| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
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| Outline of Final Research Achievements |
Proton-coupled oligopeptide transporter (POT) family proteins are characterized by their substrate multispecificity that one transporter can recognize as many as 8,400 types of di/tripeptides as substrates. This unique characteristic enables certain types of drugs to be absorbed by the human intestine and kidney. In this study, we elucidated the substrate multispecificity of human PEPT2 by the comprehensive analysis using a dipeptide library. Dipeptides that comprised aromatic amino acids and branched-chain amino acids displayed high-affinity for hPEPT2. The biosynthesis of aromatic amino acids and branched-chain amino acids requires the expression of multiple enzymes and involves energy-consuming reactions. Therefore, the substrate multispecificity of hPEPT2 is suitable to import the high-value amino acids.
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