High-sensitivity biological solid-state NMR utilizing general photo-chemically induced transient polarization
| Project/Area Number |
26708019
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
Matsuki Yoh 大阪大学, たんぱく質研究所, 助教 (70551498)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | 固体NMR / 動的核分極 / 光CIDNP / 感度向上 / 固体NMRの感度向上 / 動的核分極法 / 光励起 |
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| Outline of Final Research Achievements |
Magic-angle spinning solid-state NMR is a valid technique for structural and dynamical studies of non-crystalline/insoluble molecular systems such as membrane proteins at atomic resolution, but suffers from its low sensitivity. A number of nuclear hyper-polarization techniques using the high electron polarization is known, but limited by their own limitations. This work focuses on the so-far under-exploited “solid-state CIDNP" phenomenon based on the photo-chemically induced transient electron polarization, and has developed a novel polarizing agents that do not rely on the protein scaffold required for the conventional solid-state CIDNP experiments. This would be the basis for the first generic and chemically pure polarizing agent for solid-state CIDNP that only uses ordinary small organic molecules, which is easy to store/handle. In addition, a novel organic glass matrix that is hard and efficient for distribution of the local polarization to the bulk sample has also been developed.
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Report
(4 results)
Research Products
(9 results)
