Creation of Highly Sensitive SERS Particles for Visualization of Chemical Reactions in Cells

• Project/Area Number: 26708025
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Polymer/Textile materials
• Research Institution: Tohoku University
• Principal Investigator: YABU HIROSHI 東北大学, 材料科学高等研究所, 准教授 (40396255)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 自己組織化 / 微粒子 / 有機-無機ハイブリッド / ラマン散乱 / 細胞 / ラマン計測 / 細胞培養 / プラズモン / コンポジット

Outline of Final Research Achievements

By using the original self-organized precipitation (SORP) method, core-shell particles comprising of polystyrene core and amino-terminated polybutadiene shell have been prepared. Since obtained particles have positive surface charges, composite particles having gold nanoparticle clusters were successfully prepared by mixing negatively charged gold nanoparticle dispersions with core-shell particles. These particles show strong surface enhanced Raman scattering signals by irradiation of near infrared light excitation. Furthermore, magnetic responsive composite particles were also prepared by introducing magnetic nanoparticles into the composite particles.

Research Products

• [Int'l Joint Research] University of Lincoln(United Kingdom)
• [Journal Article] Phase behavior in three-dimensional confinement spaces of symmetrical diblock copolymers: experimental observation and theoretical simulation of multi-pod structures (2016)
  • Author(s): T. Higuchi, M. Pina, A. Zvelindovsky, H. Jinnai, H. Yabu
  • Journal Title: Journal of Polymer Science Part B: Polymer Physics Volume: 54 Issue: 17 Pages: 1702-1709
  • DOI: 10.1002/polb.24072
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Thermally Stable Honeycomb-Patterned Porous Films of a Poly(L-Lactic Acid) and Poly(D-Lactic Acid) Stereo Complex Prepared Using the Breath Figure Technique (2016)
  • Author(s): H. Abe, Y. Hirai, H. Yabu
  • Journal Title: Macromolecular Materials & Engineering Volume: 301 Pages: 523-529
  • Peer Reviewed
• [Journal Article] Frustrated phases under three-dimensional confinement simulated by a set of coupled Cahn-Hilliard equations (2016)
  • Author(s): Edgar Avalos, Takeshi Higuchi, Takashi Teramoto, Hiroshi Yabu and Yasumasa Nishiura
  • Journal Title: Soft Matter Volume: 27 Issue: 27 Pages: 5905-5914
  • DOI: 10.1039/c6sm00429f
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Proton Conductivities of Lamellae-forming Bio-inspired Block Copolymer Thin Films Containing Silver Nanoparticles (2016)
  • Author(s): H. Yabu, J. Matsui, M. Hara, S. Nagano, Y. Matsuo, Y. Nagao
  • Journal Title: Langmuir Volume: 32 Issue: 37 Pages: 9484-9491
  • DOI: 10.1021/acs.langmuir.6b02521
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] One-Pot Preparation of Organic–Inorganic Composite Microspheres Comprising Silica Nanoparticles and End-Functionalized Polymers (2016)
  • Author(s): Hiroki Satoh, Hiroshi Yabu
  • Journal Title: Macromolecular Materials and Engineering Volume: 301 Issue: 3 Pages: 279-286
  • DOI: 10.1002/mame.201500338
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] “Silver Nanoparticle Arrays Prepared by In Situ Automatic Reduction of Silver Ions in Mussel-Inspired Block Copolymer Films” (2016)
  • Author(s): Saito, Y; Higuchi, T; Jinnai, H; Hara, M; Nagano, S; Matsuo, Y; Yabu, H;
  • Journal Title: MACROMOLECULAR CHEMISTRY AND PHYSICS Volume: 217 Issue: 6 Pages: 726-734
  • DOI: 10.1002/macp.201500504
  • Peer Reviewed
• [Journal Article] Fabrication of NIR-Excitable SERS-Active Composite Particles Composed of Densely Packed Au Nanoparticles on Polymer Microparticles (2015)
  • Author(s): M. Kanahara, H. Sato, T. Higuchi, A. Takahara, H. Jinnai, K. Harano, S. Okada, E. Nakamura, Y. Matsuo, H. Yabu
  • Journal Title: Particle & Particle Systems Characterization Volume: 4 Issue: 4 Pages: 441-447
  • DOI: 10.1002/ppsc.201400191
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Nanostructured Polymer Particles for Display Applications and Others Prepared by Self-Organization Processes (2016)
  • Author(s): 藪浩
  • Organizer: IDW/AD'16
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2016-12-08
  • Int'l Joint Research / Invited
• [Presentation] ナノミセルと UV 光照射による金属フタロシアニンナノ結晶の合成 (2016)
  • Author(s): 藪浩
  • Organizer: 有機分子・バイオエレクトロニクス分科会研究会
  • Place of Presentation: 天童ホテル
  • Year and Date: 2016-06-09
• [Presentation] Creation of Functional Organic-Inorganic Composite Particles by Self-Organization Process (2014)
  • Author(s): Hiroshi Yabu
  • Organizer: IUMRS-ICA2014
  • Place of Presentation: 福岡大学
  • Year and Date: 2014-08-24 – 2014-08-30
• [Presentation] Creation of Functional Organic-Inorganic Composite Particles by Self-Organization Process (2014)
  • Author(s): Hiroshi Yabu
  • Organizer: MASA
  • Place of Presentation: 筑波
  • Year and Date: 2014-07-18 – 2014-07-19
  • Invited