in vivo RNA Imaging Toward Mapping the Dynamics of Gene Expression in the Plastic Brain
Project/Area Number |
26702038
|
Research Category |
Grant-in-Aid for Young Scientists (A)
|
Allocation Type | Partial Multi-year Fund |
Research Field |
Basic / Social brain science
|
Research Institution | Kyoto University |
Principal Investigator |
Wang Dan Ohtan 京都大学, 高等研究院, 特定拠点准教授 (50615482)
|
Research Collaborator |
OOMOTO Ikumi
MATSUO Naoki
OKAMOTO Akimitsu
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000)
Fiscal Year 2016: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2014: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
|
Keywords | RNA imaging / synapse / neurodevelopment / RNA dynamics / motor cortex / virtual reality / spatial regulation / simulation / mathematical models / in vivo / real-time / spatiotemporal / mechanism / RNA methylation / RNA / foci dynamics / transcription |
Outline of Final Research Achievements |
With this support, we aimed to study the dynamics of RNA and their regulation as the molecular basis of long-term memory. By introducing a hybridization-sensitive fluorescent probe into intact animals and taking measurement which was previously impossible, we succeeded in visualizing RNA molecules at the level of single cells in vivo and to measure simultaneous changes in hundreds of cells (NAR, 2015). Furthermore, we discovered a novel mechanism of neuronal transcription factors that is not only required for gene activation but for suppressing the function of cognate microRNA. As the result, this mechanism prevents the functionally related gene products from being degraded (Front Mol. Neurosci, 2017). Finally, we defined a new layer of post-transcriptional regulation at neuronal synapses that are enriched in neurodevelopment and neuropsychiatric pathways (Nat Neurosci, in press) .
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Report
(5 results)
Research Products
(47 results)
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[Journal Article] Transport Granules Bound with Nuclear Cap Binding Protein and Exon Junction Complex Are Associated with Microtubules and Spatially Separated from eIF4E Granules and P Bodies in Human Neuronal Processes2017
Author(s)
Wang DO, Ninomiya K, Mori C, Koyama A, Haan M, Kitabatake M, Hagiwara M, Chida K, Takahashi SI,Ohno M, Kataoka N
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Journal Title
frontiers in Molecular Biosciences
Volume: 4
Pages: 1-12
Related Report
Peer Reviewed / Open Access
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[Journal Article] ECHO-liveFISH: in vivo RNA Labeling Reveals Dynamic Regulation of Nuclear RNA Foci in Living Tissues2015
Author(s)
Oomoto I, Hirano-Suzuki A, Umeshima H, Han YW, Yanagisawa H, Carlton P, Harada Y, Kengaku M, Okamoto A, Shimogori T, and Wang DO.
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Journal Title
Nucl Acids. Res
Volume: 43
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Live-cell Imaging of Endogenous mRNAs with a Small Molecule2015
Author(s)
Sato, S.*, Watanabe, M., Katsuda, Y., Murata, A., Wang, D. O., Uesugi M.
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Journal Title
Angew. Chem. Int. Ed.
Volume: 54
Issue: 6
Pages: 1855-1858
DOI
NAID
Related Report
Peer Reviewed
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