Comprehensive spectroscopic study on the transport mechanism of sodium pump rhodopsin
| Project/Area Number |
26708001
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Physical chemistry
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
Inoue Keiichi 名古屋工業大学, 工学(系)研究科(研究院), 准教授 (90467001)
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| Research Collaborator |
Kandori Hideki 名古屋工業大学, 大学院工学研究科, 教授 (70202033)
Yoshizumi Rei
Kato Yoshitaka
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
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| Keywords | 微生物型ロドプシン / ナトリウムポンプ / レチナール / プロトン移動 / 光反応サイクル / フラッシュフォトリシス / X線結晶構造解析 / オプトジェネティクス / ロドプシン / 分光法 / 海洋性細菌 / 生物物理化学 / ラマン分光 |
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| Outline of Final Research Achievements |
In this study, we studied the Na+ transport mechanism of light-driven Na+ pump rhodopsin (NaR) by functional assay of amino-acid mutant, flash photolysis, FTIR and X-ray crystallographic analysis. The results revealed that the opening of the gate for Na+ uptake with H+ transfer from the Schiff-base of chromophore retinal to Asp116 after photo-excitation, and the following Na+ binding in the protein is important for Na+-transport function. The site-directed mutation of Na+-uptake site whose structure was revealed in the crystallographic analysis achieved the creation of novel K+ pump and Cs+ pumps. Furthermore, we identified new NaRs which show photocurrents more than two-times higher in mammalian cells compared to the previously reported NaR.
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Report
(4 results)
Research Products
(155 results)
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[Journal Article] A natural light-driven inward proton pump2016
Author(s)
Keiichi Inoue, Shota Ito, Yoshitaka Kato, Yurika Nomura, Mikihiro Shibata, Takayuki Uchihashi, Satoshi P. Tsunoda and Hideki Kandori
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Journal Title
Nature Communications
Volume: 7
Issue: 1
Pages: 13415-13415
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Structural basis for Na+ transport mechanism by a light-driven Na+ pump2015
Author(s)
H. E. Kato, K. Inoue, R. Abe-Yoshizumi, Y. Kato, H. Ono, M. Konno, S. Hososhima, T. Ishizuka, M. R. Hoque, H. Kunitomo, J. Ito, S. Yoshizawa, K. Yamashita, M. Takemoto, T. Nishizawa, R. Taniguchi, K. Kogure, A. D. Maturana, Y. Iino, H. Yawo, R. Ishitani, H. Kandori*, O. Nureki*
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Journal Title
Nature
Volume: 未定
Issue: 7550
Pages: 48-53
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] 1.Structural Basis for Na+ Transport Mechanism by a Light-Driven Na+ Pump2015
Author(s)
H. E. Kato, K. Inoue, R. Abe-Yoshizumi, Y. Kato, H. Ono, M. Konno, T. Ishizuka, M. R. Hoque, S. Hososhima, H. Kunitomo, J. Ito, S. Yoshizawa, K. Yamashita, M. Takemoto, T. Nishizawa, R. Taniguchi, K. Kogure, A. D. Maturana, Y. Iino, H. Yawo, R. Ishitani, H. Kandori, O. Nureki
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Journal Title
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] "Natural light-driven inward proton pump"2016
Author(s)
K. Inoue, S. Ito, Y. Kato, Y. Nomura, M. Shibata, T. Uchihashi, S. P. Tsunoda, H. Kandori
Organizer
The 54th Annual Meeting of the Biophysical Society of Japan
Place of Presentation
Tsukuba
Year and Date
2016-11-25
Related Report
Int'l Joint Research
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[Presentation] Mechanisms of two different function on KR22015
Author(s)
M. R. Hoque, S. Hososhima, K. Yoshida, T. Ishizuka, K. Inoue, H. Kandori, H. Yawo
Organizer
The 38th Annual Meeting of the Japan Neuroscience Society
Place of Presentation
Kobe, Japan
Year and Date
2015-07-28
Related Report
Int'l Joint Research
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