Growth inhibition of Pseudomonas aeruginosa by HasA with metal-phthalocyanine and a novel elimination system of bacteria by photo-irradiation
Project/Area Number |
26708018
|
Research Category |
Grant-in-Aid for Young Scientists (A)
|
Allocation Type | Partial Multi-year Fund |
Research Field |
Bio-related chemistry
|
Research Institution | Nagoya University |
Principal Investigator |
Shoji Osami 名古屋大学, 理学研究科, 准教授 (90379587)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥24,830,000 (Direct Cost: ¥19,100,000、Indirect Cost: ¥5,730,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
|
Keywords | ヘム / 緑膿菌 / 増殖阻害 / フタロシアニン / ポルフィリン / 鉄 / 光照射 / 一重項酸素 / 細菌 / 蛋白質 / ヘム獲得蛋白質 / 金属錯体 / 光殺菌 / 酵素 / 殺菌 / ヘム獲得蛋白 / 結晶構造解析 |
Outline of Final Research Achievements |
HasA (Heme acquisition system A) is a hemophore secreted by some pathogenic bacteria having a heme acquisition system (Has system) such as Pseudomonas aeruginosa. We have found that HasA can capture several synthetic metal complexes other than heme such as iron-salophen and iron-phthalocyanine. The crystal structures of HasA harboring iron-salophen and iron-phthalocyanine showed only small structural perturbations compared with that of HasA with heme. We also found that HasA bound to iron-phthalocyanine strongly inhibited HasA-mediated heme acquisition. Furthermore, we have demonstrated that Pseudomonas aeruginosa can be eliminated by photo-irradiation at 680 nm in the presence of HasA bound to gallium-phthalocyanine due to generation of singlet oxygen.
|
Report
(5 results)
Research Products
(34 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Growth Inhibition of Pseudomonas Aeruginosa by Hasa with Synthetic Metal Complexes and A Novel Elimination System of Bacteria by Photo-Irradiation2016
Author(s)
O. Shoji, C. Shirataki, Y. Shisaka, H. Uehara, Y. Iwai, A. Nakashima, M. Terada, S. Ozaki, H. Sugimoto, Y. Shiro, Y. Watanabe
Organizer
AsBIC8
Place of Presentation
Auckland, Newzealand
Year and Date
2016-12-04
Related Report
Int'l Joint Research / Invited
-
-
-
-
-
-
-
-
-
-
-
-
-