Immunogenetic Repertoire Analysis for Tumor-infiltrating Lymphocytes - toward the Understanding of Tumor Immunology -

• Project/Area Number: 26710009
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Tumor therapeutics
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Katoh Hiroto 東京医科歯科大学, 難治疾患研究所, 助教 (60446549)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000); Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2014: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: 免疫ゲノム / ゲノム解析 / 腫瘍免疫 / 抗原受容体シーケンス

Outline of Final Research Achievements

The purpose of this study was to comprehensively analyze the antigen receptor repertoires of tumor-infiltrating lymphocytes by the next generation sequencing and to elucidate the global picture of tumor immunity from the viewpoint of immuno-genetics. Additionally, I also aimed to create a basic database to develop new therapeutic strategies against cancers, such as novel anti-cancer antibodies, based on antigen receptor repertoire sequences that existed dominantly in cancer tissues. The following achievements were obtained.
(1) Next generation sequencing protocols for the antigen receptor repertoires of tumor-infiltrating lymphocytes in clinical cancer tissues were established. (2) A number of T cell receptors and immunoglobulins that were specifically and dominantly infiltrated in cancer tissues were identified. (3) Functional screening of multiple cancer specific immunoglobulin molecules reconstructed by artificial expression system could be carried out.

Research Products

• [Journal Article] Genomic pathobiology of gastric carcinoma. (2017)
  • Author(s): Katoh H, Ishikawa S.
  • Journal Title: Pathol Int. Volume: 67 Issue: 2 Pages: 63-63
  • DOI: 10.1111/pin.12493
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 胃がん領域のゲノム病理学の進歩とゲノム個別化がん治療 (2016)
  • Author(s): 加藤洋人、石川俊平
  • Journal Title: 血液内科 Volume: 72(4) Pages: 544-549
  • NAID: 40020854823
• [Presentation] Prediction of Antigen-Specific Immunoglobulins from Amino Acid Sequences using Semi-Supervised Deep Learning. (2016)
  • Author(s): Hiroki Konishi, Daisuke Komura, Hiroto Katoh, Ken Tominaga, Ryohei Suzuki, Shumpei Ishikawa.
  • Organizer: 15th European Conference on Computational Biology
  • Place of Presentation: Hague, Netherlands
  • Year and Date: 2016-09-03
  • Int'l Joint Research
• [Presentation] Immunogenetic Characterization of Tumor Infiltrating TCR Repertoire in the Gastric Carcinoma Environments Using Archived Histopathological Specimens. (2016)
  • Author(s): Hiroto Katoh, Daisuke Komura, Hiroki Konishi, Ryohei Suzuki, Asami Yamamoto, Reiko Sato, Masashi Fukayama, Hiroyuki Aburatani, Shumpei Ishikawa.
  • Organizer: AIRR 2016 Community Meeting
  • Place of Presentation: Rockville, MD, USA
  • Year and Date: 2016-06-27
  • Int'l Joint Research
• [Presentation] Immunogenomic Characterization of Tumor Infiltrating TCR Repertoire in the Gastric Carcinoma Enviroments using Archived Histopathological Specimens (2015)
  • Author(s): H. Katoh, D. Komura, H. Konishi, A. Yamamoto, M. Fukayama, S. Ishikawa
  • Organizer: 3rd Annual immungenomics 2015 Shaping the Future of Human Health
  • Place of Presentation: Huntsville, Alabama, USA
  • Year and Date: 2015-09-28
  • Int'l Joint Research
• [Presentation] IMMUNOGENETIC PROFILING OF TUMOR INFILTRATING T CELLS AMONG HUMAN GASTRIC CANCER (2015)
  • Author(s): H. Katoh, D. Komura, H. Konishi, A. Yamamoto, M. Fukayama, S. Ishikawa
  • Organizer: IMMUNE PROFILING IN HEALTH AND DISEASE
  • Place of Presentation: Seattle, Washington, USA
  • Year and Date: 2015-09-09
  • Int'l Joint Research