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Miniscule designing: innovation for microbial cultivation

Research Project

Project/Area Number 26709063
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionHiroshima University

Principal Investigator

Aoi Yoshiteru  広島大学, 先端物質科学研究科, 准教授 (40386636)

Research Collaborator Goluch Ed  
Project Period (FY) 2014-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywordsナノ・マイクロ成型技術 / 微小ゲル粒子 / マイクロカプセル / 微生物間相互作用 / 分離・培養 / 難培養 / 未培養微生物 / ナノファブリケーション / 分離培養 / ゲルマイクロドロップレット / エマルジョン / マイクロ・ナノデバイス / 環境微生物 / マイクロデバイス / 微生物 / 培養
Outline of Final Research Achievements

Over 99% of microorganisms in environment remain uncultured. However, very few attempts have been carried out for development of new microbial cultivation technology so far. The objective of the study is development of new cultivation methods based on a new concept as “designing miniscule space”. First, a fully automated microbial cultivation method for isolating pure bacterial cultures “a microbial trap” was developed. This new approach can automatically isolate individual bacterial species from heterogeneous populations, In this study, we successfully applied “microbial trap” to environmental sample for isolating bacteria. Second, we established a new microbial cultivation platform providing extremely high inoculum cell density. Hydro-gel particles (10 - 30 um in diameter) entrapping single cells with medium softly aggregated in oil is the key structure. This method significantly increases the cultivation efficiencies compared with the conventional method.

Academic Significance and Societal Importance of the Research Achievements

地球上には、膨大な数・種類の微生物が存在しているが、それら環境中の微生物の99%以上は未だ培養できないことが知られている。このため、環境微生物の正しい理解に基づく「制御」や「バイオリソースとしての開拓」は、幅広い分野における重要課題であるにも関わらず, そのほとんどが未解明・未利用のまま残されている。一方で、従来法に替わる分離培養手法はほとんど登場していない。したがって、本研究の成果である、新しいコンセプトに基づく新規分離培養手法の開発(分離培養法の革新)は、「環境微生物から得られる情報の質,量」,さらに「バイオリソースとしての可能性」を飛躍的に向上させるブレークスルーになると考えられる。

Report

(6 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (20 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (15 results) (of which Int'l Joint Research: 6 results,  Invited: 7 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Northeastern University(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Northeastern University(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Northeastern University(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] 培養できない微生物とは?どうしたら培養できるのか?培養手法の革新2016

    • Author(s)
      青井議輝
    • Journal Title

      環境バイオテクノロジー学会誌

      Volume: 16 Pages: 59-64

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] Why does growing "in situ" allows recovery of fastidious microbial types from nature?2018

    • Author(s)
      Dawoon Jung, Koshi Machida, Yoichi Nakao, Tomonori Kindaichi, Akiyoshi Ohashi, Yoshiteru Aoi
    • Organizer
      日本微生物生態学会32回大会/10th ASME
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Fully automated microbial cultivation technique by Nano-constriction2018

    • Author(s)
      Dawoon Jung, Yoshiteru Aoi
    • Organizer
      The 3rd International Symposium on Biomedical Engineering
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Miniscule space designing for microbial cultivation2018

    • Author(s)
      Yoshiteru Aoi
    • Organizer
      日本微生物生態学会第32回大会/10th ASME
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] なぜ多くの微生物は培養困難なのか?休眠・覚醒現象から迫る2018

    • Author(s)
      青井議輝
    • Organizer
      バイオインダストリー協会”未来へのバイオ技術”勉強会、微生物・覚醒
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Multiple advanced cultivation techniques for isolation of fastidious microorganisms associated with the marine sponge2017

    • Author(s)
      Dawoon Jung, Koshi Machida, Yoichi Nakao, Tomonori Kindaichi, Akiyoshi Ohashi, Yoshiteru Aoi
    • Organizer
      The 9th Asian Symposium on Microbial Ecology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 難培養性微生物とは何か?どうしたら培養できるのか?2017

    • Author(s)
      青井 議輝
    • Organizer
      日本臨床腸内微生物学会総会・学術集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 培養手法の革新:難培養性微生物の正体と資源としての可能性2017

    • Author(s)
      青井 議輝
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 環境中の微生物を自動的に“捕え”て“分離”する革新的分離培養手法の開発2017

    • Author(s)
      植田 雄人, Tandogan N, Goluch E, 金田 一智規,大橋 晶良,青井 議輝
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 微生物を自動的に“捕え”て“分離”する革新的分離培養手法2016

    • Author(s)
      植田雄人, Dawoon Jung, Nil Tandogan, Edger D Goluch , 金田一智規, 大橋晶良, 青井議輝
    • Organizer
      日本微生物生態学会第31回大会
    • Place of Presentation
      横須賀
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] 分離培養手法の革新-難培養性微生物の正体と可能性2016

    • Author(s)
      青井議輝
    • Organizer
      第28回微生物シンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2016-09-02
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Letting microorganisms escape from loneliness and grow2016

    • Author(s)
      Yoshiteru Aoi, Yuki Takagi, Tomonori Kindaichi, Akiyoshi Ohashi
    • Organizer
      16th International Symposium on Microbial Ecology
    • Place of Presentation
      モントリオール(カナダ)
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] An innovative cultivation method that enable isolating pure culture automatically2016

    • Author(s)
      5.Yuto Ueda, Nil Tandogan, Edgar Goluch, Tomonori Kindaichi, Akiyoshi Ohashi1, Yoshiteru Aoi
    • Organizer
      16th International Symposium on Microbial Ecology
    • Place of Presentation
      モントリオール(カナダ)
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 培養できない微生物の正体と培養手法の革新2016

    • Author(s)
      青井議輝
    • Organizer
      環境バイオテクノロジー学会2016
    • Place of Presentation
      広島
    • Year and Date
      2016-06-13
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Letting microorgasnism escape loneliness and grow2015

    • Author(s)
      Yuki Tagagi,Tomonori Kindaichi, Akiyoshi Ohashi, Yoshiteru Aoi
    • Organizer
      第30回日本微生物生態学会
    • Place of Presentation
      土浦
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 培養困難な微生物の獲得とその意義2015

    • Author(s)
      青井議輝
    • Organizer
      第18回日本水環境学会シンポジウム
    • Place of Presentation
      長野
    • Year and Date
      2015-09-14
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Patent(Industrial Property Rights)] 微生物の分離培養方法及び凝集体含有溶液2015

    • Inventor(s)
      青井議輝、高木雄貴、大橋晶良
    • Industrial Property Rights Holder
      青井議輝、高木雄貴、大橋晶良
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-196882
    • Filing Date
      2015-10-02
    • Related Report
      2015 Annual Research Report

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Published: 2014-04-04   Modified: 2023-03-23  

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