Epigenetic mechanism about immediate early transcribed genes in vascular endothelial cells
Project/Area Number |
26710013
|
Research Category |
Grant-in-Aid for Young Scientists (A)
|
Allocation Type | Partial Multi-year Fund |
Research Field |
Medical genome science
|
Research Institution | The University of Tokyo |
Principal Investigator |
KANKI YASUHARU 東京大学, アイソトープ総合センター, 助教 (00534869)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
|
Keywords | 血管新生 / エピゲノム / VEGF / ChIP-seq / ヒストン修飾 / 抗がん剤 / エピジェネティクス / プロテオミクス |
Outline of Final Research Achievements |
In solid tumor, cancer cells secrete vascular endothelial cell growth factor (VEGF) by themselves in order to get oxygen and nutrition. In this report, our aim is to elucidate a novel epigenetic mechanism about immediate early transcribed genes in vascular endothelial cells under VEGF stimulation. At first, we performed ChIP-sequence by using various histone modification antibodies including H3K4me3, H3K27me3, H3K27ac, and H2AK119Ub. We clarified that H3K4me3 modification was induced within 15min on EGR3 locus. In addition, the inhibition of H3K4me3 modification by PTIP knockdown caused the reduction of VEGF-induced genes transcription in vitro and in vivo. Taken together, these findings have suggested that inhibition of PTIP might be useful material for the development of anti-angiogenic drugs in the future.
|
Report
(4 results)
Research Products
(16 results)
-
-
[Journal Article] Dynamically and epigenetically coordinated GATA/ETS/SOX transcription is indispensable for endothelial cell differentiation2017
Author(s)
Kanki Y, Nakaki R, Shimamura T, Matsunaga T, Yamamizu K, Katayama S, Suehiro JI, Osawa T, Aburatani H, Kodama T, Wada Y, Yamashita JK, Minami T.
-
Journal Title
Nucleic Acids Research
Volume: 印刷中
Issue: 8
Pages: 4344-4358
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
[Journal Article] Effects of Chronic Low-Dose Radiation on Human Neural Progenitor Cells.2016
Author(s)
Katsura M, Cyou-Nakamine H, Zen Q, Zen Y, Nansai H, Amagasa S, Kanki Y, Inoue T, Kaneki K, Taguchi A, Kobayashi M, Kaji T, Kodama T, Miyagawa K, Wada Y, Akimitsu N, Sone H.
-
Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 20027-20027
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] Direct Evidence for Pitavastatin Induced Chromatin Structure Change in the KLF4 Gene in Endothelial Cells.2014
Author(s)
Maejima T, Inoue T, Kanki Y, Kohro T, Li G, Ohta Y, Kimura H, Kobayashi M, Taguchi A, Tsutsumi S, Iwanari H, Yamamoto S, Aruga H, Dong S, Stevens JF, Poh HM, Yamamoto K, Kawamura T, Mimura I,
-
Journal Title
PLoS One.
Volume: 9
Issue: 5
Pages: e96005-e96005
DOI
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Cross-enhancement of ANGPTL4 transcription by HIF1 alpha and PPAR beta/delta is the result of the conformational proximity of two response elements.2014
Author(s)
Inoue T, Kohro T, Tanaka T, Kanki Y, Li G, Poh HM, Mimura I, Kobayashi M, Taguchi A, Maejima T, Suehiro JI, Sugiyama A, Kaneki K, Aruga H, Dong S, Stevens JF, Yamamoto S, Tsutsumi S, Fujita T, Ruan X, Aburatani H, Nangaku M, Ruan Y, Kodama T, Wada Y.
-
Journal Title
Genome Biology
Volume: 15
Issue: 4
Pages: R63-R63
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
[Presentation] GENETIC AND EPIGENETIC LANDSCAPE OF ENDOTHELIAL CELLS DIFFERENTITATION REVEAL THE TRANSCRIPTIONAL FACTORS NETWORK2014
Author(s)
Kanki, Yasuharu, Matsunaga, Taichi, Ryo, Nakaki, Yamamizu, Kohei, Shimamura, Teppei, Miyano, Satoru, Aburatani, Hiroyuki, Kodama, Tatsuhiko, Wada, Youichiro, Yamashita, Jun, Minami, Takashi
Organizer
International Society For Stem Cell Research the 12th annual meeting
Place of Presentation
The Convention Center, Vancouver, Canada
Year and Date
2014-06-18 – 2014-06-21
Related Report
-
-