| Budget Amount *help |
¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2014: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
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| Outline of Final Research Achievements |
In this study, we established technology base for the proof-of-concept of a new technology called digital nanochannel (DN), which was proposed by us and holds great potential allowing single cell omics analysis at the single molecule level. First, the basic technology for fabricating nanofluidic chips with precise, high density, stable DN structures was developed. Second, the mechanism of signal detection for single molecule counting by DN was confirmed. Third, fundamental technologies to improve the performance of single molecule detection by DN, such as suppression of nonspecific protein adsorption in DN and regeneration of DN chips, were further established. These results promise that DN could be a powerful tool for single cell omics studies after further development in the future.
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