Elucidating N2O production mechanisms by ammonia-oxidizing bacteria based on nitrogen atoms and electron flows

• Project/Area Number: 26701009
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Environmental engineering and reduction of environmental burden
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Terada Akihiko 東京農工大学, 工学(系)研究科(研究院), 准教授 (30434327)
• Research Collaborator: KOBA Keisuke 京都大学, 生態学研究センター, 教授 (90311745) ; SUWA Yuichi 中央大学, 理工学部, 教授 (90154632) ; HORI Tomoyuki 国立研究開発法人産業技術総合研究所, 環境管理研究部門, 主任研究員 (20509533)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000); Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2015: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2014: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: 亜酸化窒素 / アンモニア酸化細菌 / 部分硝化 / 15Nトレーサー / 省エネ型排水処理 / 窒素除去 / ハイブリッド反応 / ヒドロキシルアミン / 亜酸化窒素（N2O） / 生成経路 / 硝化菌脱窒 / ヒドロキシルアミン酸化 / 同位体比アナライザー / 15Nトレーサー法 / 同位体比解析 / オンラインモニタリング / バイオリアクター / 亜硝酸イオン / 安定同位体 / N2O生成経路 / 亜硝酸

Outline of Final Research Achievements

Mitigation of nitrous oxide (N2O), known as a highly-potent greenhouse gas and ozone-depleting substance, is demanded. This study elucidated production pathways of N2O emitted during partial nitrification (PN) as a promising technology to removal nitrogen from wastewater in a cost-effective way. To unveil intricate N2O production pathways during PN, mainly mediated by ammonia-oxidizing bacteria, a 15N tracer method was employed, capable of measuring N2O isotopologues. The application of the method to batch tests for PN demonstrated that N-nitrosation hybrid reaction, coupling hydroxylamine with nitrite, mainly occurred in a PN bioreactor. This hybrid reaction was likely to occur also via an abiotic reaction, which has been incorporated into a new N2O production model. Furthermore, the experiment on dissolved oxygen (DO) effect on N2O production and pathways underpinned that proportions of N2O production pathways vary depending on DO concentrations.

Research Products

• [Int'l Joint Research] Air Force Institute of Technology(米国)
• [Int'l Joint Research] Technical University of Denmark(デンマーク)
• [Int'l Joint Research] Technical University of Denmark(Denmark)
• [Int'l Joint Research] Air Force Institute of Technology(米国)
• [Journal Article] Free nitrous acid and pH determine the predominant ammonia-oxidizing bacteria and amount of N2O in a partial nitrifying reactor (2017)
  • Author(s): C.T. Kinh, J. Ahn, T. Suenaga, N. Sittivorakulpong, P. Noophan, T. Hori, S. Riya, M. Hosomi, A. Terada
  • Journal Title: Applied Microbiology and Biotechnology Volume: 101 Issue: 4 Pages: 1673-1683
  • DOI: 10.1007/s00253-016-7961-2
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Hybrid Nitrous Oxide Production from a Partial Nitrifying Bioreactor: Hydroxylamine Interactions with Nitrite (2017)
  • Author(s): A. Terada, S. Sugawara, K. Hojo, Y. Takeuchi, S. Riya, W. F. HarperJr., T. Yamamoto, M. Kuroiwa, Isobe, C. Katsuyama, Y. Suwa, K. Koba and M. Hosomi
  • Journal Title: Environmental Science and Technology Volume: ５１ Issue: 5 Pages: 2748-2756
  • DOI: 10.1021/acs.est.6b05521
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effect of biological and mass transfer parameter uncertainty on nitrous oxide emission estimates from water resource recovery facilities (2016)
  • Author(s): Song, K., Harper Jr., W.F. Takeuchi, Y., Hosomi, M., Terada, A.
  • Journal Title: Water Environment Research Volume: 印刷中 Issue: 7 Pages: 580-585
  • DOI: 10.2175/106143015x14338845155066
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Novel abiotic reactions increase nitrous oxide production during partial nitrification: Modeling and experiments (2015)
  • Author(s): Harper Jr., W.F., Takeuchi, Y., Riya, S., Hosomi, M., Terada, A.
  • Journal Title: Chemical Engineering Journal Volume: 281 Pages: 1017-1023
  • DOI: 10.1016/j.cej.2015.06.109
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Effect of carbon sources on nitrous oxide emission in a Modified Ludzak Ettinger process (2015)
  • Author(s): Kang SONG，Shohei Riya, Masaaki Hosomi, Akihiko Terada,
  • Journal Title: Water Science and Technology Volume: 72 Issue: 4 Pages: 572-578
  • DOI: 10.2166/wst.2015.250
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 硝化プロセスにおける亜酸化窒素の放出特性と生成機構 (2015)
  • Author(s): 寺田昭彦
  • Journal Title: 水環境学会誌 Volume: 38(9) Pages: 334-339
• [Journal Article] Effect of carbon sources on nitrous oxide emission in a Modified Ludzak Ettinger process (2015)
  • Author(s): Kang Song, Shohei Riya, Masaaki Hosomi, Akihiko Terada
  • Journal Title: Water Science and Technology Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effect of biological and mass transfer parameter uncertainty on N2O emission estimates from WWTPs (2015)
  • Author(s): Kang Song, Willie F. Harper, Yuki Takeuchi, Masaaki Hosomi, Akihiko Terada
  • Journal Title: Water Environment Research Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Nitrous oxide emission from cost-effective nitrogen removal systems: Mechanisms and mitigation strategies (2017)
  • Author(s): Akihiko Terada
  • Organizer: 2017 International Conference on Environmental Pollution Control
  • Place of Presentation: バンクーバー（カナダ）
  • Year and Date: 2017-10-08
  • Int'l Joint Research / Invited
• [Presentation] Identifying hotspots for production and consumption of NO and N2O in a membrane-aerated biofilm for nitrogen removal (2017)
  • Author(s): Co Thi Kinh, Toshikazu Suenaga, Tomoyuki Hori, Shohei Riya, Masaaki Hosomi, Akihiko Terada
  • Organizer: 10th International Conference on Biofilm Reactors
  • Place of Presentation: ダブリン（アイルランド）
  • Year and Date: 2017-05-09
  • Int'l Joint Research
• [Presentation] 15Nトレーサー法と同位体比アナライザーの併用による部分硝化リアクターの亜酸化窒素生成機構解明 (2017)
  • Author(s): 中村洋貴、丸尾光希、細見正明、寺田昭彦
  • Organizer: 第51回日本水環境学会年会
  • Place of Presentation: 熊本大学（熊本）
  • Year and Date: 2017-03-15
• [Presentation] Spatial distribution of microbial community and N2O depth profiles in counter- and co- diffusion biofilms functioning simultaneous nitrification and denitrification (2016)
  • Author(s): Co Thi Kinh, Toshikazu Suenaga, Tomoyuki Hori, Shohei Riya, Barth Smets, Masaaki Hosomi, Akihiko Terada
  • Organizer: MEWE-Biofilm
  • Place of Presentation: コペンハーゲン（デンマーク）
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] A biofilm employing counter-current substrate diffusion geometry facilitates N2O mitigation: Proof of concept (2016)
  • Author(s): Co Thi Kinh, Toshikazu Suenaga, Yuki Takeuchi, Tomoyuki Hori, Shohei Riya, Masaaki Hosomi, Akihiko Terada
  • Organizer: Water Environment Technology Conference 2016
  • Place of Presentation: 中央大学（東京）
  • Year and Date: 2016-08-27
  • Int'l Joint Research
• [Presentation] Depth profiles of nitrous oxide and functional genes in counter- and co- diffusion biofilms performing nitrification and denitrification (2016)
  • Author(s): Co Thi Kinh, Toshikazu Suenaga, Tomoyuki Hori, Shohei Riya, Barth Smets, Masaaki Hosomi, Akihiko Terada
  • Organizer: WEF/IWA Nutrient Removal and Recovery 2016
  • Place of Presentation: デンバー（アメリカ）
  • Year and Date: 2016-06-10
  • Int'l Joint Research
• [Presentation] 部分硝化リアクターから放出される亜酸化窒素の生成メカニズム： 非生物学的反応経路の関与 (2016)
  • Author(s): 竹内裕貴、丸尾光希、利谷翔平、細見正明、寺田昭彦
  • Organizer: 第50回 日本水環境学会年会
  • Place of Presentation: アスティ徳島（徳島県徳島市）
  • Year and Date: 2016-03-16
• [Presentation] Nitrous oxide production by multiple pathways in a partial nitrifying bioreactor: Significance of abiotic reaction (2015)
  • Author(s): Akihiko Terada, Willie Harper, Yuki Takeuchi, Kang Song, Shohei Riya, Masaaki Hosomi
  • Organizer: 6th IWA-ASPIRE Conference & Exhibition
  • Place of Presentation: Beijing, China
  • Year and Date: 2015-09-20 – 2015-09-24
• [Presentation] Nitrous oxide production by multiple pathways in a partial nitrifying bioreactor: Significance of abiotic reaction (2015)
  • Author(s): Terada, A., Harper Jr., W.F., Takeuchi, Y., Riya, S., Hosomi, M.
  • Organizer: The 6th IWA-ASPIRE Conference & Exhibition
  • Place of Presentation: Beijing, China
  • Year and Date: 2015-09-20
  • Int'l Joint Research
• [Presentation] Nitrous oxide production potential and pathways of ammonia-oxidizing bacteria enriched in a bioreactor towards partial nitrification (2015)
  • Author(s): Terada, A., Kinh, C.T., Yamamoto, T., Hojo, K., Riya, S., Hosomi, M.
  • Organizer: PRES’15
  • Place of Presentation: Kuching, Malaysia
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] Identification of a predominant nitrous oxide production pathway from a partial nitrifying bioreactor: A 15N tracer study (2015)
  • Author(s): Terada, A., Takeuchi, Y., Hojo, K., Riya, S., Koba, K., Hosomi, M.
  • Organizer: International Conference on Nitrification 4
  • Place of Presentation: Edmonton, Canada
  • Year and Date: 2015-06-28
  • Int'l Joint Research
• [Presentation] 15Nトレーサー法による回分式・連続式部分硝化リアクターからの亜硝酸窒素生成機構の解明 (2014)
  • Author(s): 竹内裕貴、北條圭佑、利谷翔平、Willie Harper、細見正明、寺田昭彦
  • Organizer: 環境微生物系学会合同大会2014
  • Place of Presentation: アクトシティ浜松（静岡県浜松市）
  • Year and Date: 2014-10-22 – 2014-10-24
• [Presentation] Nitrous oxide production pathways of nitrifying activated sludge in a sequencing batch reactor (2014)
  • Author(s): Akihiko Terada, Keisuke Hojo, Tomoko Yamamoto, Shohei Riya, Keisuke Koba, Masaaki Hosomi
  • Organizer: IWA World Water Congress
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 2014-09-21 – 2014-09-25