Development of ion-sequensing method using boronic acid
| Project/Area Number |
26702013
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
Matsumoto Akira 東京医科歯科大学, 生体材料工学研究所, 准教授 (70436541)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
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| Keywords | ボロン酸 / ピロリン酸 / 核酸増幅 / シーケンサー / 生体計測 / DNA |
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| Outline of Final Research Achievements |
We found that a heterocyclic class of boronic acids, based on the pyridine structures, can interact with pyrophosphate and some of its derivatives in a specific, reversible and pH-dependent manner. Based on this finding, we prepared a series of boronate-modified electrodes and confirmed their sensitivity to pyrophosphate with the detection limit of 30 uM. Moreover, we designed a polymeric material that is able to undergo a sharp inversion in the state of hydration in synchronization with recognition of pyrophosphate. In combination with a common thermal cycle protocol in nucleotide amplification reactions, our finding may help dramatically simplify those ongoing technologies.
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Report
(4 results)
Research Products
(30 results)
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[Presentation] Preparation of environment-sensitive tetra-gel by using stimuli-sensitive macromer2016
Author(s)
Sakamaki T, Matsumoto A, Kondo S, Sakai T, Sato S, Kikuchi A, Tei Y, Goda T, Miyahara Y
Organizer
3rd International Conference on Biomaterials Science in Tokyo
Place of Presentation
The University of Tokyo, Bunkyo-ku, Tokyo
Year and Date
2016-11-28
Related Report
Int'l Joint Research
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[Presentation] Electronics-free and synthetic polymeric materials based approach toward artificial pancreas2016
Author(s)
Matsumoto A, Suganami T, Matsumoto H, Tanaka M, Ochi K, Moro-oka Y, Ogawa Y, Ishii T, Kataoka K, Miyahara Y
Organizer
3rd International Conference on Biomaterials Science in Tokyo
Place of Presentation
The University of Tokyo, Bunkyo-ku, Tokyo
Year and Date
2016-11-28
Related Report
Int'l Joint Research
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[Presentation] Preparation of environment-sensitive tetra-gel by using stimuli-sensitive macromer2016
Author(s)
Sakamaki T, Matsumoto A, Kondo S, Sakai T, Sato S, Kikuchi A, Tei Y, Goda T, Miyahara Y
Organizer
International Symposium on Biomedical Engineering
Place of Presentation
Nihon University, Nagatsuda, Chiba
Year and Date
2016-11-10
Related Report
Int'l Joint Research
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[Presentation] Demonstration of environment-sensitive tetra-gel by using stimuli-sensitive macromer2016
Author(s)
Sakamaki T, Matsumoto A, Kondo S, Sakai T, Sato S, Kikuchi A, Tei Y, Goda T, Miyahara Y
Organizer
The International Symposium on Visualization in Joining & Welding Science through Advanced Measurements and Simulation in conjunction with Symposium on the Research Activities of Joint Usage
Place of Presentation
Hotel Hankyu Expo Park, Suita, Osaka
Year and Date
2016-10-17
Related Report
Int'l Joint Research
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