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Biochemical analysis of small RNA-induced epigenetic changes

Research Project

Project/Area Number 26710011
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypePartial Multi-year Fund
Research Field Genome biology
Research InstitutionKeio University

Principal Investigator

YAMANAKA SOICHIRO  慶應義塾大学, 医学部, 助教 (80711845)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥24,050,000 (Direct Cost: ¥18,500,000、Indirect Cost: ¥5,550,000)
Fiscal Year 2016: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2015: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2014: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Keywords小分子RNA / トランスポゾン / 生殖細胞 / ATAC-seq / ゲノム / CRISPR/Cas9 / リピート配列 / クロマチン / ヘテロクロマチン / piRNA / エピジェネティクス / 遺伝子発現制御
Outline of Final Research Achievements

More than 45% of human genome consists of transposons and its remnants. Transpositional events of them are deleterious to their hosts, especially in their gonads. Here, I am engaged in how small RNA would induce transcriptional silencing at transposon sequences, by using both fly and mouse systems. For fly system, I am trying to establish a method to proteomically identify the chromatin components at specific genomic region. For mouse system, I have profiled the chromatin dynamics in the small number of germ cells within the whole testis, and comprehensively described its temporal change in detail, particularly at transposon sequences.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (7 results)

All 2016 2015 2014 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (2 results) (of which Int'l Joint Research: 2 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Misprocessed tRNA response targets piRNA clusters2015

    • Author(s)
      Soichiro Yamanaka, Haruhiko Siomi
    • Journal Title

      EMBO J

      Volume: 34 Issue: 24 Pages: 2988-9

    • DOI

      10.15252/embj.201593322

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] piRNA clusters and open chromatin structure.2014

    • Author(s)
      Yamanaka, S., Siomi, MC. and Siomi, H.
    • Journal Title

      Mobile DNA

      Volume: 5 Issue: 1 Pages: 22-22

    • DOI

      10.1186/1759-8753-5-22

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Developing a novel method to identify chromatin components at transposons using CRISPR/Cas9 system2016

    • Author(s)
      Soichiro Yamanaka, Ten Li, Takeshi Kawamura, Tatsuhiko Kodama, Haruhiko Siomi
    • Organizer
      国際RNA学会
    • Place of Presentation
      京都、国際会議場
    • Year and Date
      2016-06-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Developing a novel method to identify chromatin components at transposons using CRISPR/Cas9 system2016

    • Author(s)
      Soichiro Yamanaka, Ten Li, Takeshi Kawamura, Tatsuhiko Kodama, Haruhiko Siomi
    • Organizer
      RNA2016 Meeting
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2016-06-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Book] 細胞工学2015

    • Author(s)
      山中総一郎、塩見春彦
    • Total Pages
      6
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2015 Annual Research Report
  • [Book] 実験医学2015

    • Author(s)
      山中総一郎
    • Total Pages
      2
    • Publisher
      羊土社
    • Related Report
      2015 Annual Research Report
  • [Remarks] 慶應義塾大学 医学部 分子生物学教室 ホームページ

    • URL

      http://siomilab.med.keio.ac.jp

    • Related Report
      2015 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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