| Budget Amount *help |
¥23,400,000 (Direct Cost: ¥18,000,000、Indirect Cost: ¥5,400,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Outline of Final Research Achievements |
To achieve the reconstruction of life by a combination of biomolecules, it is considered essential to reconstitute the means for producing proteins themselves in a self-replicable state. In this research, to achieve self-replication of the translation system by itself, we regarded the PURE system, a reconstituted cell-free protein synthesis system, as a fundamental system for the reconstruction of life and developed each technological basis for ribosome reconstitution, tRNA reconstitution, and protein synthesis in a microchannel.
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