Light-assisted liquid chromatography of proteins using carbon nanotubes
| Project/Area Number |
26708021
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Partial Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Hirano Atsushi 国立研究開発法人産業技術総合研究所, ナノ材料研究部門, 主任研究員 (90613547)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2016: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2015: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Keywords | ナノチューブ / 蛋白質 / ナノチューブ・フラーレン |
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| Outline of Final Research Achievements |
A liquid chromatography column using carbon nanotubes as ligands was fabricated for realizing light-assisted liquid chromatography for protein separation. It was found that the carbon nanotubes immobilized in the column interact with aromatic amino acids, especially with tryptophan, and that they oxidize cysteine, resulting in disulfide formation. Thus, prevention of excess protein adsorption onto the carbon nanotubes and suppression of cysteine oxidation are necessary for fine control of the protein separation in this system.
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Report
(4 results)
Research Products
(10 results)
