| Budget Amount *help |
¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
Deep subsurface microbes likely contribute to global biogeochemical processes. However, very little is known about their biological entity, diversity and function. This study elucidated ecophysiology of uncultured archaea and bacteria inhabiting deep subsurface environments by using the environmental omics and advanced cultivation approaches as well as conventional methods. In particular, we found novel archaeal and bacterial lineages in the subsurface environments. Besides, we successfully reconstructed genomes of the uncultured microbes and revealed their potential metabolisms associated with carbon flux. We were further successful of cultivation of novel archaea and bacteria. One of the isolates is the first methanogic archaea able to produce methane from coal. This surprising discovery contributes to understanding natural gas origins and formation mechanisms, and infers that cultivation is still necessary to elucidate functions of uncultured microorganisms even in metagenomic era.
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