| Budget Amount *help |
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2014: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Outline of Final Research Achievements |
The purpose of this study was to comprehensively analyze the antigen receptor repertoires of tumor-infiltrating lymphocytes by the next generation sequencing and to elucidate the global picture of tumor immunity from the viewpoint of immuno-genetics. Additionally, I also aimed to create a basic database to develop new therapeutic strategies against cancers, such as novel anti-cancer antibodies, based on antigen receptor repertoire sequences that existed dominantly in cancer tissues. The following achievements were obtained.
(1) Next generation sequencing protocols for the antigen receptor repertoires of tumor-infiltrating lymphocytes in clinical cancer tissues were established. (2) A number of T cell receptors and immunoglobulins that were specifically and dominantly infiltrated in cancer tissues were identified. (3) Functional screening of multiple cancer specific immunoglobulin molecules reconstructed by artificial expression system could be carried out.
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