| Budget Amount *help |
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
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| Outline of Final Research Achievements |
We found that a heterocyclic class of boronic acids, based on the pyridine structures, can interact with pyrophosphate and some of its derivatives in a specific, reversible and pH-dependent manner. Based on this finding, we prepared a series of boronate-modified electrodes and confirmed their sensitivity to pyrophosphate with the detection limit of 30 uM. Moreover, we designed a polymeric material that is able to undergo a sharp inversion in the state of hydration in synchronization with recognition of pyrophosphate. In combination with a common thermal cycle protocol in nucleotide amplification reactions, our finding may help dramatically simplify those ongoing technologies.
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