| Budget Amount *help |
¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Outline of Final Research Achievements |
We revealed the biogenesis and physiological function of 5-formylcytidien (f5C) modification specifically found in the wobble position of human/vertebrates mitochondrial tRNA-Met. A metabolic labeling analysis enables us to predict that f5C synthesis initiates methylation of C to 5-methylcitidine (m5C), and then oxidizes m5C to f5C. Actually, we successfully identified the methyltransferase NSUN3 and the oxygenase ALKBH1 and performed in vitro f5C reconstitution by using these enzymes. Knockout cells of each gene exhibited mitochondrial dysfunction and decreased mitochondrial translation in biochemical analyses. As for species-specific modifications other than f5C, in vitro reconstitution or modifying gene identification were achieved. To deeply understand the function of these modifications, further investigations will be required.
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