|Budget Amount *help
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2014: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
|Outline of Final Research Achievements
For elucidating molecular mechanisms underlying functions of membrane proteins such as ion channels and transporters, I engaged in development of a new infrared spectroscopic technique which enables quantitative measurement of interactions between protein and ion or substrate and time-resolved measurement of structural changes of protein upon their functioning. Slow or fast buffer-exchange system was incorporated in an infrared spectrometer with a configuration of attenuated total reflection. I succeeded to identify an infrared absorption band as “molecular fingerprint” for the ion selectivity filter which realizes high selectivity for potassium ions in potassium channel, and to measure changes of the infrared band reflecting structural changes of the selectivity filter upon binding of potassium ions in time-resolved manner.