Elucidation of hybrid N2O production mechanisms by ammonia-oxidizing bacteria

• Project/Area Number: 26701003
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Environmental dynamic analysis
• Research Institution: Hiroshima University
• Principal Investigator: Katsuyama Chie 広島大学, 総合科学研究科, 助教 (10580061)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000); Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000); Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: 窒素安定同位体トレーサー / 亜酸化窒素 / アンモニア酸化細菌 / Nitrosomonas / 硝化 / 脱窒 / 窒素循環 / ガスクロマトグラフ質量分析計 / 好気性アンモニア酸化細菌 / 酸素安定同位体トレーサー / 環境分析 / 微生物 / 国際情報交換（USA、カナダ）

Outline of Final Research Achievements

Mechanisms of hybrid nitrous oxide (N2O) production by aerobic ammonia-oxidizing bacteria, a combination of one nitrogen from hydroxylamine and the other from nitrite, were analyzed by using 15N tracer. In the presence of nitrite, hybrid N2O was aerobically produced with hydroxylamine from ammonia, but not with ammonia directly. Processing ammonia and N-oxides and ratio of hybrid N2O in total N2O production were different between Nitrosomonas strains.

Research Products

• [Journal Article] Hybrid Nitrous Oxide Production from a Partial Nitrifying Bioreactor: Hydroxylamine Interactions with Nitrite (2017)
  • Author(s): A. Terada, S. Sugawara, K. Hojo, Y. Takeuchi, S. Riya, W. F. HarperJr., T. Yamamoto, M. Kuroiwa, Isobe, C. Katsuyama, Y. Suwa, K. Koba and M. Hosomi
  • Journal Title: Environmental Science and Technology Volume: ５１ Issue: 5 Pages: 2748-2756
  • DOI: 10.1021/acs.est.6b05521
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 好気性アンモニア酸化細菌による好気条件でのhybrid N2O生成経路の検討 (2017)
  • Author(s): 菅野麻子, 岩本茉莉, 久我ゆかり, 諏訪裕一, 勝山千恵
  • Organizer: 第11回日本ゲノム微生物学会年会
  • Place of Presentation: 慶應義塾大学湘南藤沢キャンパス, 神奈川
  • Year and Date: 2017-03-02
• [Presentation] Effects of ammonium and hydroxylamine on N2O-producing pathways in Nitrosomonas europaea ATCC 19718 and Nitrosomonas sp. AL212 revealed by a 15N tracer technique (2017)
  • Author(s): Chie Katsuyama, Mako Kanno, Akihiko Terada, Keisuke Koba, Yukari Kuga, Lisa Y. Stein, Martin G. Klotz, Yuichi Suwa
  • Organizer: Fifth International Conference on Nitrification and Related Processes (ICoN5)
  • Int'l Joint Research