Research Project
Grant-in-Aid for Young Scientists (A)
I aim to construct a method to determine position and concentration of a specific mRNA in a live cell. We tried to select and utilize the aptamers for two compounds: ruthenium trisbipyridine whose photo-exited state possesses strong redox ability and aziderhodamine which emits fluorescence after reduction to amino rhodamine by the photo-exited ruthenium trisbipridine. We have found that the aptamer which binds ruthenium trisbipyridine elongated the lifetime of the photo-exited ruthenium tirsbipyridine 4 fold by reducing oxygen quenching. Although we have tried 30 candidate RNA obtaind by the selection for aziderhodamine, none of them exhibit a reproducible positive result.
All 2016 2014
All Presentation (5 results)
