Molecular mechanisms of exercise-induced muscle hypertrophy

• Project/Area Number: 26702028
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Sports science
• Research Institution: Nagoya Institute of Technology (2015-2017); The University of Tokyo (2014)
• Principal Investigator: Ogasawara Riki 名古屋工業大学, 工学(系)研究科(研究院), 准教授 (10634602)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥22,880,000 (Direct Cost: ¥17,600,000、Indirect Cost: ¥5,280,000); Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2016: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2015: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000); Fiscal Year 2014: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 骨格筋 / タンパク質代謝 / シグナル伝達 / 筋収縮 / 翻訳調節 / mTOR / ラパマイシン / 翻訳 / 翻訳開始 / 翻訳伸長 / 運動 / レジスタンス運動 / 代謝物 / ｍTOR / ラパマイシン感受性 / Akt / PRAS40 / p70S6K / 4E-BP1 / 栄養 / 遺伝子導入 / mTORC1 / mTORC2 / rapamycin / タンパク質合成 / AZD8055 / c-Myc / リボソーム

Outline of Final Research Achievements

We investigated the molecular mechanisms of exercise-induced muscle hypertrophy. Our data suggest that exercise-induced increase in muscle protein synthesis and muscle hypertrophy are mechansitic target of rapamycin (mTOR) signaling-dependent processes. Furthermore, both rapamycin-sensitive and -insensitive mTOR (rapamycin-insensitive mTOR complex 1 (mTORC1) and mTORC2) signaling regulate these event.

Research Products

• [Int'l Joint Research] University of Wisconsin-Madison(米国)
• [Journal Article] Influence of past injurious exercise on fiber type-specific acute anabolic response to resistance exercise in skeletal muscle (2018)
  • Author(s): Ryo Takagi, Riki Ogasawara, Junya Takegaki, Arata Tsutaki, Koichi Nakazato, Naokata Ishii
  • Journal Title: Journal of Applied Physiology Volume: 124 Issue: 1 Pages: 16-22
  • DOI: 10.1152/japplphysiol.00480.2017
  • Peer Reviewed
• [Journal Article] Effects of contraction mode and stimulation frequency on electrical stimulation-induced skeletal muscle hypertrophy (2018)
  • Author(s): Yuki Ashida, Koichi Himori, Daisuke Tatebayashi, Ryotaro Yamada, Riki Ogasawara, Takashi Yamada
  • Journal Title: Journal of Applied Physiology Volume: 124 Issue: 2 Pages: 341-348
  • DOI: 10.1152/japplphysiol.00708.2017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rapamycin-insensitive mTOR regulates basal and resistance exercise-induced muscle protein synthesis (2018)
  • Author(s): Riki Ogasawara, Takeshi Suginihara
  • Journal Title: FASEB Journal Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Relationship between exercise volume and muscle protein synthesis in a rat model of resistance exercise (2017)
  • Author(s): Riki Ogasawara, Yuki Arihara, Junya Takegaki, Koichi Nakazato, Naokata Ishii
  • Journal Title: Journal of Applied Physiology Volume: 123 Issue: 4 Pages: 710-716
  • DOI: 10.1152/japplphysiol.01009.2016
  • Peer Reviewed
• [Journal Article] Repeated bouts of resistance exercise with short recovery periods activates mTOR signaling, but not protein synthesis, in mouse skeletal muscle (2017)
  • Author(s): Junya Takegaki, Riki Ogasawara, Yuki Tamura, Ryo Takagi, Yuki Arihara, Arata Tsutaki, Koichi Nakazato, Naokata Ishii
  • Journal Title: Physiological Reports Volume: 5 Issue: 22
  • DOI: 10.14814/phy2.13515
  • Peer Reviewed / Open Access
• [Journal Article] Combined effects of resistance training and calorie restriction on mitochondrial fusion and fission proteins in rat skeletal muscle (2016)
  • Author(s): Kitaoka Y, Nakazato K, Ogasawara R
  • Journal Title: Journal of Applied Physiology Volume: 121 Issue: 3 Pages: 806-8010
  • DOI: 10.1152/japplphysiol.00465.2016
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The role of mTOR signalling in the regulation of skeletal muscle mass in a rodent model of resistance exercise. (2016)
  • Author(s): Ogsawara, R., Fujita, S., Hornberger, T., Kitaoka, Y., Makanae, Y., Nakazato, K. and Ishii, N.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 31142-31142
  • DOI: 10.1038/srep31142
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Effect of electrical stimulation-induced resistance exercise on mitochondrial fission and fusion proteins in rat skeletal muscle (2015)
  • Author(s): Yu Kitaoka, Riki Ogasawara, Yuki Tamura, Satoshi Fujita, Hideo Hatta
  • Journal Title: Applied Physiology, Nutrition, and Metabolism Volume: 40 Issue: 11 Pages: 1137-1142
  • DOI: 10.1139/apnm-2015-0184
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] The role of rapamycin-insensitive mTOR signaling in the regulation of muscle protein synthesis in a rat model of resistance exercise (2017)
  • Author(s): Riki Ogasawara, Takeshi Suginihara
  • Organizer: Experimental Biology 2017
  • Int'l Joint Research
• [Presentation] Resistance training with short interval does not induce skeletal muscle hypertrophy in rats (2017)
  • Author(s): Junya Takegaki, Takaya Kotani, Riki Ogasawara, Arata Tsutaki, Koichi Nakazato, Naokata Ishii
  • Organizer: Experimental Biology 2017
  • Int'l Joint Research
• [Presentation] Visualizing spaciotemporal and quantitative dynamics of ATP levels in mouse (2017)
  • Author(s): Ishihara Y, Ohtsuki G, Miwa H, Aoki H, Tsuchida H, Ogasawara R, Sakamoto K, Yanagita M, Imamura H, Yamamoto M
  • Organizer: Cell Symposia
  • Int'l Joint Research
• [Presentation] High frequency resistance training does not cause skeletal muscle hypertrophy due to activation of proteolytic systems in rat skeletal muscle (2017)
  • Author(s): Takegaki J, Ogasawara R, Tsutaki A, Nakazato K, Ishii N
  • Organizer: Cell Symposia
  • Int'l Joint Research
• [Presentation] 骨格筋培養細胞におけるc-myc過剰発現が筋タンパク質合成に及ぼす影響 (2017)
  • Author(s): 杉野原武史, 東田一彦, 小笠原理紀
  • Organizer: 第72回日本体力医学会大会
• [Presentation] レジスタンス運動によって活性化されるラパマイシン非感受性mTORシグナルの解析 (2017)
  • Author(s): 小笠原理紀, 杉野原武史, 池田千咲, 大矢知恵梨, 松井俊憲, 松波京
  • Organizer: 第72回日本体力医学会大会
• [Presentation] セッション間の回復時間の違いが レジスタンス運動後の筋タンパク質分解系に与える影響 (2017)
  • Author(s): 竹垣淳也，小谷鷹哉，小笠原理紀，蔦木新，中里浩一，石井直方
  • Organizer: 第72回日本体力医学会大会
• [Presentation] セッション間の回復時間の違いが同強度・同回数のレジスタンス運動の効果に与える影響 (2017)
  • Author(s): 竹垣淳也，小笠原理紀，小谷鷹哉，蔦木新，中里浩一，石井直方
  • Organizer: 第25回日本運動生理学会大会
• [Presentation] 収縮様式および刺激頻度の違いが神経－筋電気刺激トレーニングによる筋肥大効果に及ぼす影響 (2017)
  • Author(s): 芦田雪, 檜森弘一, 館林大介, 山田遼太郎, 小笠原理紀, 山田崇史
  • Organizer: 第72回日本体力医学会大会
• [Presentation] レジスタンス運動による筋タンパク質合成増加におけるmTORの役割 (2017)
  • Author(s): 杉之原武史, 小笠原理紀
  • Organizer: 第21回日本体力医学会東海地方会
  • Place of Presentation: 名古屋
• [Presentation] 筋収縮様式の変化がレジスタンストレーニング適応時の筋タンパク質合成に及ぼす影響 (2016)
  • Author(s): 小笠原理紀, 對馬大介, 礒野友里絵, 杉野原武史, 丸山湧己, 中里浩一
  • Organizer: 第71回日本体力医学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-09-23
• [Presentation] mTORのATP拮抗阻害剤はラパマイシンとは異なり筋収縮による筋タンパク質合成の増加を完全に抑制する (2016)
  • Author(s): 小笠原理紀
  • Organizer: 第2回日本筋学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2016-08-05
• [Presentation] 骨格筋量調節におけるリボソーム生合成の役割 (2016)
  • Author(s): 小笠原理紀
  • Organizer: 第24回日本運動生理学会大会
  • Place of Presentation: 熊本
  • Year and Date: 2016-07-23
  • Invited
• [Presentation] 食事制限がレジスタンストレーニングによる骨格筋量とミトコンドリア関連タンパク質の変化に及ぼす影響 (2016)
  • Author(s): 小笠原理紀, 北岡祐, 中里浩一
  • Organizer: 第70回日本栄養・食糧学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2016-05-13
• [Presentation] レジスタンストレーニングによる筋肥大におけるmTORの役割 (2016)
  • Author(s): 小笠原理紀, 藤田聡
  • Organizer: 第20回日本体力医学会東海学術集会
  • Place of Presentation: 中京大学
  • Year and Date: 2016-03-13
• [Presentation] ラパマイシンはレジスタンストレーニングによる筋肥大を完全には抑制しない (2016)
  • Author(s): 小笠原理紀
  • Organizer: 第4回骨格筋生物学研究会
  • Place of Presentation: 松本大学
  • Year and Date: 2016-03-04
• [Presentation] 運動によるラパマイシン感受性mTOR活性化と骨格筋適応 (2016)
  • Author(s): 小笠原理紀
  • Organizer: 第33回筋肉の会
  • Place of Presentation: 盛岡
  • Invited
• [Presentation] 筋収縮によるmTORC1活性化はPGC-1α発現量の増加に関与しない (2015)
  • Author(s): 小笠原理紀, 北岡祐, 東田一彦, 中里浩一, 藤田聡
  • Organizer: 第70回日本体力医学会大会
  • Place of Presentation: 和歌山
  • Year and Date: 2015-09-18
• [Presentation] Effect of resistance exercise on mitochondrial fission and fusion proteins in rat skeletal muscle (2015)
  • Author(s): Kitaoka Y, Ogasawara R, Tamura Y, Fujita S, Hatta H
  • Organizer: 第70回日本体力医学会大会
  • Place of Presentation: 和歌山
  • Year and Date: 2015-09-18
• [Presentation] Resistance exercise and mitochondrial dynamics (2015)
  • Author(s): Kitaoka Y, Ogasawara R, Tamura Y, Fujita S, Hatta H
  • Organizer: Cell Symposia
  • Place of Presentation: Amsterdam, Netherlands
  • Year and Date: 2015-07-12
  • Int'l Joint Research
• [Presentation] The role of resistance exercise-induced mTORC1 activation in the regulation of protein synthesis and muscle mass. (2015)
  • Author(s): Ogasawara R, Makanae Y, Ishii N, Fujita S.
  • Organizer: Cold Spring Harbor Laboratory Meeting on Exercise Science and Health
  • Place of Presentation: Cold Spring Harbor Laboratory（ニューヨーク、アメリカ）
  • Year and Date: 2015-03-09 – 2015-03-11
• [Remarks] 小笠原理紀研究室ホームページ
  • URL: http://muscle.web.nitech.ac.jp/
• [Remarks] 研究者データベースシステム
  • URL: http://researcher.nitech.ac.jp/html/100000423_ja.html
• [Remarks] 小笠原理紀研究室
  • URL: http://muscle.web.nitech.ac.jp/
• [Remarks] 名古屋工業大学研究者データベース
  • URL: http://researcher.nitech.ac.jp/html/100000423_ja.html