Development of the self-replicable cell-free protein synthesis system
| Project/Area Number |
26710014
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (A)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Research Field |
System genome science
|
|---|
| Research Institution | Institute of Physical and Chemical Research |
|---|
Principal Investigator |
Shimizu Yoshihiro 国立研究開発法人理化学研究所, 生命システム研究センター, ユニットリーダー (90401231)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
|
|---|
| Keywords | 無細胞タンパク質合成 / リボソーム / tRNA / マイクロ流路 / 生体分子 / 蛋白質 |
|---|
| Outline of Final Research Achievements |
To achieve the reconstruction of life by a combination of biomolecules, it is considered essential to reconstitute the means for producing proteins themselves in a self-replicable state. In this research, to achieve self-replication of the translation system by itself, we regarded the PURE system, a reconstituted cell-free protein synthesis system, as a fundamental system for the reconstruction of life and developed each technological basis for ribosome reconstitution, tRNA reconstitution, and protein synthesis in a microchannel.
|
Report
(4 results)
Research Products
(21 results)
-
-
-
-
[Journal Article] Mammalian Reverse Genetics without Crossing Reveals Nr3a as a Short-Sleeper Gene.2016
Author(s)
Sunagawa GA, Sumiyama K, Ukai-Tadenuma M, Perrin D, Fujishima H, Ukai H, Nishimura O, Shi S, Ohno R, Narumi R, Shimizu Y, Tone D, Ode KL, Kuraku S, Ueda HR.
-
Journal Title
Cell Rep.
Volume: 14
Issue: 3
Pages: 662-677
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
[Journal Article] Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis.2014
Author(s)
Etsuo A. Susaki, Kazuki Tainaka, Dimitri Perrin, Fumiaki Kishino, Takehiro Tawara, Tomonobu M. Watanabe, Chihiro Yokoyama, Hirotaka Onoe, Megumi Eguchi, Shun Yamaguchi, Takaya Abe, Hiroshi Kiyonari, Yoshihiro Shimizu, Atsushi Miyawaki, Hideo Yokota, Hiroki R. Ueda
-
Journal Title
Cell
Volume: 157
Issue: 3
Pages: 726-739
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
