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Light-assisted liquid chromatography of proteins using carbon nanotubes

Research Project

Project/Area Number 26708021
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Bio-related chemistry
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Hirano Atsushi  国立研究開発法人産業技術総合研究所, ナノ材料研究部門, 主任研究員 (90613547)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2016: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2015: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Keywordsナノチューブ / 蛋白質 / ナノチューブ・フラーレン
Outline of Final Research Achievements

A liquid chromatography column using carbon nanotubes as ligands was fabricated for realizing light-assisted liquid chromatography for protein separation. It was found that the carbon nanotubes immobilized in the column interact with aromatic amino acids, especially with tryptophan, and that they oxidize cysteine, resulting in disulfide formation. Thus, prevention of excess protein adsorption onto the carbon nanotubes and suppression of cysteine oxidation are necessary for fine control of the protein separation in this system.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (10 results)

All 2017 2016 2015 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (4 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Disulfide bond formation of thiols by using carbon nanotubes2017

    • Author(s)
      Atsushi Hirano, Tomoshi Kameda, Shun Sakuraba, Momoyo Wada, Takeshi Tanaka, Hiromichi Kataura
    • Journal Title

      Nanoscale

      Volume: 9 Issue: 17 Pages: 5389-5393

    • DOI

      10.1039/c7nr01001j

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Arginine Suppresses the Adsorption of Lysozyme onto Single-wall Carbon Nanotubes2016

    • Author(s)
      Kazuki Iwashita, Kentaro Shiraki, Rieko Ishii, Takeshi Tanaka, Atsushi Hirano
    • Journal Title

      Chemistry Letters

      Volume: 45 Issue: 8 Pages: 952-954

    • DOI

      10.1246/cl.160390

    • NAID

      130005254518

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Origin of the Surfactant-Dependent Redox Chemistry of Single-Wall Carbon Nanotubes2016

    • Author(s)
      Atsushi Hirano, Tomoshi Kameda, Yohei Yomogida, Momoyo Wada, Takeshi Tanaka, Hiromichi Kataura
    • Journal Title

      ChemNanoMat

      Volume: 2 Issue: 9 Pages: 911-920

    • DOI

      10.1002/cnma.201600190

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Liquid Chromatographic Analysis of the Interaction between Amino Acids and Aromatic Surfaces Using Single-Wall Carbon Nanotubes2015

    • Author(s)
      Kazuki Iwashita, Kentaro Shiraki, Rieko Ishii, Takeshi Tanaka, Atsushi Hirano
    • Journal Title

      Langmuir

      Volume: 31 Issue: 32 Pages: 8923-8929

    • DOI

      10.1021/acs.langmuir.5b02500

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 単層カーボンナノチューブによるチオールのジスルフィド結合形成2016

    • Author(s)
      平野 篤, 和田 百代, 田中 丈士, 片浦 弘道
    • Organizer
      第50回 フラーレン・ナノチューブ・グラフェン 総合シンポジウム
    • Place of Presentation
      東京大学 伊藤国際学術研究センター 伊藤謝恩ホール
    • Year and Date
      2016-02-22
    • Related Report
      2015 Annual Research Report
  • [Presentation] Inhibition of protein adsorption onto carbon nanotubes by arginine(アルギニンによるカーボンナノチューブへの蛋白質吸着の抑制)2016

    • Author(s)
      平野 篤、岩下 和輝、白木 賢太郎、石井 梨恵子、田中 丈士
    • Organizer
      第16回日本蛋白質科学会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2016 Annual Research Report
  • [Presentation] 水溶液中におけるカーボンナノチューブとアミノ酸の相互作用2016

    • Author(s)
      平野 篤、岩下 和輝、白木 賢太郎、桜庭 俊、亀田 倫史、石井 梨恵子、田中 丈士
    • Organizer
      第五回ナノカーボンバイオシンポジウムプログラム
    • Place of Presentation
      北海道立道民活動センター かでる2・7(北海道)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Interactions between carbon nanotubes and aromatic amino acids2016

    • Author(s)
      平野 篤、岩下 和輝、白木 賢太郎、桜庭 俊、亀田 倫史、石井 梨恵子、田中 丈士
    • Organizer
      第51回 フラーレン・ナノチューブ・グラフェン 総合シンポジウム
    • Place of Presentation
      北海道立道民活動センター かでる2・7(北海道)
    • Related Report
      2016 Annual Research Report
  • [Remarks] 代表者のWebページ

    • URL

      https://staff.aist.go.jp/hirano-a/index.html

    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] カーボンナノチューブを用いたジスルフィド結合形成反応2016

    • Inventor(s)
      平野 篤、田中 丈士、片浦 弘道
    • Industrial Property Rights Holder
      平野 篤、田中 丈士、片浦 弘道
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-029888
    • Filing Date
      2016-02-19
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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