| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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| Outline of Final Research Achievements |
In solid tumor, cancer cells secrete vascular endothelial cell growth factor (VEGF) by themselves in order to get oxygen and nutrition. In this report, our aim is to elucidate a novel epigenetic mechanism about immediate early transcribed genes in vascular endothelial cells under VEGF stimulation.
At first, we performed ChIP-sequence by using various histone modification antibodies including H3K4me3, H3K27me3, H3K27ac, and H2AK119Ub. We clarified that H3K4me3 modification was induced within 15min on EGR3 locus. In addition, the inhibition of H3K4me3 modification by PTIP knockdown caused the reduction of VEGF-induced genes transcription in vitro and in vivo.
Taken together, these findings have suggested that inhibition of PTIP might be useful material for the development of anti-angiogenic drugs in the future.
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