in cell Templated Reaction by employing in vitro selected RNA aptamers
| Project/Area Number |
26708020
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
UZAWA Takanori 国立研究開発法人理化学研究所, 伊藤ナノ医工学研究室, 専任研究員 (60554376)
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| Project Period (FY) |
2014-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥25,870,000 (Direct Cost: ¥19,900,000、Indirect Cost: ¥5,970,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥24,700,000 (Direct Cost: ¥19,000,000、Indirect Cost: ¥5,700,000)
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| Keywords | アプタマー / ルテニウム錯体 / エネルギー移動 / 電子移動 / アジドローダミン / 進化分子工学 |
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| Outline of Final Research Achievements |
I aim to construct a method to determine position and concentration of a specific mRNA in a live cell. We tried to select and utilize the aptamers for two compounds: ruthenium trisbipyridine whose photo-exited state possesses strong redox ability and aziderhodamine which emits fluorescence after reduction to amino rhodamine by the photo-exited ruthenium trisbipridine. We have found that the aptamer which binds ruthenium trisbipyridine elongated the lifetime of the photo-exited ruthenium tirsbipyridine 4 fold by reducing oxygen quenching. Although we have tried 30 candidate RNA obtaind by the selection for aziderhodamine, none of them exhibit a reproducible positive result.
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Report
(3 results)
Research Products
(5 results)
