y, Kami D, Kitani T, Ozaki C, Itakura Y, Toyoda M, Gojo S.
  • Journal Title: J. Biomed. Mater. Res. A. Volume: 103(10) Issue: 10 Pages: 3364-3373
  • DOI: 10.1002/jbm.a.35474
  • Peer Reviewed / Open Access
• [Journal Article] Pleiotropic functions of magnetic nanoparticles for ex vivo gene transfer. (2014)
  • Author(s): Kami D, Kitani T, Kishida T, Mazda O, Toyoda M, Tomitaka A, Ota S, Ishii R, Takemura Y, Watanabe M, Umezawa A, Gojo S.
  • Journal Title: Nanomedicine Volume: 10 Issue: 6 Pages: 1165-1174
  • DOI: 10.1016/j.nano.2014.03.018
  • Peer Reviewed / Open Access
• [Journal Article] Stem Cells Bond Our Organs/Tissues and Engineering Products (2014)
  • Author(s): Toyoda M, Umezawa A.
  • Journal Title: Circulation Journal Volume: 78 Issue: 7 Pages: 1582-1583
  • DOI: 10.1253/circj.CJ-14-0558
  • NAID: 130004926975
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access
• [Presentation] Nedd4-2 C2ノックアウトマウスを用いた心房細動の誘発に対する影響の検討 (2016)
  • Author(s): 川村飛翔, 峯岸慎太郎, 木野旅人, 中島理恵, 陳琳, 土肥宏志, 杉山美智子, Rajesh Prajapati, 藤田孝之, 石川義弘, 千葉由美, 石上友章, 田村功一
  • Organizer: 第20回日本心血管内分泌代謝学会学術集会
  • Place of Presentation: 東京コンベンションホール（東京都中央区）
  • Year and Date: 2016-12-17
• [Presentation] Comparison between cerebrovascular and heart disease regarding dysphagia related clinical conditions among hospitalized patients in general hospitals. (2016)
  • Author(s): Yumi Chiba, Ritsuko Yamada, Kumiko Ichimura
  • Organizer: 第36回日本看護科学学会学術集会English Session（Oral）
  • Place of Presentation: 東京国際フォーラム（東京都千代田区）
  • Year and Date: 2016-12-10
• [Presentation] 心疾患患者への幹細胞移植による効果指標の検討-国内文献レビューから看護の取り組みを考える- (2016)
  • Author(s): 千葉由美
  • Organizer: 第12回日本移植・再生医療看護学会
  • Place of Presentation: ウインク愛知（愛知県名古屋市）
  • Year and Date: 2016-11-12