Instrumentation of high-throughput Raman optical activity spectrometer and structural anaylysis of naturing amyloid fibril
| Project/Area Number |
26708017
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
Yamamoto Shigeki 大阪大学, 理学(系)研究科(研究院), 助教 (60552784)
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| Research Collaborator |
OZAKI Yukihiro
BOUŘ Petr
TSUKAHARA Satoshi
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥21,190,000 (Direct Cost: ¥16,300,000、Indirect Cost: ¥4,890,000)
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| Keywords | アミロイド / 二次構造 / 振動光学活性 / 分子断片化法 / インスリン / ラマン光学活性 / アミロイド線維 / βシート構造 / キラリティ / キラル振動分光 / 分子動力学 / 量子力学 / 分光分析 / 分析化学 / スペクトル分析 |
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| Outline of Final Research Achievements |
Raman Optical Activity (ROA) is a vibrational spectroscopy sensitive to molecular conformation in solutions, which can be a new analytical method for protein structures in solution phase. However, there has been tasks to solve in ROA, a lack of standard way of interpretation of amyloid fibril proteins and a low sensitivity of the instrument. We targeted on a protein insulin as a good model of amyloid fibril-forming proteins and analyzed its second- and higher-order proteins structures of the amyloid. On the basis of comparisons of experimental and calculated ROA spectra, we could determine the stable insulin structure in the fibril as beta-roll structure, not beta-helical one. The calculation was done by combining molecular dynamics simulation and quantum mechanical simulation of the spectra aided with a fragment method. We developed a new ROA instrument with multi-lens setup which brings 10-times stronger Raman signal compared to conventional back-scattered one.
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Report
(4 results)
Research Products
(11 results)
