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Alanyzing the substrate multispecificity of human peptide transporter

Research Project

Project/Area Number 26702002
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Eating habits
Research InstitutionUniversity of Shizuoka

Principal Investigator

Ito Keisuke  静岡県立大学, 食品栄養科学部, 准教授 (40580460)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Keywordsペプチド輸送体 / 必須アミノ酸 / 食品 / 生体機能利用 / ペプチド / ペプチド栄養 / 分岐鎖アミノ酸 / 芳香族アミノ酸 / 機能性ペプチド / トランスポーター / 機能性食品 / ハイスループットスクリーニング / Saccharomyces cerevisiae / 食品成分の吸収 / ジペプチド
Outline of Final Research Achievements

Proton-coupled oligopeptide transporter (POT) family proteins are characterized by their substrate multispecificity that one transporter can recognize as many as 8,400 types of di/tripeptides as substrates. This unique characteristic enables certain types of drugs to be absorbed by the human intestine and kidney. In this study, we elucidated the substrate multispecificity of human PEPT2 by the comprehensive analysis using a dipeptide library. Dipeptides that comprised aromatic amino acids and branched-chain amino acids displayed high-affinity for hPEPT2. The biosynthesis of aromatic amino acids and branched-chain amino acids requires the expression of multiple enzymes and involves energy-consuming reactions. Therefore, the substrate multispecificity of hPEPT2 is suitable to import the high-value amino acids.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (19 results)

All 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 2 results) Presentation (13 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Synergistic inhibition of human dipeptidyl peptidase IV by combinations of peptides.2015

    • Author(s)
      M. Oho, K. Ito*†, V.T.T. Lan, M. Kusubata, C. Tometsuka, Y. Koyama, T. Motoyama, S. Ito, Y. Kawarasaki.
    • Journal Title

      Peptides

      Volume: 未定 Pages: 115-117

    • DOI

      10.1016/j.peptides.2015.04.015

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Analyzing a dipeptide library to identify human dipeptidyl peptidase IV inhibitor.2015

    • Author(s)
      V.T.T. Lan*, K. Ito*†, M. Ohno, T. Motoyama, S. Ito, Y. Kawarasaki.
    • Journal Title

      Food Chem.

      Volume: 15 Pages: 66-73

    • DOI

      10.1016/j.foodchem.2014.11.131

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Trp-Arg-Xaa tripeptides act as uncompetitive-type inhibitors of human dipeptidyl peptidase IV2014

    • Author(s)
      Vu Thi Tuyet Lan, Keisuke Ito, Sohei Ito, Yasuaki Kawarasaki
    • Journal Title

      Peptides

      Volume: 54 Pages: 166-170

    • DOI

      10.1016/j.peptides.2014.01.027

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Presentation] 苦味マスキング剤探索・解析ツールとしてのペプチドアレイの応用2016

    • Author(s)
      伊藤圭祐、小池麻友、黒田侑希、石井剛志、中村順行、渡辺達夫、河原崎泰昌
    • Organizer
      第70回日本栄養・食糧学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2016-05-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] 食素材蛋白質の網羅的ペプチドアレイを用いた苦味マスキング剤の新規探索法 ~茶殻RubisCo由来EGCG結合ペプチドの解析例~2016

    • Author(s)
      小池麻友、黒田侑希、伊藤圭祐、石井剛志、中村順行、渡辺達夫、河原崎泰昌
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Substrate multispecificity of a proton-coupled oligopeptide transporter, Saccharomyces cerevisiae Ptr2p2015

    • Author(s)
      K. Ito, S. Kawai, T. Motoyama, Y. Yoshikawa, R. Kato, Y. Kawarasaki
    • Organizer
      The International Chemical Congress of Pacific Basin Societies 2015
    • Place of Presentation
      Honolulu
    • Year and Date
      2015-12-16
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 緑茶の苦渋味マスキング剤となる茶殻由来ペプチド2015

    • Author(s)
      小池麻友、黒田侑希、伊藤圭祐、石井剛志、中村順行、渡辺達夫、河原崎泰昌
    • Organizer
      平成27年度日本食品科学工学会中部支部大会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-12-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] ペプチドの生体吸収を担う腸管輸送体cePEPT1の基質多選択性2015

    • Author(s)
      伊藤圭祐、Vu Thi Tuyet Lan、河合駿、本山貴康、加藤竜司、河原崎泰昌
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-10-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] ヒトジペプチジルペプチダーゼIVを阻害する“緑茶ペプチド”の開発2015

    • Author(s)
      伊藤圭祐、細谷孝博、三好規之、石井剛志、中村順行、河原崎泰昌
    • Organizer
      第62回日本食品科学工学会大会
    • Place of Presentation
      京都
    • Year and Date
      2015-08-27 – 2015-08-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] ペプチドトランスポータPtr2pにおける基質選択性予測解析2015

    • Author(s)
      河合駿、伊藤圭祐、疋田礼、Vu Thi Tuyet Lan、本山貴康、北川さゆり、吉川悠子、加藤竜司、河原崎泰昌
    • Organizer
      2015年度生物工学若手の会夏のセミナー
    • Place of Presentation
      名古屋
    • Year and Date
      2015-07-11
    • Related Report
      2015 Annual Research Report
  • [Presentation] ジペプチドによるインクレチン加水分解酵素阻害データの網羅的取得とその応用2015

    • Author(s)
      伊藤圭祐、大野真澄、Vu Thi Tuyet Lan、本山貴康、楠畑雅、遠目塚千紗、小山洋一、伊藤創平、河原崎泰昌
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      岡山
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 他のインクレチン加水分解酵素阻害ペプチドの効果を増強する新タイプ機能性ペプチドの発見2014

    • Author(s)
      大野真澄、Vu Thi Tuyet Lan、伊藤圭祐、本山貴康、楠畑雅、遠目塚千紗、小山洋一、伊藤創平、河原崎泰昌
    • Organizer
      平成26年度日本食品科学工学会中部支部大会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-12-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトジペプチジルペプチダーゼIVを新たな様式で阻害するオリゴペプチド群の発見2014

    • Author(s)
      伊藤圭祐、大野真澄、Vu Thi Tuyet Lan、楠畑雅、遠目塚千紗、小山洋一、伊藤創平、河原崎泰昌
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトジペプチジルペプチダーゼIV阻害ジペプチドの網羅的解析2014

    • Author(s)
      Vu Thi Tuyet Lan、伊藤圭祐、大野真澄、本山貴康、河原崎泰昌
    • Organizer
      第66回日本生物工学会大会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトジペプチジルペプチダーゼIV阻害ジペプチドランキング2014

    • Author(s)
      伊藤圭祐、Vu Thi Tuyet Lan、大野真澄、本山貴康、楠畑雅、遠目塚千紗、小山洋一、伊藤創平、河原崎泰昌
    • Organizer
      第61回日本食品科学工学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2014-08-28 – 2014-08-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] プロトン共役型オリゴペプチドトランスポーターの基質多選択性2014

    • Author(s)
      伊藤圭祐
    • Organizer
      第9回トランスポーター研究会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2014-06-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Book] 日本食品・機械研究会誌2014

    • Author(s)
      伊藤圭祐、疋田礼、河合駿、Vu Thi Tuyet Lan、本山貴康、北川さゆり、吉川悠子、加藤竜司、河原崎泰昌
    • Total Pages
      8
    • Publisher
      日本食品機械研究会
    • Related Report
      2014 Annual Research Report
  • [Book] バイオサイエンスとインダストリー2014

    • Author(s)
      伊藤圭祐、河原崎泰昌
    • Total Pages
      2
    • Publisher
      バイオインダストリー協会
    • Related Report
      2014 Annual Research Report
  • [Remarks] 食品化学国際誌に掲載:ジペプチドによる抗2型糖尿病標的酵素の阻害

    • URL

      http://dfns.u-shizuoka-ken.ac.jp/wp/archives/779/

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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