| Budget Amount *help |
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Outline of Final Research Achievements |
Over 99% of microorganisms in environment remain uncultured. However, very few attempts have been carried out for development of new microbial cultivation technology so far. The objective of the study is development of new cultivation methods based on a new concept as “designing miniscule space”. First, a fully automated microbial cultivation method for isolating pure bacterial cultures “a microbial trap” was developed. This new approach can automatically isolate individual bacterial species from heterogeneous populations, In this study, we successfully applied “microbial trap” to environmental sample for isolating bacteria. Second, we established a new microbial cultivation platform providing extremely high inoculum cell density. Hydro-gel particles (10 - 30 um in diameter) entrapping single cells with medium softly aggregated in oil is the key structure. This method significantly increases the cultivation efficiencies compared with the conventional method.
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